Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin
Objective(s): The purpose of this study was preparation and evaluation of PLGA nanospheres containing the influenza virus and different adjuvants, Quillaja saponin (QS) and CpG-ODN. Materials and Methods: Nanospheres were prepared using the double emulsion-solvent evaporation method. The morphol...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_3340_468f263ef8bdda3103451e9572c25e80.html |
| _version_ | 1828408354212413440 |
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| author | Maryam Mohajer Bahman Khameneh Mohsen Tafaghodi |
| author_facet | Maryam Mohajer Bahman Khameneh Mohsen Tafaghodi |
| author_sort | Maryam Mohajer |
| collection | DOAJ |
| description | Objective(s):
The purpose of this study was preparation and evaluation of PLGA nanospheres containing the influenza virus and different adjuvants, Quillaja saponin (QS) and CpG-ODN.
Materials and Methods:
Nanospheres were prepared using the double emulsion-solvent evaporation method. The morphological and physicochemical properties were studied by scanning electron microscopy (SEM), determination of zeta potential, encapsulation efficiency and release profile.
Results:
The particle size of formulations was less than 1000 nm, except for formulations containing antigen. The results were confirmed with SEM images. Encapsulation efficiency of antigen, QS and CpG ODN were 80%, 62% and 31%, respectively. The zeta potential of nanospheres was about -30 mV. The burst release was observed for all encapsulates and reached to about 48%, 44% and 35% within 90 min for antigen, CpG-ODN and Qs content, respectively.
Conclusion:
The formulations showed proper physicochemical properties. These nanospheres have good potential to be used as delivery systems/adjuvants for immunization against influenza. |
| first_indexed | 2024-12-10T11:37:25Z |
| format | Article |
| id | doaj.art-5c00b4ff56fe4fab87355ae3fa46d398 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-10T11:37:25Z |
| publishDate | 2014-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-5c00b4ff56fe4fab87355ae3fa46d3982022-12-22T01:50:22ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-09-011797227263340Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponinMaryam Mohajer0Bahman Khameneh1Mohsen Tafaghodi2Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Food and Drug Control, Students Research Committee, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): The purpose of this study was preparation and evaluation of PLGA nanospheres containing the influenza virus and different adjuvants, Quillaja saponin (QS) and CpG-ODN. Materials and Methods: Nanospheres were prepared using the double emulsion-solvent evaporation method. The morphological and physicochemical properties were studied by scanning electron microscopy (SEM), determination of zeta potential, encapsulation efficiency and release profile. Results: The particle size of formulations was less than 1000 nm, except for formulations containing antigen. The results were confirmed with SEM images. Encapsulation efficiency of antigen, QS and CpG ODN were 80%, 62% and 31%, respectively. The zeta potential of nanospheres was about -30 mV. The burst release was observed for all encapsulates and reached to about 48%, 44% and 35% within 90 min for antigen, CpG-ODN and Qs content, respectively. Conclusion: The formulations showed proper physicochemical properties. These nanospheres have good potential to be used as delivery systems/adjuvants for immunization against influenza.http://ijbms.mums.ac.ir/pdf_3340_468f263ef8bdda3103451e9572c25e80.htmlCpG-ODNInfluenza antigenPLGA nanospheresQuillaja saponin |
| spellingShingle | Maryam Mohajer Bahman Khameneh Mohsen Tafaghodi Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin Iranian Journal of Basic Medical Sciences CpG-ODN Influenza antigen PLGA nanospheres Quillaja saponin |
| title | Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin |
| title_full | Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin |
| title_fullStr | Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin |
| title_full_unstemmed | Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin |
| title_short | Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin |
| title_sort | preparation and characterization of plga nanospheres loaded with inactivated influenza virus cpg odn and quillaja saponin |
| topic | CpG-ODN Influenza antigen PLGA nanospheres Quillaja saponin |
| url | http://ijbms.mums.ac.ir/pdf_3340_468f263ef8bdda3103451e9572c25e80.html |
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