ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress.
Due to the physicochemical properties of nanoparticles, the use of nanomaterials increases over time in industrial and medical processes. We herein report the negative impact of nanoparticles, using solid growth conditions mimicking a biofilm, on the ability of Bacillus subtilis to fight against a s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0240510 |
| _version_ | 1818575398151127040 |
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| author | Elise Eymard-Vernain Sylvie Luche Thierry Rabilloud Cécile Lelong |
| author_facet | Elise Eymard-Vernain Sylvie Luche Thierry Rabilloud Cécile Lelong |
| author_sort | Elise Eymard-Vernain |
| collection | DOAJ |
| description | Due to the physicochemical properties of nanoparticles, the use of nanomaterials increases over time in industrial and medical processes. We herein report the negative impact of nanoparticles, using solid growth conditions mimicking a biofilm, on the ability of Bacillus subtilis to fight against a stress. Bacteria have been exposed to sublethal doses of nanoparticles corresponding to conditions that bacteria may meet in their natural biotopes, the upper layer of soil or the gut microbiome. The analysis of the proteomic data obtained by shotgun mass spectrometry have shown that several metabolic pathways are affected in response to nanoparticles, n-ZnO or n-TiO2, or zinc salt: the methyglyoxal and thiol metabolisms, the oxidative stress and the stringent responses. Nanoparticles being embedded in the agar medium, these impacts are the consequence of a physiological adaptation rather than a physical cell injury. Overall, these results show that nanoparticles, by altering bacterial physiology and especially the ability to resist to a stress, may have profound influences on a "good bacteria", Bacillus subtilis, in its natural biotope and moreover, on the global equilibrium of this biotope. |
| first_indexed | 2024-12-15T00:39:45Z |
| format | Article |
| id | doaj.art-5c293c38b70846a199b74adcb22c4f95 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-15T00:39:45Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5c293c38b70846a199b74adcb22c4f952022-12-21T22:41:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e024051010.1371/journal.pone.0240510ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress.Elise Eymard-VernainSylvie LucheThierry RabilloudCécile LelongDue to the physicochemical properties of nanoparticles, the use of nanomaterials increases over time in industrial and medical processes. We herein report the negative impact of nanoparticles, using solid growth conditions mimicking a biofilm, on the ability of Bacillus subtilis to fight against a stress. Bacteria have been exposed to sublethal doses of nanoparticles corresponding to conditions that bacteria may meet in their natural biotopes, the upper layer of soil or the gut microbiome. The analysis of the proteomic data obtained by shotgun mass spectrometry have shown that several metabolic pathways are affected in response to nanoparticles, n-ZnO or n-TiO2, or zinc salt: the methyglyoxal and thiol metabolisms, the oxidative stress and the stringent responses. Nanoparticles being embedded in the agar medium, these impacts are the consequence of a physiological adaptation rather than a physical cell injury. Overall, these results show that nanoparticles, by altering bacterial physiology and especially the ability to resist to a stress, may have profound influences on a "good bacteria", Bacillus subtilis, in its natural biotope and moreover, on the global equilibrium of this biotope.https://doi.org/10.1371/journal.pone.0240510 |
| spellingShingle | Elise Eymard-Vernain Sylvie Luche Thierry Rabilloud Cécile Lelong ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. PLoS ONE |
| title | ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. |
| title_full | ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. |
| title_fullStr | ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. |
| title_full_unstemmed | ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. |
| title_short | ZnO and TiO2 nanoparticles alter the ability of Bacillus subtilis to fight against a stress. |
| title_sort | zno and tio2 nanoparticles alter the ability of bacillus subtilis to fight against a stress |
| url | https://doi.org/10.1371/journal.pone.0240510 |
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