Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949)
The objective of this study was to evaluate and compare the associations between TAS2R16 serum levels and common gene rs860170, rs978739, and rs1357949 polymorphisms in patients affected by generalized periodontitis. The study enrolled 590 patients: 280 patients with periodontitis and 310 healthy co...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2227-9059/12/2/319 |
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| author | Albertas Kriauciunas Greta Gedvilaite Akvile Bruzaite Gediminas Zekonis Dainius Razukevicius Rasa Liutkeviciene |
| author_facet | Albertas Kriauciunas Greta Gedvilaite Akvile Bruzaite Gediminas Zekonis Dainius Razukevicius Rasa Liutkeviciene |
| author_sort | Albertas Kriauciunas |
| collection | DOAJ |
| description | The objective of this study was to evaluate and compare the associations between TAS2R16 serum levels and common gene rs860170, rs978739, and rs1357949 polymorphisms in patients affected by generalized periodontitis. The study enrolled 590 patients: 280 patients with periodontitis and 310 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNA salting-out method was used for DNA extraction from peripheral venous blood. Genotyping of <i>TAS2R16</i> (rs860170, rs978739, and rs1357949) was performed using real-time polymerase chain reaction (RT-PCR), and serum level analysis was performed for both periodontitis-affected patients and reference group subjects. The analysis of <i>TAS2R16</i> rs860170 (TT, CT, and CC) showed a statistically significant difference between generalized periodontitis and the reference group (41.8%, 58.2%, and 0% vs. 38.7%, 56.1%, and 5.2%, <i>p</i> < 0.001). <i>TAS2R16</i> rs860170 (TT, CT, and CC) showed a statistically significant difference between males in generalized periodontitis and reference groups (38.4%, 61.6%, and 0% vs. 32.9%, 56.6%, and 10.5%, <i>p</i> = 0.002). Female-specific analysis showed that the <i>TAS2R16</i> rs978739 C allele was more frequent in generalized periodontitis compared to the reference group (37.5% vs. 28.7%, <i>p</i> = 0.016). Subjects aged 70 years and older demonstrated a statistically significant difference in <i>TAS2R16</i> rs860170 (TT, CT, and CC) between generalized periodontitis and the reference group (42.8%, 57.2%, and 0% vs. 38.6%, 53.8%, and 7.6%, <i>p</i> = 0.003). TAS2R16 serum levels were elevated in generalized periodontitis compared to the reference group (0.112 (0.06) ng/mL vs. 0.075 (0.03) ng/mL, <i>p</i> = 0.002). Females carrying the <i>TAS2R16</i> rs978739 C allele were more prone to generalized periodontitis development. Associations were found between <i>TAS2R16</i> rs860170 polymorphisms, elevated TAS2R16 serum levels, and generalized periodontitis development. |
| first_indexed | 2024-03-07T22:41:38Z |
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| language | English |
| last_indexed | 2024-03-07T22:41:38Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-5c030e80824841059199915548e01e1a2024-02-23T15:08:28ZengMDPI AGBiomedicines2227-90592024-01-0112231910.3390/biomedicines12020319Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949)Albertas Kriauciunas0Greta Gedvilaite1Akvile Bruzaite2Gediminas Zekonis3Dainius Razukevicius4Rasa Liutkeviciene5Department of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaDepartment of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, LithuaniaDepartment of Oral and Maxillofacial Surgery, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50161 Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaThe objective of this study was to evaluate and compare the associations between TAS2R16 serum levels and common gene rs860170, rs978739, and rs1357949 polymorphisms in patients affected by generalized periodontitis. The study enrolled 590 patients: 280 patients with periodontitis and 310 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNA salting-out method was used for DNA extraction from peripheral venous blood. Genotyping of <i>TAS2R16</i> (rs860170, rs978739, and rs1357949) was performed using real-time polymerase chain reaction (RT-PCR), and serum level analysis was performed for both periodontitis-affected patients and reference group subjects. The analysis of <i>TAS2R16</i> rs860170 (TT, CT, and CC) showed a statistically significant difference between generalized periodontitis and the reference group (41.8%, 58.2%, and 0% vs. 38.7%, 56.1%, and 5.2%, <i>p</i> < 0.001). <i>TAS2R16</i> rs860170 (TT, CT, and CC) showed a statistically significant difference between males in generalized periodontitis and reference groups (38.4%, 61.6%, and 0% vs. 32.9%, 56.6%, and 10.5%, <i>p</i> = 0.002). Female-specific analysis showed that the <i>TAS2R16</i> rs978739 C allele was more frequent in generalized periodontitis compared to the reference group (37.5% vs. 28.7%, <i>p</i> = 0.016). Subjects aged 70 years and older demonstrated a statistically significant difference in <i>TAS2R16</i> rs860170 (TT, CT, and CC) between generalized periodontitis and the reference group (42.8%, 57.2%, and 0% vs. 38.6%, 53.8%, and 7.6%, <i>p</i> = 0.003). TAS2R16 serum levels were elevated in generalized periodontitis compared to the reference group (0.112 (0.06) ng/mL vs. 0.075 (0.03) ng/mL, <i>p</i> = 0.002). Females carrying the <i>TAS2R16</i> rs978739 C allele were more prone to generalized periodontitis development. Associations were found between <i>TAS2R16</i> rs860170 polymorphisms, elevated TAS2R16 serum levels, and generalized periodontitis development.https://www.mdpi.com/2227-9059/12/2/319TAS2R16polymorphismsserum levelsperiodontitis |
| spellingShingle | Albertas Kriauciunas Greta Gedvilaite Akvile Bruzaite Gediminas Zekonis Dainius Razukevicius Rasa Liutkeviciene Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) Biomedicines TAS2R16 polymorphisms serum levels periodontitis |
| title | Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) |
| title_full | Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) |
| title_fullStr | Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) |
| title_full_unstemmed | Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) |
| title_short | Generalised Periodontitis: Examining TAS2R16 Serum Levels and Common Gene Polymorphisms (rs860170, rs978739, rs1357949) |
| title_sort | generalised periodontitis examining tas2r16 serum levels and common gene polymorphisms rs860170 rs978739 rs1357949 |
| topic | TAS2R16 polymorphisms serum levels periodontitis |
| url | https://www.mdpi.com/2227-9059/12/2/319 |
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