Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis
Commercial producers of white button mushrooms utilise a casing material to cover the spawn run compost, which stimulates the mushrooms' reproductive stage. Certain bacteria in this casing are responsible for this stimulation, which is known as pinning. Bacterial species richness and diversity...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of Science of South Africa
2010-10-01
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| Series: | South African Journal of Science |
| Online Access: | https://www.sajs.co.za/article/view/10116 |
| _version_ | 1818295412901019648 |
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| author | Nazareth Siyoum Karen Surridge Lise Korsten |
| author_facet | Nazareth Siyoum Karen Surridge Lise Korsten |
| author_sort | Nazareth Siyoum |
| collection | DOAJ |
| description | Commercial producers of white button mushrooms utilise a casing material to cover the spawn run compost, which stimulates the mushrooms' reproductive stage. Certain bacteria in this casing are responsible for this stimulation, which is known as pinning. Bacterial species richness and diversity within peat and peat-based casing mixtures made from industrial waste materials (i.e. those containing coir, wattle bark, bagasse and filter cake) were examined using denaturing gradient gel electrophoresis (DGGE) at three phases of mushroom growth: (1) casing, (2) pinning and (3) harvesting. Results from the DGGE established that higher bacterial species richness occurred at pinning and harvesting than at casing. Increases in bacterial population density at pinning were greater in the peat-based mixtures, which contained industrial waste materials, than in peat alone. Peat mixtures containing these alternative materials are therefore favourable substrates for bacterial growth. The DGGE profiles for pasteurised casing materials reflected their ability to rapidly re-establish the original bacterial community. The bacteria found to be dominant in casing materials during pinning were closely related to Pseudomonas, Flavobacterium, alpha-Proteobacterium, beta-Proteobacterium, gamma-Proteobacterium, delta-Proteobacterium and uncultured species. |
| first_indexed | 2024-12-13T03:47:14Z |
| format | Article |
| id | doaj.art-5c030f680dad42929e03016fd149724d |
| institution | Directory Open Access Journal |
| issn | 1996-7489 |
| language | English |
| last_indexed | 2024-12-13T03:47:14Z |
| publishDate | 2010-10-01 |
| publisher | Academy of Science of South Africa |
| record_format | Article |
| series | South African Journal of Science |
| spelling | doaj.art-5c030f680dad42929e03016fd149724d2022-12-22T00:00:47ZengAcademy of Science of South AfricaSouth African Journal of Science1996-74892010-10-011069/10Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresisNazareth Siyoum0Karen Surridge1Lise Korsten2University of PretoriaUniversity of PretoriaUniversity of PretoriaCommercial producers of white button mushrooms utilise a casing material to cover the spawn run compost, which stimulates the mushrooms' reproductive stage. Certain bacteria in this casing are responsible for this stimulation, which is known as pinning. Bacterial species richness and diversity within peat and peat-based casing mixtures made from industrial waste materials (i.e. those containing coir, wattle bark, bagasse and filter cake) were examined using denaturing gradient gel electrophoresis (DGGE) at three phases of mushroom growth: (1) casing, (2) pinning and (3) harvesting. Results from the DGGE established that higher bacterial species richness occurred at pinning and harvesting than at casing. Increases in bacterial population density at pinning were greater in the peat-based mixtures, which contained industrial waste materials, than in peat alone. Peat mixtures containing these alternative materials are therefore favourable substrates for bacterial growth. The DGGE profiles for pasteurised casing materials reflected their ability to rapidly re-establish the original bacterial community. The bacteria found to be dominant in casing materials during pinning were closely related to Pseudomonas, Flavobacterium, alpha-Proteobacterium, beta-Proteobacterium, gamma-Proteobacterium, delta-Proteobacterium and uncultured species.https://www.sajs.co.za/article/view/10116 |
| spellingShingle | Nazareth Siyoum Karen Surridge Lise Korsten Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis South African Journal of Science |
| title | Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis |
| title_full | Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis |
| title_fullStr | Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis |
| title_full_unstemmed | Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis |
| title_short | Bacterial profiling of casing materials for white button mushrooms (Agaricus bisporus) using denaturing gradient gel electrophoresis |
| title_sort | bacterial profiling of casing materials for white button mushrooms agaricus bisporus using denaturing gradient gel electrophoresis |
| url | https://www.sajs.co.za/article/view/10116 |
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