Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria
Antibodies of the secretory IgA (SIgA) class comprise the first line of antigen-specific immune defense, preventing access of commensal and pathogenic microorganisms and their secreted products into the body proper. In addition to preventing infection, SIgA shapes the composition of the gut microbi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-04-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2076-0817/3/2/390 |
| _version_ | 1798042382315290624 |
|---|---|
| author | Eric W. Rogier Aubrey L. Frantz Maria E. C. Bruno Charlotte S. Kaetzel |
| author_facet | Eric W. Rogier Aubrey L. Frantz Maria E. C. Bruno Charlotte S. Kaetzel |
| author_sort | Eric W. Rogier |
| collection | DOAJ |
| description | Antibodies of the secretory IgA (SIgA) class comprise the first line of antigen-specific immune defense, preventing access of commensal and pathogenic microorganisms and their secreted products into the body proper. In addition to preventing infection, SIgA shapes the composition of the gut microbiome. SIgA is transported across intestinal epithelial cells into gut secretions by the polymeric immunoglobulin receptor (pIgR). The epithelial surface is protected by a thick network of mucus, which is composed of a dense, sterile inner layer and a loose outer layer that is colonized by commensal bacteria. Immunofluorescence microscopy of mouse and human colon tissues demonstrated that the SIgA co-localizes with gut bacteria in the outer mucus layer. Using mice genetically deficient for pIgR and/or mucin-2 (Muc2, the major glycoprotein of intestinal mucus), we found that Muc2 but not SIgA was necessary for excluding gut bacteria from the inner mucus layer in the colon. Our findings support a model whereby SIgA is anchored in the outer layer of colonic mucus through combined interactions with mucin proteins and gut bacteria, thus providing immune protection against pathogens while maintaining a mutually beneficial relationship with commensals. |
| first_indexed | 2024-04-11T22:34:43Z |
| format | Article |
| id | doaj.art-5c005aeac8d542238900b88861611cd2 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T22:34:43Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-5c005aeac8d542238900b88861611cd22022-12-22T03:59:15ZengMDPI AGPathogens2076-08172014-04-013239040310.3390/pathogens3020390pathogens3020390Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut BacteriaEric W. Rogier0Aubrey L. Frantz1Maria E. C. Bruno2Charlotte S. Kaetzel3Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536, USADepartment of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536, USADepartment of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536, USADepartment of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536, USAAntibodies of the secretory IgA (SIgA) class comprise the first line of antigen-specific immune defense, preventing access of commensal and pathogenic microorganisms and their secreted products into the body proper. In addition to preventing infection, SIgA shapes the composition of the gut microbiome. SIgA is transported across intestinal epithelial cells into gut secretions by the polymeric immunoglobulin receptor (pIgR). The epithelial surface is protected by a thick network of mucus, which is composed of a dense, sterile inner layer and a loose outer layer that is colonized by commensal bacteria. Immunofluorescence microscopy of mouse and human colon tissues demonstrated that the SIgA co-localizes with gut bacteria in the outer mucus layer. Using mice genetically deficient for pIgR and/or mucin-2 (Muc2, the major glycoprotein of intestinal mucus), we found that Muc2 but not SIgA was necessary for excluding gut bacteria from the inner mucus layer in the colon. Our findings support a model whereby SIgA is anchored in the outer layer of colonic mucus through combined interactions with mucin proteins and gut bacteria, thus providing immune protection against pathogens while maintaining a mutually beneficial relationship with commensals.http://www.mdpi.com/2076-0817/3/2/390intestinal epitheliumgut bacteriasecretory IgApolymeric immunoglobulin receptor (pIgR)intestinal mucusmucin-2 |
| spellingShingle | Eric W. Rogier Aubrey L. Frantz Maria E. C. Bruno Charlotte S. Kaetzel Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria Pathogens intestinal epithelium gut bacteria secretory IgA polymeric immunoglobulin receptor (pIgR) intestinal mucus mucin-2 |
| title | Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria |
| title_full | Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria |
| title_fullStr | Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria |
| title_full_unstemmed | Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria |
| title_short | Secretory IgA is Concentrated in the Outer Layer of Colonic Mucus along with Gut Bacteria |
| title_sort | secretory iga is concentrated in the outer layer of colonic mucus along with gut bacteria |
| topic | intestinal epithelium gut bacteria secretory IgA polymeric immunoglobulin receptor (pIgR) intestinal mucus mucin-2 |
| url | http://www.mdpi.com/2076-0817/3/2/390 |
| work_keys_str_mv | AT ericwrogier secretoryigaisconcentratedintheouterlayerofcolonicmucusalongwithgutbacteria AT aubreylfrantz secretoryigaisconcentratedintheouterlayerofcolonicmucusalongwithgutbacteria AT mariaecbruno secretoryigaisconcentratedintheouterlayerofcolonicmucusalongwithgutbacteria AT charlotteskaetzel secretoryigaisconcentratedintheouterlayerofcolonicmucusalongwithgutbacteria |