Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking wa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340919304895 |
| _version_ | 1818309666846801920 |
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| author | Innocentia G. Erdogan Lukhanyo Mekuto Seteno K.O. Ntwampe Elvis Fosso-Kankeu Frans B. Waanders |
| author_facet | Innocentia G. Erdogan Lukhanyo Mekuto Seteno K.O. Ntwampe Elvis Fosso-Kankeu Frans B. Waanders |
| author_sort | Innocentia G. Erdogan |
| collection | DOAJ |
| description | The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified. Keywords: Drinking water supply system (DWSS), Metagenomics, O'Kiep, 16S rRNA gene |
| first_indexed | 2024-12-13T07:33:48Z |
| format | Article |
| id | doaj.art-5c25838b3367439c97eb0ede9c423f33 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-13T07:33:48Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-5c25838b3367439c97eb0ede9c423f332022-12-21T23:55:07ZengElsevierData in Brief2352-34092019-08-0125Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)Innocentia G. Erdogan0Lukhanyo Mekuto1Seteno K.O. Ntwampe2Elvis Fosso-Kankeu3Frans B. Waanders4Water Pollution Monitoring and Remediation Initiatives Research Group in the CoE C-based Fuels School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South Africa; Bioresource Engineering Research Group (BioERG), Cape Peninsula University of Technology, Cape Town, South Africa; Department of Chemical Engineering, Cape Peninsula University of Technology, Cape Town, South Africa; Corresponding author. Water Pollution Monitoring and Remediation Initiatives Research Group in the CoE C-based Fuels School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South Africa.Bioresource Engineering Research Group (BioERG), Cape Peninsula University of Technology, Cape Town, South Africa; Department of Chemical Engineering, University of Johannesburg, Johannesburg, 2028, South AfricaBioresource Engineering Research Group (BioERG), Cape Peninsula University of Technology, Cape Town, South Africa; Department of Chemical Engineering, Cape Peninsula University of Technology, Cape Town, South AfricaWater Pollution Monitoring and Remediation Initiatives Research Group in the CoE C-based Fuels School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South AfricaWater Pollution Monitoring and Remediation Initiatives Research Group in the CoE C-based Fuels School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South AfricaThe metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified. Keywords: Drinking water supply system (DWSS), Metagenomics, O'Kiep, 16S rRNA genehttp://www.sciencedirect.com/science/article/pii/S2352340919304895 |
| spellingShingle | Innocentia G. Erdogan Lukhanyo Mekuto Seteno K.O. Ntwampe Elvis Fosso-Kankeu Frans B. Waanders Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) Data in Brief |
| title | Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) |
| title_full | Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) |
| title_fullStr | Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) |
| title_full_unstemmed | Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) |
| title_short | Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) |
| title_sort | metagenomic profiling dataset of bacterial communities of a drinking water supply system dwss in the arid namaqualand region south africa source lower orange river to point of use o kiep |
| url | http://www.sciencedirect.com/science/article/pii/S2352340919304895 |
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