The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation
Background: To investigate the expression of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in renal ischemia-reperfusion injury and explore its role in acute kidney injury. Methods: 18 mice were randomly divided into a sham operation group (Sham) and an ischemia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-10-01
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| Series: | Renal Failure |
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| Online Access: | http://dx.doi.org/10.1080/0886022X.2018.1487863 |
| _version_ | 1818379840501317632 |
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| author | Hongyan Tian Ming Wu Peihui Zhou Chuiguo Huang Chaoyang Ye Li Wang |
| author_facet | Hongyan Tian Ming Wu Peihui Zhou Chuiguo Huang Chaoyang Ye Li Wang |
| author_sort | Hongyan Tian |
| collection | DOAJ |
| description | Background: To investigate the expression of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in renal ischemia-reperfusion injury and explore its role in acute kidney injury. Methods: 18 mice were randomly divided into a sham operation group (Sham) and an ischemia-reperfusion group (IR) in which animals were sacrificed at 6 h or 12 h after surgery. The kidneys were harvested to measure the expression of MALAT1 mRNA. HK2 cells were treated with cobalt chloride (CoCl2) to mimic hypoxia or transfected with siRNA to knockdown MALAT1 before CoCl2 treatment. After that, MALAT1 was analyzed by RT-PCR (reverse transcription-polymerase chain reaction). HIF-1ɑ (hypoxia-inducible factor-1 alpha) and NF-κB (nuclear factor-kappa B) was measured by Western blot. The concentrations of IL-6 (interleukin-6) and TNF-ɑ (tumor necrosis factor-alpha) in the media were detected by ELISA (enzyme-linked immunosorbent assay). Results: The expression of MALAT1 in the IR (6 h/12 h) group was significantly higher than that in the sham group. In HK2 cells, MALAT1 was significantly increased at 1 h, 3 h, and 6 h after CoCl2 treatment but had reduced to the basal level at 12 h and 24 h. Knockdown of MALAT1 by siRNA significantly up-regulated the expression of HIF-1ɑ and NF-κB proteins in CoCl2-treated HK2 cells. In addition, the concentrations of IL-6 and TNF-ɑ were increased by MALAT1 siRNA transfection in CoCl2-treated HK2 cells. Conclusion: The expression of MALAT1 is increased in renal ischemia-reperfusion injury. It is likely that MALAT1 inhibits the hypoxia-induced inflammatory response through the NF-κB pathway. |
| first_indexed | 2024-12-14T02:09:11Z |
| format | Article |
| id | doaj.art-5c1705c286e649a7b239c0042ab507ee |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-12-14T02:09:11Z |
| publishDate | 2018-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-5c1705c286e649a7b239c0042ab507ee2022-12-21T23:20:49ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492018-10-0140152753310.1080/0886022X.2018.14878631487863The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammationHongyan Tian0Ming Wu1Peihui Zhou2Chuiguo Huang3Chaoyang Ye4Li Wang5The Ninth People’s Hospital, Shanghai Jiaotong University School of MedicineShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineThe Ninth People’s Hospital, Shanghai Jiaotong University School of Medicinethe second affiliated hospital of Zhengzhou UniversityShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineThe Ninth People’s Hospital, Shanghai Jiaotong University School of MedicineBackground: To investigate the expression of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in renal ischemia-reperfusion injury and explore its role in acute kidney injury. Methods: 18 mice were randomly divided into a sham operation group (Sham) and an ischemia-reperfusion group (IR) in which animals were sacrificed at 6 h or 12 h after surgery. The kidneys were harvested to measure the expression of MALAT1 mRNA. HK2 cells were treated with cobalt chloride (CoCl2) to mimic hypoxia or transfected with siRNA to knockdown MALAT1 before CoCl2 treatment. After that, MALAT1 was analyzed by RT-PCR (reverse transcription-polymerase chain reaction). HIF-1ɑ (hypoxia-inducible factor-1 alpha) and NF-κB (nuclear factor-kappa B) was measured by Western blot. The concentrations of IL-6 (interleukin-6) and TNF-ɑ (tumor necrosis factor-alpha) in the media were detected by ELISA (enzyme-linked immunosorbent assay). Results: The expression of MALAT1 in the IR (6 h/12 h) group was significantly higher than that in the sham group. In HK2 cells, MALAT1 was significantly increased at 1 h, 3 h, and 6 h after CoCl2 treatment but had reduced to the basal level at 12 h and 24 h. Knockdown of MALAT1 by siRNA significantly up-regulated the expression of HIF-1ɑ and NF-κB proteins in CoCl2-treated HK2 cells. In addition, the concentrations of IL-6 and TNF-ɑ were increased by MALAT1 siRNA transfection in CoCl2-treated HK2 cells. Conclusion: The expression of MALAT1 is increased in renal ischemia-reperfusion injury. It is likely that MALAT1 inhibits the hypoxia-induced inflammatory response through the NF-κB pathway.http://dx.doi.org/10.1080/0886022X.2018.1487863Acute kidney injuryischemia/reperfusioninflammationlong non-coding RNAMALAT1 |
| spellingShingle | Hongyan Tian Ming Wu Peihui Zhou Chuiguo Huang Chaoyang Ye Li Wang The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation Renal Failure Acute kidney injury ischemia/reperfusion inflammation long non-coding RNA MALAT1 |
| title | The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation |
| title_full | The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation |
| title_fullStr | The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation |
| title_full_unstemmed | The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation |
| title_short | The long non-coding RNA MALAT1 is increased in renal ischemia-reperfusion injury and inhibits hypoxia-induced inflammation |
| title_sort | long non coding rna malat1 is increased in renal ischemia reperfusion injury and inhibits hypoxia induced inflammation |
| topic | Acute kidney injury ischemia/reperfusion inflammation long non-coding RNA MALAT1 |
| url | http://dx.doi.org/10.1080/0886022X.2018.1487863 |
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