In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models

In recent years, the emerging technology of cold atmospheric pressure plasma (CAP) has grown rapidly along with the many medical applications of cold plasma (e.g., cancer, skin disease, tissue repair, etc.). Plasma-activated liquids (e.g., culture media, water, or normal saline, previously exposed t...

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Main Authors: Miao Qi, Dehui Xu, Shuai Wang, Bing Li, Sansan Peng, Qiaosong Li, Hao Zhang, Runze Fan, Hailan Chen, Michael G. Kong
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Biomedicines
Subjects:
Online Access:https://www.mdpi.com/2227-9059/10/3/528
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author Miao Qi
Dehui Xu
Shuai Wang
Bing Li
Sansan Peng
Qiaosong Li
Hao Zhang
Runze Fan
Hailan Chen
Michael G. Kong
author_facet Miao Qi
Dehui Xu
Shuai Wang
Bing Li
Sansan Peng
Qiaosong Li
Hao Zhang
Runze Fan
Hailan Chen
Michael G. Kong
author_sort Miao Qi
collection DOAJ
description In recent years, the emerging technology of cold atmospheric pressure plasma (CAP) has grown rapidly along with the many medical applications of cold plasma (e.g., cancer, skin disease, tissue repair, etc.). Plasma-activated liquids (e.g., culture media, water, or normal saline, previously exposed to plasma) are being studied as cancer treatments, and due to their advantages, many researchers prefer plasma-activated liquids as an alternative to CAP in the treatment of cancer. In this study, we showed that plasma-activated-saline (PAS) treatment significantly inhibited tumor growth, as compared with saline, in melanoma, and a low-pH environment had little effect on tumor growth in vivo. In addition, based on an ultra-high-performance liquid tandem chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) analysis of tumor cell metabolism, the glycerophospholipid metabolic pathway was the most susceptible metabolic pathway to PAS treatment in melanoma in vitro and in vivo. Furthermore, PAS also inhibited cell proliferation in vivo in oral tongue squamous-cell cancer and non-small-cell lung cancer. There were few toxic side effects in the three animal models, and the treatment was deemed safe to use. In the future, plasma-activated liquids may serve as a potential therapeutic approach in the treatment of cancer.
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spelling doaj.art-5c28220393c84137a848160f50b516272023-11-24T00:31:32ZengMDPI AGBiomedicines2227-90592022-02-0110352810.3390/biomedicines10030528In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal ModelsMiao Qi0Dehui Xu1Shuai Wang2Bing Li3Sansan Peng4Qiaosong Li5Hao Zhang6Runze Fan7Hailan Chen8Michael G. Kong9State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaState Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaThe School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaThe School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaState Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaState Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaState Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaState Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, ChinaFrank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USAFrank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USAIn recent years, the emerging technology of cold atmospheric pressure plasma (CAP) has grown rapidly along with the many medical applications of cold plasma (e.g., cancer, skin disease, tissue repair, etc.). Plasma-activated liquids (e.g., culture media, water, or normal saline, previously exposed to plasma) are being studied as cancer treatments, and due to their advantages, many researchers prefer plasma-activated liquids as an alternative to CAP in the treatment of cancer. In this study, we showed that plasma-activated-saline (PAS) treatment significantly inhibited tumor growth, as compared with saline, in melanoma, and a low-pH environment had little effect on tumor growth in vivo. In addition, based on an ultra-high-performance liquid tandem chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) analysis of tumor cell metabolism, the glycerophospholipid metabolic pathway was the most susceptible metabolic pathway to PAS treatment in melanoma in vitro and in vivo. Furthermore, PAS also inhibited cell proliferation in vivo in oral tongue squamous-cell cancer and non-small-cell lung cancer. There were few toxic side effects in the three animal models, and the treatment was deemed safe to use. In the future, plasma-activated liquids may serve as a potential therapeutic approach in the treatment of cancer.https://www.mdpi.com/2227-9059/10/3/528plasmaCAPPAScancer treatmentmetabolic pathway
spellingShingle Miao Qi
Dehui Xu
Shuai Wang
Bing Li
Sansan Peng
Qiaosong Li
Hao Zhang
Runze Fan
Hailan Chen
Michael G. Kong
In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
Biomedicines
plasma
CAP
PAS
cancer treatment
metabolic pathway
title In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
title_full In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
title_fullStr In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
title_full_unstemmed In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
title_short In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models
title_sort in vivo metabolic analysis of the anticancer effects of plasma activated saline in three tumor animal models
topic plasma
CAP
PAS
cancer treatment
metabolic pathway
url https://www.mdpi.com/2227-9059/10/3/528
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