Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells
ATP has broad functions as an autocrine/paracrine molecule. The mode of ATP release and its intracellular source, however, are little understood. Here we show that bradykinin via B2-receptor stimulation induces the extracellular release of ATP via the inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-sign...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319342161 |
| _version_ | 1811230898545229824 |
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| author | Yumei Zhao Keisuke Migita Chiemi Sato Sadaharu Usune Takahiro Iwamoto Takeshi Katsuragi |
| author_facet | Yumei Zhao Keisuke Migita Chiemi Sato Sadaharu Usune Takahiro Iwamoto Takeshi Katsuragi |
| author_sort | Yumei Zhao |
| collection | DOAJ |
| description | ATP has broad functions as an autocrine/paracrine molecule. The mode of ATP release and its intracellular source, however, are little understood. Here we show that bradykinin via B2-receptor stimulation induces the extracellular release of ATP via the inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-signaling pathway in cultured taenia coli smooth muscle cells. It was found that bradykinin also increased the production of Ins(1,4,5)P3 and 2-APB-inhibitable [Ca2+]i. The evoked release of ATP was suppressed by the Ca2+-channel blockers, nifedipine, and verapamil. Moreover, the extracellular release of ATP was elicited by photoliberation of Ins(1,4,5)P3. Bradykinin caused a quick and transient accumulation of intracellular ATP from cells treated with 1% perchloric acid solution (PCA), but not with the cell lysis buffer. Peak accumulation was prevented by 2-APB and thapsigargin, but not by nifedipine or verapamil, inhibitors of extracellular release of ATP. These findings suggest that bradykinin elicits the extracellular release of ATP that is mediated by the Ins(1,4,5)P3-induced Ca2+ signaling and, finally, leads to a Ca2+-dependent export of ATP from the cells. Furthermore, the bradykinin-induced transient accumulation of ATP in the cells treated with PCA may imply a possible release of ATP from the endoplasmic reticulum. Keywords:: bradykinin B2 receptor, extracellular ATP release, inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-mediated Ca2+ signaling, endoplasmic reticulum, cultured smooth muscle cell |
| first_indexed | 2024-04-12T10:35:49Z |
| format | Article |
| id | doaj.art-5c37756d76474faeadb3e0f6a445bfd2 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T10:35:49Z |
| publishDate | 2007-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-5c37756d76474faeadb3e0f6a445bfd22022-12-22T03:36:43ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-0110515765Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle CellsYumei Zhao0Keisuke Migita1Chiemi Sato2Sadaharu Usune3Takahiro Iwamoto4Takeshi Katsuragi5Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan; Department of Pedodontics, School of Dentistry of Shanghai Tongji University, Shanghai 200071, ChinaRehabilitation Medicine, Institute Brain Science, Hirosaki University School of Medicine, Hirosaki 036-8562 JapanDepartment of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, JapanResearch Laboratory of Biodynamics, School of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan; Medical Research Center, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan; Corresponding author. katsurag@fukuoka-u.ac.jpATP has broad functions as an autocrine/paracrine molecule. The mode of ATP release and its intracellular source, however, are little understood. Here we show that bradykinin via B2-receptor stimulation induces the extracellular release of ATP via the inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-signaling pathway in cultured taenia coli smooth muscle cells. It was found that bradykinin also increased the production of Ins(1,4,5)P3 and 2-APB-inhibitable [Ca2+]i. The evoked release of ATP was suppressed by the Ca2+-channel blockers, nifedipine, and verapamil. Moreover, the extracellular release of ATP was elicited by photoliberation of Ins(1,4,5)P3. Bradykinin caused a quick and transient accumulation of intracellular ATP from cells treated with 1% perchloric acid solution (PCA), but not with the cell lysis buffer. Peak accumulation was prevented by 2-APB and thapsigargin, but not by nifedipine or verapamil, inhibitors of extracellular release of ATP. These findings suggest that bradykinin elicits the extracellular release of ATP that is mediated by the Ins(1,4,5)P3-induced Ca2+ signaling and, finally, leads to a Ca2+-dependent export of ATP from the cells. Furthermore, the bradykinin-induced transient accumulation of ATP in the cells treated with PCA may imply a possible release of ATP from the endoplasmic reticulum. Keywords:: bradykinin B2 receptor, extracellular ATP release, inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-mediated Ca2+ signaling, endoplasmic reticulum, cultured smooth muscle cellhttp://www.sciencedirect.com/science/article/pii/S1347861319342161 |
| spellingShingle | Yumei Zhao Keisuke Migita Chiemi Sato Sadaharu Usune Takahiro Iwamoto Takeshi Katsuragi Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells Journal of Pharmacological Sciences |
| title | Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells |
| title_full | Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells |
| title_fullStr | Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells |
| title_full_unstemmed | Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells |
| title_short | Endoplasmic Reticulum Is a Key Organella in Bradykinin-Triggered ATP Release From Cultured Smooth Muscle Cells |
| title_sort | endoplasmic reticulum is a key organella in bradykinin triggered atp release from cultured smooth muscle cells |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319342161 |
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