Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment
Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom. Methods: Mango seed kernel methanolic extract was evaluated in vitro...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2017-09-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | https://ijt.arakmu.ac.ir/article-1-557-en.pdf |
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| author | Peculiar Nwanyibunwa Okoro* Sani Ibrahim Hajiya Mairo Inuwa Stanley Irobekhian Reuben Okoduwa |
| author_facet | Peculiar Nwanyibunwa Okoro* Sani Ibrahim Hajiya Mairo Inuwa Stanley Irobekhian Reuben Okoduwa |
| author_sort | Peculiar Nwanyibunwa Okoro* |
| collection | DOAJ |
| description | Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom.
Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 µmol/min/mg protein.
Results: The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Km of 0.31 mg mL-1 and a Vmax of 9.09 µmol min-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Km values of 0.56 and 1.11 mg mL-1 and Vmaxvalues of 6.67 and 4.17-µmol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Ki value of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor.
Conclusion: Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety. |
| first_indexed | 2024-04-12T15:42:00Z |
| format | Article |
| id | doaj.art-5c40372b3bc84a76a23d23900e93a539 |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-04-12T15:42:00Z |
| publishDate | 2017-09-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-5c40372b3bc84a76a23d23900e93a5392022-12-22T03:26:45ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592017-09-011152330Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro ExperimentPeculiar Nwanyibunwa Okoro* 0Sani Ibrahim 1Hajiya Mairo Inuwa2Stanley Irobekhian Reuben Okoduwa3Department of Basic Research, National Research Institute for Chemical Technology, Zaria, Nigeria. *Corresponding Author: E-Mail: peckyclett@sironigeria.comDepartment of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.Department of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom. Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 µmol/min/mg protein. Results: The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Km of 0.31 mg mL-1 and a Vmax of 9.09 µmol min-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Km values of 0.56 and 1.11 mg mL-1 and Vmaxvalues of 6.67 and 4.17-µmol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Ki value of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor. Conclusion: Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety.https://ijt.arakmu.ac.ir/article-1-557-en.pdfMangiferaMetalloproteasesSnake BitesViperidaeViper Venoms |
| spellingShingle | Peculiar Nwanyibunwa Okoro* Sani Ibrahim Hajiya Mairo Inuwa Stanley Irobekhian Reuben Okoduwa Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment Iranian Journal of Toxicology Mangifera Metalloproteases Snake Bites Viperidae Viper Venoms |
| title | Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment |
| title_full | Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment |
| title_fullStr | Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment |
| title_full_unstemmed | Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment |
| title_short | Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment |
| title_sort | evaluation of mango seed kernel methanolic extract on metalloproteases in carpet viper echisocellatus venom an in vitro experiment |
| topic | Mangifera Metalloproteases Snake Bites Viperidae Viper Venoms |
| url | https://ijt.arakmu.ac.ir/article-1-557-en.pdf |
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