In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages
Statins are successful drugs used to treat hypercholesterolemia, a primary cause of atherosclerosis. In this work, we investigated how hypercholesterolemia and pravastatin treatment impact macrophage and mitochondria functions, the key cell involved in atherogenesis. By comparing bone marrow-derived...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.839428/full |
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| author | Leandro Henrique de Paula Assis Gabriel de Gabriel Dorighello Thiago Rentz Jane Cristina de Souza Aníbal Eugênio Vercesi Helena Coutinho Franco de Oliveira |
| author_facet | Leandro Henrique de Paula Assis Gabriel de Gabriel Dorighello Thiago Rentz Jane Cristina de Souza Aníbal Eugênio Vercesi Helena Coutinho Franco de Oliveira |
| author_sort | Leandro Henrique de Paula Assis |
| collection | DOAJ |
| description | Statins are successful drugs used to treat hypercholesterolemia, a primary cause of atherosclerosis. In this work, we investigated how hypercholesterolemia and pravastatin treatment impact macrophage and mitochondria functions, the key cell involved in atherogenesis. By comparing bone marrow-derived macrophages (BMDM) of wild-type (WT) and LDL receptor knockout (LDLr−/−) mice, we observed hypercholesterolemia increased the number of contact sites at mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs), enhanced mitochondrial hydrogen peroxide release, altered the gene expression of inflammatory markers, and increased oxidized LDL (ox-LDL) uptake and phagocytic activity. Three months of in vivo pravastatin treatment of LDLr−/− mice reversed the number of contact sites at the MAM, ox-LDL uptake, and phagocytosis in LDLr−/− BMDM. Additionally, pravastatin increased BMDM mitochondrial network branching. In peritoneal macrophages (PMs), hypercholesterolemia did not change MAM stability, but stimulated hydrogen peroxide production and modulated gene expression of pro- and anti-inflammatory markers. It also increased mitochondrial branching degree and had no effects on ox-LDL uptake and phagocytosis in PM. Pravastatin treatment increased superoxide anion production and changed inflammation-related gene expression in LDLr−/− PM. In addition, pravastatin increased markedly the expression of the mitochondrial dynamics-related genes Mfn2 and Fis1 in both macrophages. In summary, our results show that hypercholesterolemia and pravastatin treatment affect macrophage mitochondria network structure as well as their interaction with the endoplasmic reticulum (ER). These effects impact on macrophage conversion rates to foam cell and macrophage phagocytic capacity. These findings associate MAM stability changes with known mechanisms involved in atherosclerosis progression and resolution. |
| first_indexed | 2024-12-22T16:31:41Z |
| format | Article |
| id | doaj.art-5c3995e8334346f194e9593f953e571b |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-22T16:31:41Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-5c3995e8334346f194e9593f953e571b2022-12-21T18:20:03ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-03-01910.3389/fmolb.2022.839428839428In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in MacrophagesLeandro Henrique de Paula Assis0Gabriel de Gabriel Dorighello1Thiago Rentz2Jane Cristina de Souza3Aníbal Eugênio Vercesi4Helena Coutinho Franco de Oliveira5Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, BrazilStatins are successful drugs used to treat hypercholesterolemia, a primary cause of atherosclerosis. In this work, we investigated how hypercholesterolemia and pravastatin treatment impact macrophage and mitochondria functions, the key cell involved in atherogenesis. By comparing bone marrow-derived macrophages (BMDM) of wild-type (WT) and LDL receptor knockout (LDLr−/−) mice, we observed hypercholesterolemia increased the number of contact sites at mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs), enhanced mitochondrial hydrogen peroxide release, altered the gene expression of inflammatory markers, and increased oxidized LDL (ox-LDL) uptake and phagocytic activity. Three months of in vivo pravastatin treatment of LDLr−/− mice reversed the number of contact sites at the MAM, ox-LDL uptake, and phagocytosis in LDLr−/− BMDM. Additionally, pravastatin increased BMDM mitochondrial network branching. In peritoneal macrophages (PMs), hypercholesterolemia did not change MAM stability, but stimulated hydrogen peroxide production and modulated gene expression of pro- and anti-inflammatory markers. It also increased mitochondrial branching degree and had no effects on ox-LDL uptake and phagocytosis in PM. Pravastatin treatment increased superoxide anion production and changed inflammation-related gene expression in LDLr−/− PM. In addition, pravastatin increased markedly the expression of the mitochondrial dynamics-related genes Mfn2 and Fis1 in both macrophages. In summary, our results show that hypercholesterolemia and pravastatin treatment affect macrophage mitochondria network structure as well as their interaction with the endoplasmic reticulum (ER). These effects impact on macrophage conversion rates to foam cell and macrophage phagocytic capacity. These findings associate MAM stability changes with known mechanisms involved in atherosclerosis progression and resolution.https://www.frontiersin.org/articles/10.3389/fmolb.2022.839428/fullhypercholesterolemiastatinmacrophagemitochondria-associated membranefoam cellphagocytosis |
| spellingShingle | Leandro Henrique de Paula Assis Gabriel de Gabriel Dorighello Thiago Rentz Jane Cristina de Souza Aníbal Eugênio Vercesi Helena Coutinho Franco de Oliveira In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages Frontiers in Molecular Biosciences hypercholesterolemia statin macrophage mitochondria-associated membrane foam cell phagocytosis |
| title | In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages |
| title_full | In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages |
| title_fullStr | In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages |
| title_full_unstemmed | In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages |
| title_short | In Vivo Pravastatin Treatment Reverses Hypercholesterolemia Induced Mitochondria-Associated Membranes Contact Sites, Foam Cell Formation, and Phagocytosis in Macrophages |
| title_sort | in vivo pravastatin treatment reverses hypercholesterolemia induced mitochondria associated membranes contact sites foam cell formation and phagocytosis in macrophages |
| topic | hypercholesterolemia statin macrophage mitochondria-associated membrane foam cell phagocytosis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.839428/full |
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