Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease
Dietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP supplementation reduces acute mucosal inflammation, as well as chronic inflammation associated with aging. The aim of this study was to analyze if SDP supplementation...
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/18/6760 |
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| author | Lluïsa Miró Concepció Amat Cristina Rosell-Cardona Joy M. Campbell Javier Polo Anna Pérez-Bosque Miquel Moretó |
| author_facet | Lluïsa Miró Concepció Amat Cristina Rosell-Cardona Joy M. Campbell Javier Polo Anna Pérez-Bosque Miquel Moretó |
| author_sort | Lluïsa Miró |
| collection | DOAJ |
| description | Dietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP supplementation reduces acute mucosal inflammation, as well as chronic inflammation associated with aging. The aim of this study was to analyze if SDP supplementation could ameliorate colitis in a genetic mouse model of inflammatory bowel disease (IBD). Wild-type mice and <i>Mdr1a</i> knockout (KO) mice were administered a control diet or an SDP-supplemented diet from day 21 (weaning) until day 56. The histopathological index, epithelial barrier, and intestinal immune system were analyzed in the colonic mucosa. KO mice had higher epithelial permeability, increased <i>Muc1</i> and <i>Muc4</i> expression, and lower abundance of E-cadherin and <i>Muc2</i> (all <i>p</i> < 0.001). SDP prevented these effects (all <i>p</i> < 0.05) and decreased the colonic inflammation observed in KO mice, reducing neutrophil and monocyte infiltration and activation and the percentage of activated T helper lymphocytes in the colonic mucosa (all <i>p</i> < 0.05). SDP also diminished proinflammatory cytokine expression and increased the anti-inflammatory IL-10 concentration in the colonic mucosa (all <i>p</i> < 0.05). In conclusion, dietary supplementation with SDP enhances colon barrier function and reduces mucosal inflammation in a mouse model of IBD. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:19:32Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5c1a2aad4e31458bb7b0f24993d79af42023-11-20T13:47:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118676010.3390/ijms21186760Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel DiseaseLluïsa Miró0Concepció Amat1Cristina Rosell-Cardona2Joy M. Campbell3Javier Polo4Anna Pérez-Bosque5Miquel Moretó6Dept. of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, and Nutrition and Food Safety Research Institute, Universitat de Barcelona (UB), 08028 Barcelona, SpainDept. of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, and Nutrition and Food Safety Research Institute, Universitat de Barcelona (UB), 08028 Barcelona, SpainDept. of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, and Nutrition and Food Safety Research Institute, Universitat de Barcelona (UB), 08028 Barcelona, SpainAPC LLC, 2425 SE Oak Tree Court, Ankeny, IA 50021, USAAPC-Europe S.L.U., 08403 Granollers, SpainDept. of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, and Nutrition and Food Safety Research Institute, Universitat de Barcelona (UB), 08028 Barcelona, SpainDept. of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, and Nutrition and Food Safety Research Institute, Universitat de Barcelona (UB), 08028 Barcelona, SpainDietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP supplementation reduces acute mucosal inflammation, as well as chronic inflammation associated with aging. The aim of this study was to analyze if SDP supplementation could ameliorate colitis in a genetic mouse model of inflammatory bowel disease (IBD). Wild-type mice and <i>Mdr1a</i> knockout (KO) mice were administered a control diet or an SDP-supplemented diet from day 21 (weaning) until day 56. The histopathological index, epithelial barrier, and intestinal immune system were analyzed in the colonic mucosa. KO mice had higher epithelial permeability, increased <i>Muc1</i> and <i>Muc4</i> expression, and lower abundance of E-cadherin and <i>Muc2</i> (all <i>p</i> < 0.001). SDP prevented these effects (all <i>p</i> < 0.05) and decreased the colonic inflammation observed in KO mice, reducing neutrophil and monocyte infiltration and activation and the percentage of activated T helper lymphocytes in the colonic mucosa (all <i>p</i> < 0.05). SDP also diminished proinflammatory cytokine expression and increased the anti-inflammatory IL-10 concentration in the colonic mucosa (all <i>p</i> < 0.05). In conclusion, dietary supplementation with SDP enhances colon barrier function and reduces mucosal inflammation in a mouse model of IBD.https://www.mdpi.com/1422-0067/21/18/6760spray-dried plasmaanti-inflammatorycolitisbarrier function |
| spellingShingle | Lluïsa Miró Concepció Amat Cristina Rosell-Cardona Joy M. Campbell Javier Polo Anna Pérez-Bosque Miquel Moretó Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease International Journal of Molecular Sciences spray-dried plasma anti-inflammatory colitis barrier function |
| title | Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease |
| title_full | Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease |
| title_fullStr | Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease |
| title_full_unstemmed | Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease |
| title_short | Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease |
| title_sort | dietary supplementation with spray dried porcine plasma attenuates colon inflammation in a genetic mouse model of inflammatory bowel disease |
| topic | spray-dried plasma anti-inflammatory colitis barrier function |
| url | https://www.mdpi.com/1422-0067/21/18/6760 |
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