A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma
Abstract Background Circular RNAs (circRNAs) are a class of noncoding RNAs that are involved in the progression of many human cancers. The precise gene locus and the roles of circular RNA from Fibronectin type III domain containing 3B (FNDC3B) in OS and its mechanisms of action have not been fully e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-023-03175-3 |
| _version_ | 1797376760063459328 |
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| author | Congya Li Linchao Ding Xuyao Wang Peng Shu Xuchao Shi Zhijian Zheng Jian Liu Junlan Zhu |
| author_facet | Congya Li Linchao Ding Xuyao Wang Peng Shu Xuchao Shi Zhijian Zheng Jian Liu Junlan Zhu |
| author_sort | Congya Li |
| collection | DOAJ |
| description | Abstract Background Circular RNAs (circRNAs) are a class of noncoding RNAs that are involved in the progression of many human cancers. The precise gene locus and the roles of circular RNA from Fibronectin type III domain containing 3B (FNDC3B) in OS and its mechanisms of action have not been fully explored. Materials and methods qRT-qPCR assay was used to determine gene expressions. CCK8 Assay, EdU assay, wound-healing assay, transwell invasion assay and in vivo xenograft assay were used to perform functional investigations. RNA-FISH, immunofluorescence, RIP assay, RNA stability analysis were applied in mechanistic studies. Results We found that circFNDC3B downregulated and FNDC3B mRNA upregulated in OS, and might be potential biomarkers for indicating disease progression and prognosis of OS patients. CircFNDC3B acted as a tumor suppressor gene to restrain OS progression and FNDC3B functioned as an oncogene to promote OS progression in vitro and in vivo. RNA binding protein RNA binding motif protein 47 (RBM47) could bind to the flanking introns of circFNDC3B to facilitate the generation of circFNDC3B, resulting in the reduction of FNDC3B mRNA and the circFNDC3B-FNDC3B mRNA imbalance. CircFNDC3B also inhibited FNDC3B mRNA expression by reducing its stability via competitively binding to Insulin-like growth-factor-2 mRNA binding protein (IGF2BP1). Conclusion This study demonstrated that RBM47 and IGF2BP1 mediated circular FNDC3B/FNDC3B mRNA imbalance was involved in the malignant processes of OS. |
| first_indexed | 2024-03-08T19:43:22Z |
| format | Article |
| id | doaj.art-5c09a989ac734f0b8749812bbb241eee |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-03-08T19:43:22Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
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| series | Cancer Cell International |
| spelling | doaj.art-5c09a989ac734f0b8749812bbb241eee2023-12-24T12:29:45ZengBMCCancer Cell International1475-28672023-12-0123111310.1186/s12935-023-03175-3A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcomaCongya Li0Linchao Ding1Xuyao Wang2Peng Shu3Xuchao Shi4Zhijian Zheng5Jian Liu6Junlan Zhu7Precision Medicine Laboratory, Beilun People’s Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang UniversityDepartment of Scientific Research, Affiliated Jinhua Hospital, Zhejiang University School of MedicinePrecision Medicine Laboratory, Beilun People’s Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang UniversityPrecision Medicine Laboratory, Beilun People’s Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang UniversityDepartment of Orthopaedics Surgery, Beilun People’s HospitalDepartment of Medical Oncology, Affiliated Jinhua Hospital, Zhejiang University School of MedicinePrecision Medicine Laboratory, Beilun People’s Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang UniversityPrecision Medicine Laboratory, Beilun People’s Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang UniversityAbstract Background Circular RNAs (circRNAs) are a class of noncoding RNAs that are involved in the progression of many human cancers. The precise gene locus and the roles of circular RNA from Fibronectin type III domain containing 3B (FNDC3B) in OS and its mechanisms of action have not been fully explored. Materials and methods qRT-qPCR assay was used to determine gene expressions. CCK8 Assay, EdU assay, wound-healing assay, transwell invasion assay and in vivo xenograft assay were used to perform functional investigations. RNA-FISH, immunofluorescence, RIP assay, RNA stability analysis were applied in mechanistic studies. Results We found that circFNDC3B downregulated and FNDC3B mRNA upregulated in OS, and might be potential biomarkers for indicating disease progression and prognosis of OS patients. CircFNDC3B acted as a tumor suppressor gene to restrain OS progression and FNDC3B functioned as an oncogene to promote OS progression in vitro and in vivo. RNA binding protein RNA binding motif protein 47 (RBM47) could bind to the flanking introns of circFNDC3B to facilitate the generation of circFNDC3B, resulting in the reduction of FNDC3B mRNA and the circFNDC3B-FNDC3B mRNA imbalance. CircFNDC3B also inhibited FNDC3B mRNA expression by reducing its stability via competitively binding to Insulin-like growth-factor-2 mRNA binding protein (IGF2BP1). Conclusion This study demonstrated that RBM47 and IGF2BP1 mediated circular FNDC3B/FNDC3B mRNA imbalance was involved in the malignant processes of OS.https://doi.org/10.1186/s12935-023-03175-3Circular RNAOsteosarcomaFNDC3BRBM47IGF2BP1 |
| spellingShingle | Congya Li Linchao Ding Xuyao Wang Peng Shu Xuchao Shi Zhijian Zheng Jian Liu Junlan Zhu A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma Cancer Cell International Circular RNA Osteosarcoma FNDC3B RBM47 IGF2BP1 |
| title | A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma |
| title_full | A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma |
| title_fullStr | A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma |
| title_full_unstemmed | A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma |
| title_short | A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma |
| title_sort | rbm47 and igf2bp1 mediated circular fndc3b fndc3b mrna imbalance is involved in the malignant processes of osteosarcoma |
| topic | Circular RNA Osteosarcoma FNDC3B RBM47 IGF2BP1 |
| url | https://doi.org/10.1186/s12935-023-03175-3 |
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