Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation
Objective: Heme oxygenase (HO) has been shown to have important antioxidant and anti-inflammatory properties, resulting in a vascular antitherogenic effect. This study was undertaken to evaluate the role of HO-2 in atherosclerosis. Method and results: The expression levels of HO-2 were evaluated in...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Pharmacology and Drug Discovery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259025712200061X |
| _version_ | 1827917713852334080 |
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| author | Zhenzhen Wang Xiaoqiang Zhan Shuai Yang Yang Chen Yingchao Bi Xuemei Xian Quangang Chen Xufeng Han Zhangping Yang Renjin Chen |
| author_facet | Zhenzhen Wang Xiaoqiang Zhan Shuai Yang Yang Chen Yingchao Bi Xuemei Xian Quangang Chen Xufeng Han Zhangping Yang Renjin Chen |
| author_sort | Zhenzhen Wang |
| collection | DOAJ |
| description | Objective: Heme oxygenase (HO) has been shown to have important antioxidant and anti-inflammatory properties, resulting in a vascular antitherogenic effect. This study was undertaken to evaluate the role of HO-2 in atherosclerosis. Method and results: The expression levels of HO-2 were evaluated in M1 and M2 bone marrow macrophage induced by LPS and IL4. The expression of HO-2 was significantly higher in M2 macrophage than in M1 macrophage. Western diet (WD) caused a significant increase in HO-2 expression in ApoE−/− mice. The adeno-associated viral (AAV) vectors expressing HO-2 was constructed, and the mice were received saline (ApoE−/−), AAV (ApoE−/−), AAV–HO–2 (ApoE−/−) on WD at 12 weeks and their plasma lipids, inflammatory cytokines, atherosclerosis were evaluated for 16 weeks. The results showed AAV–HO–2 was robust, with a significant decrease in the en face aortas, lipids levels, inflammatory cytokines and M1 macrophage content in AAV–HO–2 ApoE−/− compared to control AAV-ApoE−/−. Conclusion: HO-2 expression in macrophages plays an important role of the antiatherogenic effect, decreasing the inflammatory component of atherosclerotic lesions. These results suggest that HO-2 may be a novel therapeutic target for cardiovascular diseases. |
| first_indexed | 2024-03-13T03:32:22Z |
| format | Article |
| id | doaj.art-5c3b92eaef8346db95c07fcffaf7f660 |
| institution | Directory Open Access Journal |
| issn | 2590-2571 |
| language | English |
| last_indexed | 2024-03-13T03:32:22Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Pharmacology and Drug Discovery |
| spelling | doaj.art-5c3b92eaef8346db95c07fcffaf7f6602023-06-24T05:18:59ZengElsevierCurrent Research in Pharmacology and Drug Discovery2590-25712023-01-014100141Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammationZhenzhen Wang0Xiaoqiang Zhan1Shuai Yang2Yang Chen3Yingchao Bi4Xuemei Xian5Quangang Chen6Xufeng Han7Zhangping Yang8Renjin Chen9Cancer Institute, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaCollege of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaJiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, Yangzhou University, Yangzhou, Jiangsu, China; Corresponding author. Jiangsu Key Laboratory of Animal genetic Breeding and Molecular Design, Yangzhou University, Yangzhou, Jiangsu, China.College of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, China; Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, Yangzhou University, Yangzhou, Jiangsu, China; Corresponding author. College of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, China.Objective: Heme oxygenase (HO) has been shown to have important antioxidant and anti-inflammatory properties, resulting in a vascular antitherogenic effect. This study was undertaken to evaluate the role of HO-2 in atherosclerosis. Method and results: The expression levels of HO-2 were evaluated in M1 and M2 bone marrow macrophage induced by LPS and IL4. The expression of HO-2 was significantly higher in M2 macrophage than in M1 macrophage. Western diet (WD) caused a significant increase in HO-2 expression in ApoE−/− mice. The adeno-associated viral (AAV) vectors expressing HO-2 was constructed, and the mice were received saline (ApoE−/−), AAV (ApoE−/−), AAV–HO–2 (ApoE−/−) on WD at 12 weeks and their plasma lipids, inflammatory cytokines, atherosclerosis were evaluated for 16 weeks. The results showed AAV–HO–2 was robust, with a significant decrease in the en face aortas, lipids levels, inflammatory cytokines and M1 macrophage content in AAV–HO–2 ApoE−/− compared to control AAV-ApoE−/−. Conclusion: HO-2 expression in macrophages plays an important role of the antiatherogenic effect, decreasing the inflammatory component of atherosclerotic lesions. These results suggest that HO-2 may be a novel therapeutic target for cardiovascular diseases.http://www.sciencedirect.com/science/article/pii/S259025712200061XHeme oxygenase-2AtherosclerosisMacrophagesInflammation |
| spellingShingle | Zhenzhen Wang Xiaoqiang Zhan Shuai Yang Yang Chen Yingchao Bi Xuemei Xian Quangang Chen Xufeng Han Zhangping Yang Renjin Chen Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation Current Research in Pharmacology and Drug Discovery Heme oxygenase-2 Atherosclerosis Macrophages Inflammation |
| title | Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation |
| title_full | Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation |
| title_fullStr | Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation |
| title_full_unstemmed | Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation |
| title_short | Heme-heme oxygenase-2 reduces the atherosclerosis by preventing inflammation |
| title_sort | heme heme oxygenase 2 reduces the atherosclerosis by preventing inflammation |
| topic | Heme oxygenase-2 Atherosclerosis Macrophages Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S259025712200061X |
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