Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells
Stem cells from dental tissues have been isolated and established for tooth regenerative applications. However, basic characterization on their biological properties still needs to be investigated before employing them for effective clinical trials. In this study, we compared the telomere length, re...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2011-12-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368911X565001 |
| _version_ | 1818149104631414784 |
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| author | Byeong-Gyun Jeon Eun-Ju Kang B. Mohana Kumar Geun-Ho Maeng Sun-A Ock Dae-Oh Kwack Bong-Wook Park Gyu-Jin Rho D.V.M., Ph.D. |
| author_facet | Byeong-Gyun Jeon Eun-Ju Kang B. Mohana Kumar Geun-Ho Maeng Sun-A Ock Dae-Oh Kwack Bong-Wook Park Gyu-Jin Rho D.V.M., Ph.D. |
| author_sort | Byeong-Gyun Jeon |
| collection | DOAJ |
| description | Stem cells from dental tissues have been isolated and established for tooth regenerative applications. However, basic characterization on their biological properties still needs to be investigated before employing them for effective clinical trials. In this study, we compared the telomere length, relative telomerase activity (RTA), and relative reverse transcriptase activity (RRA) as well as the surface antigen profile and mesenchymal differentiation ability in human dental papilla stem cells (DPaSCs), dental pulp stem cells (DPuSCs), and dental follicle stem cells (DFSCs) with mesenchymal stem cells (MSCs) derived from bone marrow. Dental stem cells (DSCs) were strongly positive for cell surface markers, such as CD44 and CD90. However, slightly lower expression of CD105 was observed in DPaSCs and DPuSCs compared to DFSCs and MSCs. Following specific induction, DPaSCs, DFSCs, and MSCs were successfully differentiated into adipocytes and osteocytes. However, DPuSCS, in particular, were able to differentiate into adipocytes but failed to induce into osteogenic differentiation. Further, all DSCs, MSCs, and MRC-5 fibroblasts as control were investigated for telomere length by nonradioactive chemiluminescent assay, RTA by relative-quantitative telomerase repeat amplification protocol (RQ-TRAP), and RRA by PCR-based assay. Mean telomere lengths in DPaSCs, DPuSCs, DFSCs, and MSCs was ~11 kb, and the values did not differ significantly ( p < 0.05) among the cells analyzed. RTA levels in DPaSCs were significantly ( p < 0.05) higher than in MSCs, DPuSCs, DFSCs, and MRC-5 fibroblasts and among DSCs, DFSCs showed a significantly ( p < 0.05) lower RTA. Moreover, RRA levels were significantly ( p < 0.05) higher in DPaSCs, DPuSCs, and MSCs than in DFSCs. Based on these observations, we conclude that among DSCs, DPaSCs possessed ideal characteristics on telomere length, telomerase activity and reverse transcriptase (RTase) activity, and may serve as suitable alternative candidates for regenerative medicine. |
| first_indexed | 2024-12-11T13:01:44Z |
| format | Article |
| id | doaj.art-5c15ce824971474787e7cd99620db232 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T13:01:44Z |
| publishDate | 2011-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-5c15ce824971474787e7cd99620db2322022-12-22T01:06:27ZengSAGE PublishingCell Transplantation0963-68971555-38922011-12-012010.3727/096368911X565001Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem CellsByeong-Gyun Jeon0Eun-Ju Kang1B. Mohana Kumar2Geun-Ho Maeng3Sun-A Ock4Dae-Oh Kwack5Bong-Wook Park6Gyu-Jin Rho D.V.M., Ph.D.7OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaOBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaOBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaOBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaOBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of KoreaDepartment of Biology Education, College of Education, Gyeongsang National University, Jinju, Republic of KoreaDepartment of Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of KoreaResearch Institute of Life Science, Gyeongsang National University, Jinju, Republic of KoreaStem cells from dental tissues have been isolated and established for tooth regenerative applications. However, basic characterization on their biological properties still needs to be investigated before employing them for effective clinical trials. In this study, we compared the telomere length, relative telomerase activity (RTA), and relative reverse transcriptase activity (RRA) as well as the surface antigen profile and mesenchymal differentiation ability in human dental papilla stem cells (DPaSCs), dental pulp stem cells (DPuSCs), and dental follicle stem cells (DFSCs) with mesenchymal stem cells (MSCs) derived from bone marrow. Dental stem cells (DSCs) were strongly positive for cell surface markers, such as CD44 and CD90. However, slightly lower expression of CD105 was observed in DPaSCs and DPuSCs compared to DFSCs and MSCs. Following specific induction, DPaSCs, DFSCs, and MSCs were successfully differentiated into adipocytes and osteocytes. However, DPuSCS, in particular, were able to differentiate into adipocytes but failed to induce into osteogenic differentiation. Further, all DSCs, MSCs, and MRC-5 fibroblasts as control were investigated for telomere length by nonradioactive chemiluminescent assay, RTA by relative-quantitative telomerase repeat amplification protocol (RQ-TRAP), and RRA by PCR-based assay. Mean telomere lengths in DPaSCs, DPuSCs, DFSCs, and MSCs was ~11 kb, and the values did not differ significantly ( p < 0.05) among the cells analyzed. RTA levels in DPaSCs were significantly ( p < 0.05) higher than in MSCs, DPuSCs, DFSCs, and MRC-5 fibroblasts and among DSCs, DFSCs showed a significantly ( p < 0.05) lower RTA. Moreover, RRA levels were significantly ( p < 0.05) higher in DPaSCs, DPuSCs, and MSCs than in DFSCs. Based on these observations, we conclude that among DSCs, DPaSCs possessed ideal characteristics on telomere length, telomerase activity and reverse transcriptase (RTase) activity, and may serve as suitable alternative candidates for regenerative medicine.https://doi.org/10.3727/096368911X565001 |
| spellingShingle | Byeong-Gyun Jeon Eun-Ju Kang B. Mohana Kumar Geun-Ho Maeng Sun-A Ock Dae-Oh Kwack Bong-Wook Park Gyu-Jin Rho D.V.M., Ph.D. Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells Cell Transplantation |
| title | Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells |
| title_full | Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells |
| title_fullStr | Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells |
| title_full_unstemmed | Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells |
| title_short | Comparative Analysis of Telomere Length, Telomerase and Reverse Transcriptase Activity in Human Dental Stem Cells |
| title_sort | comparative analysis of telomere length telomerase and reverse transcriptase activity in human dental stem cells |
| url | https://doi.org/10.3727/096368911X565001 |
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