Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells
Sertoli cells (SCs) in the mammalian testes are well known as supporting cells of spermatogenesis, but have recently become an attractive source of cell therapy because of their capacity for immune modulation and trophic effects. In order to increase their applicable efficacy, we demonstrate a novel...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689718797053 |
| _version_ | 1818275487375425536 |
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| author | Dong-Won Seol Seah Park Eun Young Shin Jae Ho Chang Dong Ryul Lee |
| author_facet | Dong-Won Seol Seah Park Eun Young Shin Jae Ho Chang Dong Ryul Lee |
| author_sort | Dong-Won Seol |
| collection | DOAJ |
| description | Sertoli cells (SCs) in the mammalian testes are well known as supporting cells of spermatogenesis, but have recently become an attractive source of cell therapy because of their capacity for immune modulation and trophic effects. In order to increase their applicable efficacy, we demonstrate a novel differentiation method for mouse embryonic stem cell (ESC)-derived Sertoli-like cells (SLCs) via the intermediate mesoderm (IM). We show that IM derived from an induction of 6 days expressed markers such as Wt1 , Lhx1 , Pax2 and Osr1, and that a sequential induction of 6 days resulted in ESC-SLCs. The SLCs expressed their marker genes ( Sf1 , Sox9 , Gata4 , Wt1 , Fshr and Scf ), but the pluripotency-marker gene Oct4 was decreased. After sorting by FSHR expression, high-purity (> 90%) SLCs were collected that showed distinct characteristics of SCs such as high phagocytic and immune modulation activities as well as the expression of immune-related genes. In addition, when transplanted into the seminiferous tubule of busulfan-treated mice, SLCs re-located and were maintained in the basal region of the tubule. These results demonstrated that our robust sequential differentiation system produced functional SLCs from mouse ESCs in vitro . |
| first_indexed | 2024-12-12T22:30:32Z |
| format | Article |
| id | doaj.art-5c15fef20ad644a68092be9f4e84d40c |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T22:30:32Z |
| publishDate | 2018-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-5c15fef20ad644a68092be9f4e84d40c2022-12-22T00:09:37ZengSAGE PublishingCell Transplantation0963-68971555-38922018-10-012710.1177/0963689718797053Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem CellsDong-Won Seol0Seah Park1Eun Young Shin2Jae Ho Chang3Dong Ryul Lee4 Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-do, Korea Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-do, Korea Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-do, Korea Department of Bio-Convergence, Underwood International College, Yonsei University, Seoul, Korea Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-do, KoreaSertoli cells (SCs) in the mammalian testes are well known as supporting cells of spermatogenesis, but have recently become an attractive source of cell therapy because of their capacity for immune modulation and trophic effects. In order to increase their applicable efficacy, we demonstrate a novel differentiation method for mouse embryonic stem cell (ESC)-derived Sertoli-like cells (SLCs) via the intermediate mesoderm (IM). We show that IM derived from an induction of 6 days expressed markers such as Wt1 , Lhx1 , Pax2 and Osr1, and that a sequential induction of 6 days resulted in ESC-SLCs. The SLCs expressed their marker genes ( Sf1 , Sox9 , Gata4 , Wt1 , Fshr and Scf ), but the pluripotency-marker gene Oct4 was decreased. After sorting by FSHR expression, high-purity (> 90%) SLCs were collected that showed distinct characteristics of SCs such as high phagocytic and immune modulation activities as well as the expression of immune-related genes. In addition, when transplanted into the seminiferous tubule of busulfan-treated mice, SLCs re-located and were maintained in the basal region of the tubule. These results demonstrated that our robust sequential differentiation system produced functional SLCs from mouse ESCs in vitro .https://doi.org/10.1177/0963689718797053 |
| spellingShingle | Dong-Won Seol Seah Park Eun Young Shin Jae Ho Chang Dong Ryul Lee Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells Cell Transplantation |
| title | Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells |
| title_full | Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells |
| title_fullStr | Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells |
| title_full_unstemmed | Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells |
| title_short | Derivation of Functional Sertoli-Like Cells from Mouse Embryonic Stem Cells |
| title_sort | derivation of functional sertoli like cells from mouse embryonic stem cells |
| url | https://doi.org/10.1177/0963689718797053 |
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