Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity
Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its gro...
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| Format: | Article |
| Language: | English |
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Association of Support to Oral Health Research (APESB)
2021-11-01
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| Series: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
| Subjects: | |
| Online Access: | https://revista.uepb.edu.br/PBOCI/article/view/611 |
| _version_ | 1818775786065231872 |
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| author | Dharli Syafriza Heriandi Sutadi Ameta Primasari Yahwardiah Siregar |
| author_facet | Dharli Syafriza Heriandi Sutadi Ameta Primasari Yahwardiah Siregar |
| author_sort | Dharli Syafriza |
| collection | DOAJ |
| description | Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva. |
| first_indexed | 2024-12-18T11:02:34Z |
| format | Article |
| id | doaj.art-5c19fac7375f4480944274e07f385b3e |
| institution | Directory Open Access Journal |
| issn | 1519-0501 1983-4632 |
| language | English |
| last_indexed | 2024-12-18T11:02:34Z |
| publishDate | 2021-11-01 |
| publisher | Association of Support to Oral Health Research (APESB) |
| record_format | Article |
| series | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
| spelling | doaj.art-5c19fac7375f4480944274e07f385b3e2022-12-21T21:10:11ZengAssociation of Support to Oral Health Research (APESB)Pesquisa Brasileira em Odontopediatria e Clínica Integrada1519-05011983-46322021-11-0121Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and ViscosityDharli SyafrizaHeriandi SutadiAmeta PrimasariYahwardiah SiregarObjective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva.https://revista.uepb.edu.br/PBOCI/article/view/611SalivaHistatinsViridans StreptococciBiofilmsHydrogen-Ion Concentration |
| spellingShingle | Dharli Syafriza Heriandi Sutadi Ameta Primasari Yahwardiah Siregar Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity Pesquisa Brasileira em Odontopediatria e Clínica Integrada Saliva Histatins Viridans Streptococci Biofilms Hydrogen-Ion Concentration |
| title | Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity |
| title_full | Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity |
| title_fullStr | Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity |
| title_full_unstemmed | Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity |
| title_short | Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity |
| title_sort | spectrophotometric analysis of streptococcus mutans growth and biofilm formation in saliva and histatin 5 relate to ph and viscosity |
| topic | Saliva Histatins Viridans Streptococci Biofilms Hydrogen-Ion Concentration |
| url | https://revista.uepb.edu.br/PBOCI/article/view/611 |
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