Intelligent nanotherapeutic strategies for the delivery of CRISPR system
CRISPR, as an emerging gene editing technology, has been widely used in multiple fields due to its convenient operation, less cost, high efficiency and precision. This robust and effective device has revolutionized the development of biomedical research at an unexpected speed in recent years. The de...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383522005226 |
| _version_ | 1827913060513218560 |
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| author | Chao Chen Wu Zhong Shiyu Du Yayao Li Yunfei Zeng Kunguo Liu Jingjing Yang Xiaoxiang Guan Xin Han |
| author_facet | Chao Chen Wu Zhong Shiyu Du Yayao Li Yunfei Zeng Kunguo Liu Jingjing Yang Xiaoxiang Guan Xin Han |
| author_sort | Chao Chen |
| collection | DOAJ |
| description | CRISPR, as an emerging gene editing technology, has been widely used in multiple fields due to its convenient operation, less cost, high efficiency and precision. This robust and effective device has revolutionized the development of biomedical research at an unexpected speed in recent years. The development of intelligent and precise CRISPR delivery strategies in a controllable and safe manner is the prerequisite for translational clinical medicine in gene therapy field. In this review, the therapeutic application of CRISPR delivery and the translational potential of gene editing was firstly discussed. Critical obstacles for the delivery of CRISPR system in vivo and shortcomings of CRISPR system itself were also analyzed. Given that intelligent nanoparticles have demonstrated great potential on the delivery of CRISPR system, here we mainly focused on stimuli-responsive nanocarriers. We also summarized various strategies for CIRSPR-Cas9 system delivered by intelligent nanocarriers which would respond to different endogenous and exogenous signal stimulus. Moreover, new genome editors mediated by nanotherapeutic vectors for gene therapy were also discussed. Finally, we discussed future prospects of genome editing for existing nanocarriers in clinical settings. |
| first_indexed | 2024-03-13T02:25:03Z |
| format | Article |
| id | doaj.art-5c0033f60b8a49b8bc2830be90a2c6e1 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 |
| language | English |
| last_indexed | 2024-03-13T02:25:03Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-5c0033f60b8a49b8bc2830be90a2c6e12023-06-30T04:21:56ZengElsevierActa Pharmaceutica Sinica B2211-38352023-06-0113625102543Intelligent nanotherapeutic strategies for the delivery of CRISPR systemChao Chen0Wu Zhong1Shiyu Du2Yayao Li3Yunfei Zeng4Kunguo Liu5Jingjing Yang6Xiaoxiang Guan7Xin Han8Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaKey Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha 410013, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaKey Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha 410013, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaDepartment of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; Corresponding authors.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; Corresponding authors.CRISPR, as an emerging gene editing technology, has been widely used in multiple fields due to its convenient operation, less cost, high efficiency and precision. This robust and effective device has revolutionized the development of biomedical research at an unexpected speed in recent years. The development of intelligent and precise CRISPR delivery strategies in a controllable and safe manner is the prerequisite for translational clinical medicine in gene therapy field. In this review, the therapeutic application of CRISPR delivery and the translational potential of gene editing was firstly discussed. Critical obstacles for the delivery of CRISPR system in vivo and shortcomings of CRISPR system itself were also analyzed. Given that intelligent nanoparticles have demonstrated great potential on the delivery of CRISPR system, here we mainly focused on stimuli-responsive nanocarriers. We also summarized various strategies for CIRSPR-Cas9 system delivered by intelligent nanocarriers which would respond to different endogenous and exogenous signal stimulus. Moreover, new genome editors mediated by nanotherapeutic vectors for gene therapy were also discussed. Finally, we discussed future prospects of genome editing for existing nanocarriers in clinical settings.http://www.sciencedirect.com/science/article/pii/S2211383522005226CRISPR systemIntelligent deliveryGene editingEndogenous responsiveExogenous controlNanotherapeutic platforms |
| spellingShingle | Chao Chen Wu Zhong Shiyu Du Yayao Li Yunfei Zeng Kunguo Liu Jingjing Yang Xiaoxiang Guan Xin Han Intelligent nanotherapeutic strategies for the delivery of CRISPR system Acta Pharmaceutica Sinica B CRISPR system Intelligent delivery Gene editing Endogenous responsive Exogenous control Nanotherapeutic platforms |
| title | Intelligent nanotherapeutic strategies for the delivery of CRISPR system |
| title_full | Intelligent nanotherapeutic strategies for the delivery of CRISPR system |
| title_fullStr | Intelligent nanotherapeutic strategies for the delivery of CRISPR system |
| title_full_unstemmed | Intelligent nanotherapeutic strategies for the delivery of CRISPR system |
| title_short | Intelligent nanotherapeutic strategies for the delivery of CRISPR system |
| title_sort | intelligent nanotherapeutic strategies for the delivery of crispr system |
| topic | CRISPR system Intelligent delivery Gene editing Endogenous responsive Exogenous control Nanotherapeutic platforms |
| url | http://www.sciencedirect.com/science/article/pii/S2211383522005226 |
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