Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender
Prebeta-1 HDL is a molecular species of plasma HDL of approximately 67 kDa mass that contains apolipoprotein A-I, phospholipids, and unesterified cholesterol. It participates in a cyclic process involved in the retrieval of cholesterol from peripheral tissues. In this cycle, unesterified cholesterol...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1998-03-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520333046 |
| _version_ | 1823935295006965760 |
|---|---|
| author | P.M. O'Connor B.R. Zysow S.A. Schoenhaus B.Y. Ishida S.T. Kunitake J.M. Naya-Vigne P.N. Duchateau R.F. Redberg S.J. Spencer S. Mark M. Mazur D.C. Heilbron R.B. Jaffe M.J. Malloy J.P. Kane |
| author_facet | P.M. O'Connor B.R. Zysow S.A. Schoenhaus B.Y. Ishida S.T. Kunitake J.M. Naya-Vigne P.N. Duchateau R.F. Redberg S.J. Spencer S. Mark M. Mazur D.C. Heilbron R.B. Jaffe M.J. Malloy J.P. Kane |
| author_sort | P.M. O'Connor |
| collection | DOAJ |
| description | Prebeta-1 HDL is a molecular species of plasma HDL of approximately 67 kDa mass that contains apolipoprotein A-I, phospholipids, and unesterified cholesterol. It participates in a cyclic process involved in the retrieval of cholesterol from peripheral tissues. In this cycle, unesterified cholesterol from cells is incorporated into prebeta-1 HDL, providing a substrate for esterification of cholesterol by lecithin:cholesterol acyltransferase. Prebeta-1 HDL then becomes incorporated into larger HDL species of alpha mobility as esterification proceeds and is regenerated during the transfer of cholesteryl esters from alpha HDL particles to acceptor lipoproteins. Thus the steady state level of prebeta-1 HDL in plasma reflects the relative efficiencies of the major metabolic processes involved in its generation and removal. We have used an isotope dilution technique to measure prebeta-1 HDL levels in the plasmas of 136 normolipidemic individuals (46 M, 90 F). The mean absolute concentration of prebeta-1 HDL as apolipoprotein A-I was 68 ± 40 μg/ml for women, and 84 ± 49 m/ml for men. Prebeta-1 HDL represented 5.5 ± 3.3% of total apolipoprotein A-I in women, and 7.2 ± 4.0% in men. The distributions of both absolute and percent prebeta-1 HDL are highly asymmetric, with skew toward higher values. However, the skew appears not to be attributable to either plasma cholesterol or triglyceride levels which are also skewed in population samples. The percent prebeta-1 HDL was negatively correlated with HDL cholesterol levels (P < 0.0001), whereas absolute levels of prebeta-1 HDL were positively correlated with apolipoprotein A-I and negatively correlated with HDL cholesterol (P, for both, < 0.0001). Multiple linear regression analysis revealed effects of age and gender, but no association with lipoprotein fractions other than HDL. Lower levels of prebeta-1 HDL were associated with female gender in all models.—O'Connor, P. M., B. R. Zysow, S. A. Schoenhaus, B. Y. Ishida, S. T. Kunitake, J. M. Naya-Vigne, P. N. Duchateau, R. F. Redberg, S. J. Spencer, S. Mark, M. Mazur, D. C. Heilbron, R. B. Jaffe, M. J. Malloy, and J. P. Kane. Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma, age, and gender. |
| first_indexed | 2024-12-16T22:35:23Z |
| format | Article |
| id | doaj.art-5c3d497dc4bd4ed995e660626e0e0cce |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T22:35:23Z |
| publishDate | 1998-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5c3d497dc4bd4ed995e660626e0e0cce2022-12-21T22:13:38ZengElsevierJournal of Lipid Research0022-22751998-03-01393670678Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and genderP.M. O'Connor0B.R. Zysow1S.A. Schoenhaus2B.Y. Ishida3S.T. Kunitake4J.M. Naya-Vigne5P.N. Duchateau6R.F. Redberg7S.J. Spencer8S. Mark9M. Mazur10D.C. Heilbron11R.B. Jaffe12M.J. Malloy13J.P. Kane14Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Genentech, Inc., South San Francisco, CACardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Biometric Imaging, Mountain View, CACardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Reproductive Endocrinology Center, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California-San Francisco, San Francisco, CAReproductive Endocrinology Center, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California-San Francisco, San Francisco, CACardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Reproductive Endocrinology Center, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California-San Francisco, San Francisco, CACardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143To whom correspondence should be addressed.; Cardiovascular Research Institute, University of California-San Francisco, San Francisco, CA 94143Prebeta-1 HDL is a molecular species of plasma HDL of approximately 67 kDa mass that contains apolipoprotein A-I, phospholipids, and unesterified cholesterol. It participates in a cyclic process involved in the retrieval of cholesterol from peripheral tissues. In this cycle, unesterified cholesterol from cells is incorporated into prebeta-1 HDL, providing a substrate for esterification of cholesterol by lecithin:cholesterol acyltransferase. Prebeta-1 HDL then becomes incorporated into larger HDL species of alpha mobility as esterification proceeds and is regenerated during the transfer of cholesteryl esters from alpha HDL particles to acceptor lipoproteins. Thus the steady state level of prebeta-1 HDL in plasma reflects the relative efficiencies of the major metabolic processes involved in its generation and removal. We have used an isotope dilution technique to measure prebeta-1 HDL levels in the plasmas of 136 normolipidemic individuals (46 M, 90 F). The mean absolute concentration of prebeta-1 HDL as apolipoprotein A-I was 68 ± 40 μg/ml for women, and 84 ± 49 m/ml for men. Prebeta-1 HDL represented 5.5 ± 3.3% of total apolipoprotein A-I in women, and 7.2 ± 4.0% in men. The distributions of both absolute and percent prebeta-1 HDL are highly asymmetric, with skew toward higher values. However, the skew appears not to be attributable to either plasma cholesterol or triglyceride levels which are also skewed in population samples. The percent prebeta-1 HDL was negatively correlated with HDL cholesterol levels (P < 0.0001), whereas absolute levels of prebeta-1 HDL were positively correlated with apolipoprotein A-I and negatively correlated with HDL cholesterol (P, for both, < 0.0001). Multiple linear regression analysis revealed effects of age and gender, but no association with lipoprotein fractions other than HDL. Lower levels of prebeta-1 HDL were associated with female gender in all models.—O'Connor, P. M., B. R. Zysow, S. A. Schoenhaus, B. Y. Ishida, S. T. Kunitake, J. M. Naya-Vigne, P. N. Duchateau, R. F. Redberg, S. J. Spencer, S. Mark, M. Mazur, D. C. Heilbron, R. B. Jaffe, M. J. Malloy, and J. P. Kane. Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma, age, and gender.http://www.sciencedirect.com/science/article/pii/S0022227520333046apolipoprotein A-Ilecithin:cholesterol acyltransferasecholesteryl ester transfer proteincholesterol transport |
| spellingShingle | P.M. O'Connor B.R. Zysow S.A. Schoenhaus B.Y. Ishida S.T. Kunitake J.M. Naya-Vigne P.N. Duchateau R.F. Redberg S.J. Spencer S. Mark M. Mazur D.C. Heilbron R.B. Jaffe M.J. Malloy J.P. Kane Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender Journal of Lipid Research apolipoprotein A-I lecithin:cholesterol acyltransferase cholesteryl ester transfer protein cholesterol transport |
| title | Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender |
| title_full | Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender |
| title_fullStr | Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender |
| title_full_unstemmed | Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender |
| title_short | Prebeta-1 HDL in plasma of normolipidemic individuals: influences of plasma lipoproteins, age, and gender |
| title_sort | prebeta 1 hdl in plasma of normolipidemic individuals influences of plasma lipoproteins age and gender |
| topic | apolipoprotein A-I lecithin:cholesterol acyltransferase cholesteryl ester transfer protein cholesterol transport |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520333046 |
| work_keys_str_mv | AT pmoconnor prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT brzysow prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT saschoenhaus prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT byishida prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT stkunitake prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT jmnayavigne prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT pnduchateau prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT rfredberg prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT sjspencer prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT smark prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT mmazur prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT dcheilbron prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT rbjaffe prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT mjmalloy prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender AT jpkane prebeta1hdlinplasmaofnormolipidemicindividualsinfluencesofplasmalipoproteinsageandgender |