Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration
<i>Mycobacterium abscessus</i> (Mabs) is a dangerous non-tubercular mycobacterium responsible for severe pulmonary infections in immunologically vulnerable patients, due to its wide resistance to many different antibiotics which make its therapeutic management extremely difficult. Drug n...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/6/924 |
| _version_ | 1797595807553159168 |
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| author | Jacopo Forte Patrizia Nadia Hanieh Noemi Poerio Tommaso Olimpieri Maria Grazia Ammendolia Maurizio Fraziano Maria Gioia Fabiano Carlotta Marianecci Maria Carafa Federico Bordi Simona Sennato Federica Rinaldi |
| author_facet | Jacopo Forte Patrizia Nadia Hanieh Noemi Poerio Tommaso Olimpieri Maria Grazia Ammendolia Maurizio Fraziano Maria Gioia Fabiano Carlotta Marianecci Maria Carafa Federico Bordi Simona Sennato Federica Rinaldi |
| author_sort | Jacopo Forte |
| collection | DOAJ |
| description | <i>Mycobacterium abscessus</i> (Mabs) is a dangerous non-tubercular mycobacterium responsible for severe pulmonary infections in immunologically vulnerable patients, due to its wide resistance to many different antibiotics which make its therapeutic management extremely difficult. Drug nanocarriers as liposomes may represent a promising delivery strategy against pulmonary Mabs infection, due to the possibility to be aerosolically administrated and to tune their properties in order to increase nebulization resistance and retainment of encapsulated drug. In fact, liposome surface can be modified by decoration with mucoadhesive polymers to enhance its stability, mucus penetration and prolong its residence time in the lung. The aim of this work is to employ Chitosan or ε-poly-L-lysine decoration for improving the properties of a novel liposomes composed by hydrogenated phosphatidyl-choline from soybean (HSPC) and anionic 1,2-Dipalmitoyl-sn-glycero-3-phosphorylglycerol sodium salt (DPPG) able to entrap Rifampicin. A deep physicochemical characterization of polymer-decorated liposomes shows that both polymers improve mucoadhesion without affecting liposome features and Rifampicin entrapment efficiency. Therapeutic activity on Mabs-infected macrophages demonstrates an effective antibacterial effect of ε-poly-L-lysine liposomes with respect to chitosan-decorated ones. Altogether, these results suggest a possible use of ε-PLL liposomes to improve antibiotic delivery in the lung. |
| first_indexed | 2024-03-11T02:41:44Z |
| format | Article |
| id | doaj.art-5c3affe132c64d62acacaefefa3275ca |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T02:41:44Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-5c3affe132c64d62acacaefefa3275ca2023-11-18T09:30:44ZengMDPI AGBiomolecules2218-273X2023-05-0113692410.3390/biom13060924Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine DecorationJacopo Forte0Patrizia Nadia Hanieh1Noemi Poerio2Tommaso Olimpieri3Maria Grazia Ammendolia4Maurizio Fraziano5Maria Gioia Fabiano6Carlotta Marianecci7Maria Carafa8Federico Bordi9Simona Sennato10Federica Rinaldi11Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Biologia Università di Roma “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, ItalyDipartimento di Biologia Università di Roma “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, ItalyCentro Nazionale Tecnologie Innovative in Sanità Pubblica, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, ItalyDipartimento di Biologia Università di Roma “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, ItalyIstituto dei Sistemi Complessi (ISC)-CNR, sede “Sapienza” and Dipartimento di Fisica, Sapienza Università di Roma, 00185 Rome, ItalyIstituto dei Sistemi Complessi (ISC)-CNR, sede “Sapienza” and Dipartimento di Fisica, Sapienza Università di Roma, 00185 Rome, ItalyDipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy<i>Mycobacterium abscessus</i> (Mabs) is a dangerous non-tubercular mycobacterium responsible for severe pulmonary infections in immunologically vulnerable patients, due to its wide resistance to many different antibiotics which make its therapeutic management extremely difficult. Drug nanocarriers as liposomes may represent a promising delivery strategy against pulmonary Mabs infection, due to the possibility to be aerosolically administrated and to tune their properties in order to increase nebulization resistance and retainment of encapsulated drug. In fact, liposome surface can be modified by decoration with mucoadhesive polymers to enhance its stability, mucus penetration and prolong its residence time in the lung. The aim of this work is to employ Chitosan or ε-poly-L-lysine decoration for improving the properties of a novel liposomes composed by hydrogenated phosphatidyl-choline from soybean (HSPC) and anionic 1,2-Dipalmitoyl-sn-glycero-3-phosphorylglycerol sodium salt (DPPG) able to entrap Rifampicin. A deep physicochemical characterization of polymer-decorated liposomes shows that both polymers improve mucoadhesion without affecting liposome features and Rifampicin entrapment efficiency. Therapeutic activity on Mabs-infected macrophages demonstrates an effective antibacterial effect of ε-poly-L-lysine liposomes with respect to chitosan-decorated ones. Altogether, these results suggest a possible use of ε-PLL liposomes to improve antibiotic delivery in the lung.https://www.mdpi.com/2218-273X/13/6/924liposomesRifampicinpolymer decorationChitosanε-poly-L-lysine<i>Mycobacterium abscessus</i> |
| spellingShingle | Jacopo Forte Patrizia Nadia Hanieh Noemi Poerio Tommaso Olimpieri Maria Grazia Ammendolia Maurizio Fraziano Maria Gioia Fabiano Carlotta Marianecci Maria Carafa Federico Bordi Simona Sennato Federica Rinaldi Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration Biomolecules liposomes Rifampicin polymer decoration Chitosan ε-poly-L-lysine <i>Mycobacterium abscessus</i> |
| title | Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration |
| title_full | Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration |
| title_fullStr | Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration |
| title_full_unstemmed | Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration |
| title_short | Mucoadhesive Rifampicin-Liposomes for the Treatment of Pulmonary Infection by <i>Mycobacterium abscessus</i>: Chitosan or ε-Poly-L-Lysine Decoration |
| title_sort | mucoadhesive rifampicin liposomes for the treatment of pulmonary infection by i mycobacterium abscessus i chitosan or ε poly l lysine decoration |
| topic | liposomes Rifampicin polymer decoration Chitosan ε-poly-L-lysine <i>Mycobacterium abscessus</i> |
| url | https://www.mdpi.com/2218-273X/13/6/924 |
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