Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior.
Mouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct beha...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6072066?pdf=render |
| _version_ | 1828154933350760448 |
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| author | Pan Zhang Justin S Rhodes Theodore Garland Sam D Perez Bruce R Southey Sandra L Rodriguez-Zas |
| author_facet | Pan Zhang Justin S Rhodes Theodore Garland Sam D Perez Bruce R Southey Sandra L Rodriguez-Zas |
| author_sort | Pan Zhang |
| collection | DOAJ |
| description | Mouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct behavior components necessitates the simultaneous study of these regions. The goals of this study were to identify brain-region dependent and independent gene expression patterns, regulators, and networks associated with increased voluntary wheel-running behavior. The cerebellum and striatum from a high voluntary running line and a non-selected control line were compared. Neuropeptide genes annotated to reward-dependent processes including neuropeptide S receptor 1 (Npsr1), neuropeptide Y (Npy), and proprotein convertase subtilisin/kexin type 9 (Pcsk9), and genes implicated in motor coordination including vitamin D receptor (Vdr) and keratin, type I cytoskeletal 25 (Krt25) were among the genes exhibiting activity line-by-region interaction effects. Genes annotated to the Parkinson pathway presented consistent line patterns, albeit at different orders of magnitude between brain regions, suggesting some parallel events in response to selection for high voluntary activity. The comparison of gene networks between brain regions highlighted genes including transcription factor AP-2-delta (Tfap2d), distal-less homeobox 5 gene (Dlx5) and sine oculis homeobox homolog 3 (Six3) that exhibited line differential expression in one brain region and are associated with reward-dependent behaviors. Transcription factors including En2, Stat6 and Eomes predominated among regulators of genes that differed in expression between lines. Results from the simultaneous study of striatum and cerebellum confirm the necessity to study molecular mechanisms associated with voluntary activity and reward-dependent behaviors in consideration of brain region dependencies. |
| first_indexed | 2024-04-11T22:46:08Z |
| format | Article |
| id | doaj.art-5c229d53dd7f4958ab0df461e0574a1b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:46:08Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5c229d53dd7f4958ab0df461e0574a1b2022-12-22T03:58:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020177310.1371/journal.pone.0201773Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior.Pan ZhangJustin S RhodesTheodore GarlandSam D PerezBruce R SoutheySandra L Rodriguez-ZasMouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct behavior components necessitates the simultaneous study of these regions. The goals of this study were to identify brain-region dependent and independent gene expression patterns, regulators, and networks associated with increased voluntary wheel-running behavior. The cerebellum and striatum from a high voluntary running line and a non-selected control line were compared. Neuropeptide genes annotated to reward-dependent processes including neuropeptide S receptor 1 (Npsr1), neuropeptide Y (Npy), and proprotein convertase subtilisin/kexin type 9 (Pcsk9), and genes implicated in motor coordination including vitamin D receptor (Vdr) and keratin, type I cytoskeletal 25 (Krt25) were among the genes exhibiting activity line-by-region interaction effects. Genes annotated to the Parkinson pathway presented consistent line patterns, albeit at different orders of magnitude between brain regions, suggesting some parallel events in response to selection for high voluntary activity. The comparison of gene networks between brain regions highlighted genes including transcription factor AP-2-delta (Tfap2d), distal-less homeobox 5 gene (Dlx5) and sine oculis homeobox homolog 3 (Six3) that exhibited line differential expression in one brain region and are associated with reward-dependent behaviors. Transcription factors including En2, Stat6 and Eomes predominated among regulators of genes that differed in expression between lines. Results from the simultaneous study of striatum and cerebellum confirm the necessity to study molecular mechanisms associated with voluntary activity and reward-dependent behaviors in consideration of brain region dependencies.http://europepmc.org/articles/PMC6072066?pdf=render |
| spellingShingle | Pan Zhang Justin S Rhodes Theodore Garland Sam D Perez Bruce R Southey Sandra L Rodriguez-Zas Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. PLoS ONE |
| title | Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. |
| title_full | Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. |
| title_fullStr | Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. |
| title_full_unstemmed | Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. |
| title_short | Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior. |
| title_sort | brain region dependent gene networks associated with selective breeding for increased voluntary wheel running behavior |
| url | http://europepmc.org/articles/PMC6072066?pdf=render |
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