Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil
The practice of feeding dogs raw meat-based diets (RMBDs) is growing in several countries, and the risks associated with the ingestion of pathogenic and antimicrobial-resistant <i>Escherichia coli</i> in dogs fed these diets are largely unknown. We characterized <i>E. coli</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/534 |
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| author | Carolina Pantuzza Ramos Carolina Yumi Iceri Kamei Flávia Mello Viegas Jonata de Melo Barbieri João Luís Reis Cunha Yaovi Mahuton Gildas Hounmanou Fernanda Morcatti Coura Jordana Almeida Santana Francisco Carlos Faria Lobato Anders Miki Bojesen Rodrigo Otávio Silveira Silva |
| author_facet | Carolina Pantuzza Ramos Carolina Yumi Iceri Kamei Flávia Mello Viegas Jonata de Melo Barbieri João Luís Reis Cunha Yaovi Mahuton Gildas Hounmanou Fernanda Morcatti Coura Jordana Almeida Santana Francisco Carlos Faria Lobato Anders Miki Bojesen Rodrigo Otávio Silveira Silva |
| author_sort | Carolina Pantuzza Ramos |
| collection | DOAJ |
| description | The practice of feeding dogs raw meat-based diets (RMBDs) is growing in several countries, and the risks associated with the ingestion of pathogenic and antimicrobial-resistant <i>Escherichia coli</i> in dogs fed these diets are largely unknown. We characterized <i>E. coli</i> strains isolated from dogs fed either an RMBD or a conventional dry feed, according to the phylogroup, virulence genes, and antimicrobial susceptibility profiles of the bacteria. Two hundred and sixteen <i>E. coli</i> strains were isolated. Dogs fed RMBDs shed <i>E. coli</i> strains from the phylogroup E more frequently and were positive for the <i>E. coli</i> heat-stable enterotoxin 1-encoding gene. Isolates from RMBD-fed dogs were also frequently positive for multidrug-resistant <i>E. coli</i> isolates including extended-spectrum beta-lactamase (ESBL) producers. Whole-genome sequencing of seven ESBL-producing <i>E. coli</i> strains revealed that they predominantly harbored <i>blaCTX-M-55</i>, and two strains were also positive for the colistin-resistant gene <i>mcr-1</i>. These results suggest that feeding an RMBD can affect the dog’s microbiota, change the frequency of certain phylogroups, and increase the shedding of diarrheagenic <i>E. coli</i>. Also, feeding an RMBD seemed to be linked with the fecal shedding of multidrug-resistant <i>E. coli</i>, including the spread of strains harboring mobilizable colistin resistance and ESBL genes. This finding is of concern for both animal and human health. |
| first_indexed | 2024-03-09T11:14:53Z |
| format | Article |
| id | doaj.art-5c08486c04e44114bdf3f85dcec45d86 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:14:53Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5c08486c04e44114bdf3f85dcec45d862023-12-01T00:33:32ZengMDPI AGAntibiotics2079-63822022-04-0111453410.3390/antibiotics11040534Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in BrazilCarolina Pantuzza Ramos0Carolina Yumi Iceri Kamei1Flávia Mello Viegas2Jonata de Melo Barbieri3João Luís Reis Cunha4Yaovi Mahuton Gildas Hounmanou5Fernanda Morcatti Coura6Jordana Almeida Santana7Francisco Carlos Faria Lobato8Anders Miki Bojesen9Rodrigo Otávio Silveira Silva10Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Copenhagen, DenmarkDepartamento de Ciências Agrárias, Instituto Federal de Minas Gerais (IFMG), Bambuí 38900-000, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Copenhagen, DenmarkDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30123-970, BrazilThe practice of feeding dogs raw meat-based diets (RMBDs) is growing in several countries, and the risks associated with the ingestion of pathogenic and antimicrobial-resistant <i>Escherichia coli</i> in dogs fed these diets are largely unknown. We characterized <i>E. coli</i> strains isolated from dogs fed either an RMBD or a conventional dry feed, according to the phylogroup, virulence genes, and antimicrobial susceptibility profiles of the bacteria. Two hundred and sixteen <i>E. coli</i> strains were isolated. Dogs fed RMBDs shed <i>E. coli</i> strains from the phylogroup E more frequently and were positive for the <i>E. coli</i> heat-stable enterotoxin 1-encoding gene. Isolates from RMBD-fed dogs were also frequently positive for multidrug-resistant <i>E. coli</i> isolates including extended-spectrum beta-lactamase (ESBL) producers. Whole-genome sequencing of seven ESBL-producing <i>E. coli</i> strains revealed that they predominantly harbored <i>blaCTX-M-55</i>, and two strains were also positive for the colistin-resistant gene <i>mcr-1</i>. These results suggest that feeding an RMBD can affect the dog’s microbiota, change the frequency of certain phylogroups, and increase the shedding of diarrheagenic <i>E. coli</i>. Also, feeding an RMBD seemed to be linked with the fecal shedding of multidrug-resistant <i>E. coli</i>, including the spread of strains harboring mobilizable colistin resistance and ESBL genes. This finding is of concern for both animal and human health.https://www.mdpi.com/2079-6382/11/4/534RMBDmultidrug-resistantEnterobacteriaceae |
| spellingShingle | Carolina Pantuzza Ramos Carolina Yumi Iceri Kamei Flávia Mello Viegas Jonata de Melo Barbieri João Luís Reis Cunha Yaovi Mahuton Gildas Hounmanou Fernanda Morcatti Coura Jordana Almeida Santana Francisco Carlos Faria Lobato Anders Miki Bojesen Rodrigo Otávio Silveira Silva Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil Antibiotics RMBD multidrug-resistant Enterobacteriaceae |
| title | Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil |
| title_full | Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil |
| title_fullStr | Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil |
| title_full_unstemmed | Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil |
| title_short | Fecal Shedding of Multidrug Resistant <i>Escherichia coli</i> Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil |
| title_sort | fecal shedding of multidrug resistant i escherichia coli i isolates in dogs fed with raw meat based diets in brazil |
| topic | RMBD multidrug-resistant Enterobacteriaceae |
| url | https://www.mdpi.com/2079-6382/11/4/534 |
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