Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice
Salinity stress is one of the most problematic constraints to significantly reduce rice productivity. The <i>Saltol</i> QTL (quantitative trait locus) has been known as one among many principal genes/QTLs responsible for salinity tolerance in rice. However, the introgression of the <i...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/7/1032 |
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| author | Can Thu Huong Truong Thi Tu Anh Tran Dang Dat Tran Dang Khanh Tran Dang Xuan |
| author_facet | Can Thu Huong Truong Thi Tu Anh Tran Dang Dat Tran Dang Khanh Tran Dang Xuan |
| author_sort | Can Thu Huong |
| collection | DOAJ |
| description | Salinity stress is one of the most problematic constraints to significantly reduce rice productivity. The <i>Saltol</i> QTL (quantitative trait locus) has been known as one among many principal genes/QTLs responsible for salinity tolerance in rice. However, the introgression of the <i>Saltol</i> QTL from the donor (male) into the recipient (female) cultivars induces great recessions from the progeny generation, which results in heavy fieldwork and greater cost and time required for breeding. In this study, the F<sub>1</sub> generation of the cross TBR1 (female cultivar, salinity tolerant) × KD18 (male cultivar, salinity susceptible) was preliminarily treated with N-methyl-N-nitrosourea (MNU) to induce the mutants M<sub>1</sub>. Results on physiological traits show that all the M<sub>2</sub> (self-pollinated from M1) and M<sub>3</sub> (self-pollinated from M2) individuals obtain salinity tolerant levels as the recurrent TBR1. Twelve SSR (simple sequence repeat) markers involved in the <i>Saltol</i> QTL (RM493, RM562, RM10694, RM10720, RM10793, RM10852, RM13197, RM201, RM149, RM508, RM587, and RM589) and other markers related to yield-contributing traits and disease resistance, as well as water and nitrogen use, have efficacy that is polymorphic. The phenotype and genotype analyses indicate that the salinity tolerant <i>Saltol</i> QTL, growth parameter, grain yield and quality, pest resistance, water and nitrogen use efficacy, and beneficial phytochemicals including antioxidants, momilactone A (MA) and momilactone B (MB) are uniparentally inherited from the recurrent (female) TBR1 cultivar and stabilized in the M<sub>2</sub> and M<sub>3</sub> generations. Further MNU applications should be examined to induce the uniparental inheritance of other salinity tolerant genes such as <i>OsCPK</i>17, <i>OsRMC</i>, <i>OsNHX</i>1, <i>OsHKT</i>1;5 to target rice cultivars. However, the mechanism of inducing this novel uniparental inheritance for salinity tolerance by MNU application needs elaboration. |
| first_indexed | 2024-03-10T18:24:40Z |
| format | Article |
| id | doaj.art-5c0365171a494b5aadb8c3d5311559db |
| institution | Directory Open Access Journal |
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| publishDate | 2020-07-01 |
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| spelling | doaj.art-5c0365171a494b5aadb8c3d5311559db2023-11-20T07:08:08ZengMDPI AGAgronomy2073-43952020-07-01107103210.3390/agronomy10071032Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in RiceCan Thu Huong0Truong Thi Tu Anh1Tran Dang Dat2Tran Dang Khanh3Tran Dang Xuan4Graduated School for Advanced Science and Engineering, Hiroshima University, 1-5-1 Kagamiyama, Higashihiroshima 739-8529, JapanDivision of Biotechnology, Institute of Agricultural Science for Southern Vietnam, 121 Nguyen, Binh Khiem Street, District 1, Ho Chi Minh City 71058, VietnamKhai Xuan International Co. Ltd., Ha Dong District, Duong Noi Ward, LK20A-20B, Khai Xuan Building, Hanoi 152611, VietnamDepartment of Genetic Engineering, Agricultural Genetics Institute, Hanoi 122300, VietnamGraduated School for Advanced Science and Engineering, Hiroshima University, 1-5-1 Kagamiyama, Higashihiroshima 739-8529, JapanSalinity stress is one of the most problematic constraints to significantly reduce rice productivity. The <i>Saltol</i> QTL (quantitative trait locus) has been known as one among many principal genes/QTLs responsible for salinity tolerance in rice. However, the introgression of the <i>Saltol</i> QTL from the donor (male) into the recipient (female) cultivars induces great recessions from the progeny generation, which results in heavy fieldwork and greater cost and time required for breeding. In this study, the F<sub>1</sub> generation of the cross TBR1 (female cultivar, salinity tolerant) × KD18 (male cultivar, salinity susceptible) was preliminarily treated with N-methyl-N-nitrosourea (MNU) to induce the mutants M<sub>1</sub>. Results on physiological traits show that all the M<sub>2</sub> (self-pollinated from M1) and M<sub>3</sub> (self-pollinated from M2) individuals obtain salinity tolerant levels as the recurrent TBR1. Twelve SSR (simple sequence repeat) markers involved in the <i>Saltol</i> QTL (RM493, RM562, RM10694, RM10720, RM10793, RM10852, RM13197, RM201, RM149, RM508, RM587, and RM589) and other markers related to yield-contributing traits and disease resistance, as well as water and nitrogen use, have efficacy that is polymorphic. The phenotype and genotype analyses indicate that the salinity tolerant <i>Saltol</i> QTL, growth parameter, grain yield and quality, pest resistance, water and nitrogen use efficacy, and beneficial phytochemicals including antioxidants, momilactone A (MA) and momilactone B (MB) are uniparentally inherited from the recurrent (female) TBR1 cultivar and stabilized in the M<sub>2</sub> and M<sub>3</sub> generations. Further MNU applications should be examined to induce the uniparental inheritance of other salinity tolerant genes such as <i>OsCPK</i>17, <i>OsRMC</i>, <i>OsNHX</i>1, <i>OsHKT</i>1;5 to target rice cultivars. However, the mechanism of inducing this novel uniparental inheritance for salinity tolerance by MNU application needs elaboration.https://www.mdpi.com/2073-4395/10/7/1032ricesalinity toleranceMNU treatmentuniparental inheritanceQTLgene |
| spellingShingle | Can Thu Huong Truong Thi Tu Anh Tran Dang Dat Tran Dang Khanh Tran Dang Xuan Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice Agronomy rice salinity tolerance MNU treatment uniparental inheritance QTL gene |
| title | Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice |
| title_full | Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice |
| title_fullStr | Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice |
| title_full_unstemmed | Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice |
| title_short | Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice |
| title_sort | uniparental inheritance of salinity tolerance and beneficial phytochemicals in rice |
| topic | rice salinity tolerance MNU treatment uniparental inheritance QTL gene |
| url | https://www.mdpi.com/2073-4395/10/7/1032 |
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