The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
Introduction The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of UCA1/ microRNA-18a signaling pathway in the control of PCOS. Material and methods Real-time PCR was performed to compare the l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2021-03-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/The-UCA1-and-microRNA-18a-signaling-pathway-mediates-the-irisin-lowering-effect-of,103379,0,2.html |
| _version_ | 1797998106604732416 |
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| author | Wei Wang Tian Hua Xiaodong Li Xinxian Zhang Wei Hao |
| author_facet | Wei Wang Tian Hua Xiaodong Li Xinxian Zhang Wei Hao |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Introduction
The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of UCA1/ microRNA-18a signaling pathway in the control of PCOS.
Material and methods
Real-time PCR was performed to compare the level of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western-blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin.
Results
AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bound to irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, the treatment with met at the increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose dependent manner.
Conclusions
In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway. |
| first_indexed | 2024-04-11T10:43:15Z |
| format | Article |
| id | doaj.art-5c19e12b878c485fb7500fc608090cd9 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-11T10:43:15Z |
| publishDate | 2021-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-5c19e12b878c485fb7500fc608090cd92022-12-22T04:29:08ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-03-0118248949810.5114/aoms/103379103379The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndromeWei Wang0Tian Hua1Xiaodong Li2Xinxian Zhang3Wei Hao4Department of Reproduction, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, ChinaDepartment of Gynecology, Xingtai People’s Hospital Affiliated to Hebei Medial University, Xingtai, Hebei Province, ChinaDepartment of Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, ChinaDepartment of Reproduction, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, ChinaDepartment of Reproduction, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, ChinaIntroduction The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of UCA1/ microRNA-18a signaling pathway in the control of PCOS. Material and methods Real-time PCR was performed to compare the level of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western-blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin. Results AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bound to irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, the treatment with met at the increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose dependent manner. Conclusions In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway.https://www.archivesofmedicalscience.com/The-UCA1-and-microRNA-18a-signaling-pathway-mediates-the-irisin-lowering-effect-of,103379,0,2.htmluca1mir-18airisinmetpolycystic ovary syndrome |
| spellingShingle | Wei Wang Tian Hua Xiaodong Li Xinxian Zhang Wei Hao The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome Archives of Medical Science uca1 mir-18a irisin met polycystic ovary syndrome |
| title | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
| title_full | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
| title_fullStr | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
| title_full_unstemmed | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
| title_short | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
| title_sort | uca1 and microrna 18a signaling pathway mediates the irisin lowering effect of metformin in the management of polycystic ovary syndrome |
| topic | uca1 mir-18a irisin met polycystic ovary syndrome |
| url | https://www.archivesofmedicalscience.com/The-UCA1-and-microRNA-18a-signaling-pathway-mediates-the-irisin-lowering-effect-of,103379,0,2.html |
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