MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS
Dendritic cells function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. Although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies v...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2003-09-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/46 |
| _version_ | 1819008251721678848 |
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| author | A.H. Zarnani S.M. Moazzeni 1 R Shokri Modzdeh Salehnia A.A. Bayat S. A.R. Mahmoodi Pouneh Dokouhaki and M.Jeddi-Tehrani |
| author_facet | A.H. Zarnani S.M. Moazzeni 1 R Shokri Modzdeh Salehnia A.A. Bayat S. A.R. Mahmoodi Pouneh Dokouhaki and M.Jeddi-Tehrani |
| author_sort | A.H. Zarnani |
| collection | DOAJ |
| description | Dendritic cells function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. Although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies very difficult.
In this study, we purified dendritic cells from mouse spleen by a three-step enrich¬ment method and evaluated morphological and cytochemical characteristics of isolated cells.
We showed that isolated dendritic cells from mouse spleen had all lobulated nuclei with multiple cytoplasmic projections and their morphological features changed after an overnight incubation. It was also shown that typical dendritic cells lacked both Myeloperoxidase (MPO) andNon Specific Esterase (NSE) activity.
In conclusion, for reaching a reasonable purity in isolation of dendritic cells from lymphoid tissues, many enrichment steps should be taken, and for determining the pu¬rity of isolated cells, we recommend that a combination of morphological and cytochemi¬cal studies be used. |
| first_indexed | 2024-12-21T00:37:30Z |
| format | Article |
| id | doaj.art-5c1d80486f1d42dbb5084ce440baf1b0 |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-21T00:37:30Z |
| publishDate | 2003-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-5c1d80486f1d42dbb5084ce440baf1b02022-12-21T19:21:45ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492003-09-012346MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS A.H. Zarnani0 S.M. Moazzeni1 1 R Shokri2 Modzdeh Salehnia3 A.A. Bayat4 S. A.R. Mahmoodi5 Pouneh Dokouhaki6 and M.Jeddi-Tehrani7 Dendritic cells function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. Although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies very difficult. In this study, we purified dendritic cells from mouse spleen by a three-step enrich¬ment method and evaluated morphological and cytochemical characteristics of isolated cells. We showed that isolated dendritic cells from mouse spleen had all lobulated nuclei with multiple cytoplasmic projections and their morphological features changed after an overnight incubation. It was also shown that typical dendritic cells lacked both Myeloperoxidase (MPO) andNon Specific Esterase (NSE) activity. In conclusion, for reaching a reasonable purity in isolation of dendritic cells from lymphoid tissues, many enrichment steps should be taken, and for determining the pu¬rity of isolated cells, we recommend that a combination of morphological and cytochemi¬cal studies be used.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/46CytochemistryMyeloperoxidaseNonspe¬cific Esterase |
| spellingShingle | A.H. Zarnani S.M. Moazzeni 1 R Shokri Modzdeh Salehnia A.A. Bayat S. A.R. Mahmoodi Pouneh Dokouhaki and M.Jeddi-Tehrani MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS Iranian Journal of Allergy, Asthma and Immunology Cytochemistry Myeloperoxidase Nonspe¬cific Esterase |
| title | MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS |
| title_full | MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS |
| title_fullStr | MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS |
| title_full_unstemmed | MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS |
| title_short | MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS |
| title_sort | morphological and cytochemical characteristics of purified murine splenic dendritic cells |
| topic | Cytochemistry Myeloperoxidase Nonspe¬cific Esterase |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/46 |
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