Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices
The combination of pharmacologic and endoscopic therapies is the gold standard for treating intestinal failures. The possibility of chemical solubility in water is mandatory for intelligent capsules. Functionalised silk fibroin with peptides and covalently linking different molecular entities to its...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/14/4605 |
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| author | Luca Valentini Lorenzo Pacini Fosca Errante Cecilia Morchio Beatrice Sanna Paolo Rovero Antonino Morabito |
| author_facet | Luca Valentini Lorenzo Pacini Fosca Errante Cecilia Morchio Beatrice Sanna Paolo Rovero Antonino Morabito |
| author_sort | Luca Valentini |
| collection | DOAJ |
| description | The combination of pharmacologic and endoscopic therapies is the gold standard for treating intestinal failures. The possibility of chemical solubility in water is mandatory for intelligent capsules. Functionalised silk fibroin with peptides and covalently linking different molecular entities to its structure make this protein a platform for preparing gels dissolving in the small and large intestine for drug delivery. In the present study, we linked a peptide containing the cell-adhesive motif Arginine–Glycine–Aspartic acid (RGD) to degummed silk fibres (DSF). Regenerated silk fibroin (RS) films obtained by dissolving functionalised DSF in formic acid were used to prepare composite gelatin. We show that such composite gelatin remains stable and elastic in the simulated gastric fluid (SGF) but can dissolve in the small and large intestines’ neutral-pH simulated intestine fluid (SIF). These findings open up the possibility of designing microfabricated and physically programmable scaffolds that locally promote tissue regeneration, thanks to bio-enabled materials based on functionalised regenerated silk. |
| first_indexed | 2024-03-09T13:17:25Z |
| format | Article |
| id | doaj.art-5c06f37058cb4597b3527762221fb28d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:17:25Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5c06f37058cb4597b3527762221fb28d2023-11-30T21:34:33ZengMDPI AGMolecules1420-30492022-07-012714460510.3390/molecules27144605Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible DevicesLuca Valentini0Lorenzo Pacini1Fosca Errante2Cecilia Morchio3Beatrice Sanna4Paolo Rovero5Antonino Morabito6Civil and Environmental Engineering Department, University of Perugia, Strada di Pentima 4, 05100 Terni, ItalyInterdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Chemistry “Ugo Schiff”, University of Florence, 59100 Sesto Fiorentino, ItalyInterdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of NeuroFarBa, University of Florence, 50019 Sesto Fiorentino, ItalyDipartimento Neuroscienze, Psicologia, Area del Farmaco e della Salute del Bambino NEUROFARBA, Università degli Studi di Firenze, Viale Pieraccini 6, 50121 Firenze, ItalyDipartimento Neuroscienze, Psicologia, Area del Farmaco e della Salute del Bambino NEUROFARBA, Università degli Studi di Firenze, Viale Pieraccini 6, 50121 Firenze, ItalyInterdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of NeuroFarBa, University of Florence, 50019 Sesto Fiorentino, ItalyDipartimento Neuroscienze, Psicologia, Area del Farmaco e della Salute del Bambino NEUROFARBA, Università degli Studi di Firenze, Viale Pieraccini 6, 50121 Firenze, ItalyThe combination of pharmacologic and endoscopic therapies is the gold standard for treating intestinal failures. The possibility of chemical solubility in water is mandatory for intelligent capsules. Functionalised silk fibroin with peptides and covalently linking different molecular entities to its structure make this protein a platform for preparing gels dissolving in the small and large intestine for drug delivery. In the present study, we linked a peptide containing the cell-adhesive motif Arginine–Glycine–Aspartic acid (RGD) to degummed silk fibres (DSF). Regenerated silk fibroin (RS) films obtained by dissolving functionalised DSF in formic acid were used to prepare composite gelatin. We show that such composite gelatin remains stable and elastic in the simulated gastric fluid (SGF) but can dissolve in the small and large intestines’ neutral-pH simulated intestine fluid (SIF). These findings open up the possibility of designing microfabricated and physically programmable scaffolds that locally promote tissue regeneration, thanks to bio-enabled materials based on functionalised regenerated silk.https://www.mdpi.com/1420-3049/27/14/4605peptidesilk fibroinprogrammable biomaterialsmechanical properties |
| spellingShingle | Luca Valentini Lorenzo Pacini Fosca Errante Cecilia Morchio Beatrice Sanna Paolo Rovero Antonino Morabito Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices Molecules peptide silk fibroin programmable biomaterials mechanical properties |
| title | Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices |
| title_full | Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices |
| title_fullStr | Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices |
| title_full_unstemmed | Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices |
| title_short | Peptide-Functionalized Silk Fibers as a Platform to Stabilize Gelatin for Use in Ingestible Devices |
| title_sort | peptide functionalized silk fibers as a platform to stabilize gelatin for use in ingestible devices |
| topic | peptide silk fibroin programmable biomaterials mechanical properties |
| url | https://www.mdpi.com/1420-3049/27/14/4605 |
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