Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material
Chitosan (CS) and its derivatives show antimicrobial properties. This is of interest in preventing and treating denture stomatitis, which can be caused by fungi. Therefore, the aim of this study was the development of a novel antifungal denture base material by modifying polymethyl methacrylate (PMM...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/24/5899 |
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| author | Katarzyna Walczak Georg Schierz Sabine Basche Carola Petto Klaus Boening Mieszko Wieckiewicz |
| author_facet | Katarzyna Walczak Georg Schierz Sabine Basche Carola Petto Klaus Boening Mieszko Wieckiewicz |
| author_sort | Katarzyna Walczak |
| collection | DOAJ |
| description | Chitosan (CS) and its derivatives show antimicrobial properties. This is of interest in preventing and treating denture stomatitis, which can be caused by fungi. Therefore, the aim of this study was the development of a novel antifungal denture base material by modifying polymethyl methacrylate (PMMA) with CS-salt and characterizing its antifungal and surface properties in vitro. For this purpose, the antifungal effect of chitosan-hydrochloride (CS-HCl) or chitosan-glutamate (CS-G) as solutions in different concentrations was determined. To obtain modified PMMA resin specimens, the CS-salts were added to the PMMA before polymerization. The roughness of these specimens was measured by contact profilometry. For the evaluation of the antifungal properties of the CS-salt modified resins, a <i>C. albicans</i> biofilm assay on the specimens was performed. As solutions, both the CS-G and CS-HCl-salt had an antifungal effect and inhibited <i>C. albicans</i> growth in a dose-dependent manner. In contrast, CS-salt modified PMMA resins showed no significant reduced <i>C. albicans</i> biofilm formation. Furthermore, the addition of CS-salts to PMMA significantly increased the surface roughness of the specimens. This study shows that despite the antifungal effect of CS-salts in solution, a modification of PMMA resin with these CS-salts does not improve the antifungal properties of PMMA denture base material. |
| first_indexed | 2024-03-10T14:06:49Z |
| format | Article |
| id | doaj.art-5c0ab10023f34fbdb05411d2d327f0e5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:06:49Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5c0ab10023f34fbdb05411d2d327f0e52023-11-21T00:35:31ZengMDPI AGMolecules1420-30492020-12-012524589910.3390/molecules25245899Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base MaterialKatarzyna Walczak0Georg Schierz1Sabine Basche2Carola Petto3Klaus Boening4Mieszko Wieckiewicz5Department of Prosthetic Dentistry, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, GermanyDepartment of Prosthetic Dentistry, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, GermanyClinic of Operative and Pediatric Dentistry, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, GermanyDepartment of Oral and Maxillofacial Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, GermanyDepartment of Prosthetic Dentistry, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, GermanyDepartment of Experimental Dentistry, Faculty of Dentistry, Wroclaw Medical University, 26 Krakowska st., 50-425 Wroclaw, PolandChitosan (CS) and its derivatives show antimicrobial properties. This is of interest in preventing and treating denture stomatitis, which can be caused by fungi. Therefore, the aim of this study was the development of a novel antifungal denture base material by modifying polymethyl methacrylate (PMMA) with CS-salt and characterizing its antifungal and surface properties in vitro. For this purpose, the antifungal effect of chitosan-hydrochloride (CS-HCl) or chitosan-glutamate (CS-G) as solutions in different concentrations was determined. To obtain modified PMMA resin specimens, the CS-salts were added to the PMMA before polymerization. The roughness of these specimens was measured by contact profilometry. For the evaluation of the antifungal properties of the CS-salt modified resins, a <i>C. albicans</i> biofilm assay on the specimens was performed. As solutions, both the CS-G and CS-HCl-salt had an antifungal effect and inhibited <i>C. albicans</i> growth in a dose-dependent manner. In contrast, CS-salt modified PMMA resins showed no significant reduced <i>C. albicans</i> biofilm formation. Furthermore, the addition of CS-salts to PMMA significantly increased the surface roughness of the specimens. This study shows that despite the antifungal effect of CS-salts in solution, a modification of PMMA resin with these CS-salts does not improve the antifungal properties of PMMA denture base material.https://www.mdpi.com/1420-3049/25/24/5899chitosanchitosan glutamatechitosan-HClantifungal<i>C. albicans</i>PMMA |
| spellingShingle | Katarzyna Walczak Georg Schierz Sabine Basche Carola Petto Klaus Boening Mieszko Wieckiewicz Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material Molecules chitosan chitosan glutamate chitosan-HCl antifungal <i>C. albicans</i> PMMA |
| title | Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material |
| title_full | Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material |
| title_fullStr | Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material |
| title_full_unstemmed | Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material |
| title_short | Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material |
| title_sort | antifungal and surface properties of chitosan salts modified pmma denture base material |
| topic | chitosan chitosan glutamate chitosan-HCl antifungal <i>C. albicans</i> PMMA |
| url | https://www.mdpi.com/1420-3049/25/24/5899 |
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