Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice
The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Rice Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630820300792 |
| _version_ | 1818173099507449856 |
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| author | Li Ruili Wang Jiaoling Xu Lei Sun Meihao Yi Keke Zhao Hongyu |
| author_facet | Li Ruili Wang Jiaoling Xu Lei Sun Meihao Yi Keke Zhao Hongyu |
| author_sort | Li Ruili |
| collection | DOAJ |
| description | The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4 gene family in rice. Together with the PHT4 genes from higher plants, OsPHT4s can be classified into six distinct groups. OsPHT4;1–OsPHT4;4 are targeted to chloroplasts, and OsPHT4;6-1 and OsPHT4;6-2 are located to Golgi apparatus. OsPHT4 proteins can mediate inorganic phosphate (Pi) transport in yeast. In addition, dynamic transcriptional changes determined by qRT-PCR revealed different expression profiles of OsPHT4 genes in response to phosphate starvation, salicylic acid, abscisic acid and salt stress treatments. These results suggested that OsPHT4 proteins are involved in Pi distribution between the cytoplasm and chloroplast or Golgi apparatus and also involved in stress responses. |
| first_indexed | 2024-12-11T19:23:07Z |
| format | Article |
| id | doaj.art-5c3df9b200494f2897daaaccc277e3b5 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-11T19:23:07Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-5c3df9b200494f2897daaaccc277e3b52022-12-22T00:53:28ZengElsevierRice Science1672-63082020-11-01276493503Functional Analysis of Phosphate Transporter OsPHT4 Family Members in RiceLi Ruili0Wang Jiaoling1Xu Lei2Sun Meihao3Yi Keke4Zhao Hongyu5College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Science and Technology, Nanyang Normal University, Nanyang 473061, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author.The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4 gene family in rice. Together with the PHT4 genes from higher plants, OsPHT4s can be classified into six distinct groups. OsPHT4;1–OsPHT4;4 are targeted to chloroplasts, and OsPHT4;6-1 and OsPHT4;6-2 are located to Golgi apparatus. OsPHT4 proteins can mediate inorganic phosphate (Pi) transport in yeast. In addition, dynamic transcriptional changes determined by qRT-PCR revealed different expression profiles of OsPHT4 genes in response to phosphate starvation, salicylic acid, abscisic acid and salt stress treatments. These results suggested that OsPHT4 proteins are involved in Pi distribution between the cytoplasm and chloroplast or Golgi apparatus and also involved in stress responses.http://www.sciencedirect.com/science/article/pii/S1672630820300792ricephosphate transporterabiotic stresssubcellular localizationexpression profile |
| spellingShingle | Li Ruili Wang Jiaoling Xu Lei Sun Meihao Yi Keke Zhao Hongyu Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice Rice Science rice phosphate transporter abiotic stress subcellular localization expression profile |
| title | Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice |
| title_full | Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice |
| title_fullStr | Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice |
| title_full_unstemmed | Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice |
| title_short | Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice |
| title_sort | functional analysis of phosphate transporter ospht4 family members in rice |
| topic | rice phosphate transporter abiotic stress subcellular localization expression profile |
| url | http://www.sciencedirect.com/science/article/pii/S1672630820300792 |
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