Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy
Age-related changes in bone tissue have always been an important part of bone research, and age estimation is also of great significance in forensic work. In our study, FTIR and Raman microspectroscopy were combined to explore the structural and chronological age-related changes in the occipital bon...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2022/1729131 |
| _version_ | 1798036097580662784 |
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| author | Kai Yu Hongli Xiong Xin Wei Hao Wu Bo Zhang Gongji Wang Xiaorong Yang Zhenyuan Wang |
| author_facet | Kai Yu Hongli Xiong Xin Wei Hao Wu Bo Zhang Gongji Wang Xiaorong Yang Zhenyuan Wang |
| author_sort | Kai Yu |
| collection | DOAJ |
| description | Age-related changes in bone tissue have always been an important part of bone research, and age estimation is also of great significance in forensic work. In our study, FTIR and Raman microspectroscopy were combined to explore the structural and chronological age-related changes in the occipital bones of 40 male donors. The FTIR micro-ATR mode not only achieves the comparison of FTIR and Raman efficiency but also provides a new pattern for the joint detection of FTIR and Raman in hard tissue. Statistical analysis and PCA results revealed that the structure had little effect on the FTIR and Raman results. The FTIR and Raman mineral/matrix ratio, carbonate/phosphate ratio, crystallinity, and collagen maturity of the whole showed an increasing trend during maturation, and a significant correlation was found between FTIR and Raman by comparing four outcomes. Furthermore, the results indicated that the cutoff point of the change in the relative proportion of organic matrix and inorganic minerals in males was between 19 and 35 years old, and the changes in the relative proportion of organic matrix and inorganic minerals may play a key role in age estimation. Ultimately, we established age estimation regression models. The FTIR GA-PLS regression model has the best performance and is more suitable for our experiment (RMSECV = 10.405, RMSEP = 9.2654, R2CV = 0.814, and R2Pred = 0.828). Overall, FTIR and Raman combined with chemometrics are an ideal method to estimate chronological age based on age-dependent component changes in male occipital bones. Our experiment provides a proof of concept and potential experimental method for chronological age estimation. |
| first_indexed | 2024-04-11T21:07:52Z |
| format | Article |
| id | doaj.art-5c1c69cd313a461ba3d52998f2a15266 |
| institution | Directory Open Access Journal |
| issn | 1687-479X |
| language | English |
| last_indexed | 2024-04-11T21:07:52Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj.art-5c1c69cd313a461ba3d52998f2a152662022-12-22T04:03:09ZengHindawi LimitedBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/1729131Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman MicrospectroscopyKai Yu0Hongli Xiong1Xin Wei2Hao Wu3Bo Zhang4Gongji Wang5Xiaorong Yang6Zhenyuan Wang7Department of Forensic PathologyDepartment of Forensic MedicineDepartment of Forensic PathologyDepartment of Forensic PathologyXi’an Jiaotong UniversityDepartment of Forensic PathologyDepartment of Forensic MedicineDepartment of Forensic PathologyAge-related changes in bone tissue have always been an important part of bone research, and age estimation is also of great significance in forensic work. In our study, FTIR and Raman microspectroscopy were combined to explore the structural and chronological age-related changes in the occipital bones of 40 male donors. The FTIR micro-ATR mode not only achieves the comparison of FTIR and Raman efficiency but also provides a new pattern for the joint detection of FTIR and Raman in hard tissue. Statistical analysis and PCA results revealed that the structure had little effect on the FTIR and Raman results. The FTIR and Raman mineral/matrix ratio, carbonate/phosphate ratio, crystallinity, and collagen maturity of the whole showed an increasing trend during maturation, and a significant correlation was found between FTIR and Raman by comparing four outcomes. Furthermore, the results indicated that the cutoff point of the change in the relative proportion of organic matrix and inorganic minerals in males was between 19 and 35 years old, and the changes in the relative proportion of organic matrix and inorganic minerals may play a key role in age estimation. Ultimately, we established age estimation regression models. The FTIR GA-PLS regression model has the best performance and is more suitable for our experiment (RMSECV = 10.405, RMSEP = 9.2654, R2CV = 0.814, and R2Pred = 0.828). Overall, FTIR and Raman combined with chemometrics are an ideal method to estimate chronological age based on age-dependent component changes in male occipital bones. Our experiment provides a proof of concept and potential experimental method for chronological age estimation.http://dx.doi.org/10.1155/2022/1729131 |
| spellingShingle | Kai Yu Hongli Xiong Xin Wei Hao Wu Bo Zhang Gongji Wang Xiaorong Yang Zhenyuan Wang Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy Bioinorganic Chemistry and Applications |
| title | Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy |
| title_full | Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy |
| title_fullStr | Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy |
| title_full_unstemmed | Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy |
| title_short | Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy |
| title_sort | chronological age estimation of male occipital bone based on ftir and raman microspectroscopy |
| url | http://dx.doi.org/10.1155/2022/1729131 |
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