Syntaxin 7 modulates seizure activity in epilepsy
The exact pathogenesis of epilepsy, one of the most common and devastating diseases of the nervous system, is not fully understood. Syntaxin7 (STX7) is a member of the SNARE superfamily, which mediates membrane fusion events in all cells. However, the role STX7 plays in epilepsy remains unclear. The...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996123001328 |
| _version_ | 1797827772919316480 |
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| author | Junhong Wu Hui Zhang Liu Yang Yuanyuan Chen Jiyuan Li Min Yang Xiaogang Zhang Changlong He Xuefeng Wang Xin Xu |
| author_facet | Junhong Wu Hui Zhang Liu Yang Yuanyuan Chen Jiyuan Li Min Yang Xiaogang Zhang Changlong He Xuefeng Wang Xin Xu |
| author_sort | Junhong Wu |
| collection | DOAJ |
| description | The exact pathogenesis of epilepsy, one of the most common and devastating diseases of the nervous system, is not fully understood. Syntaxin7 (STX7) is a member of the SNARE superfamily, which mediates membrane fusion events in all cells. However, the role STX7 plays in epilepsy remains unclear. Therefore, this study investigates the role of STX7 in epilepsy. Our study found that the expression of STX7 was reduced in the epileptic brain and that overexpression of STX7 decreased the susceptibility to epileptic seizures and alleviated epileptic activity in a kainic acid–induced model and pentylenetetrazole-induced kindling model of epilepsy, whereas the downregulation of STX7 showed opposite effects. Whole-cell patch-clamp recordings showed that STX7 does not affect the intrinsic excitability of neurons, but rather the excitation/inhibition ratio mediated by affecting the release of presynaptic γ-aminobutyric acid neurotransmitters. Transmission electron microscopy results showed that STX7 did not affect the density of inhibitory synapses but could affect the density of inhibitory vesicles. Taken together, these results reveal a previously unknown function of STX7 in epilepsy and suggest that STX7 may serve as a novel target for epilepsy therapy. |
| first_indexed | 2024-04-09T12:53:44Z |
| format | Article |
| id | doaj.art-5c413e7d99d74a098462d8d91331af13 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-04-09T12:53:44Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-5c413e7d99d74a098462d8d91331af132023-05-14T04:28:10ZengElsevierNeurobiology of Disease1095-953X2023-06-01181106118Syntaxin 7 modulates seizure activity in epilepsyJunhong Wu0Hui Zhang1Liu Yang2Yuanyuan Chen3Jiyuan Li4Min Yang5Xiaogang Zhang6Changlong He7Xuefeng Wang8Xin Xu9Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Neurology, The First Hospital of Shanxi Medical University, No.85 Jiefang South Road, Taiyuan, Shanxi Province, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China; Department of Neurology, Chongqing General Hospital, Chongqing Key Laboratory of Neurodegenerative Diseases, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing 401147, ChinaDepartment of Laboratory Medicine, People's Hospital of Jiulongpo District, Chongqing 400016, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Corresponding author at: Department of Laboratory Medicine, People's Hospital of Jiulongpo District, Chongqing 40016, China.Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China; Corresponding authors.Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China; Corresponding authors.The exact pathogenesis of epilepsy, one of the most common and devastating diseases of the nervous system, is not fully understood. Syntaxin7 (STX7) is a member of the SNARE superfamily, which mediates membrane fusion events in all cells. However, the role STX7 plays in epilepsy remains unclear. Therefore, this study investigates the role of STX7 in epilepsy. Our study found that the expression of STX7 was reduced in the epileptic brain and that overexpression of STX7 decreased the susceptibility to epileptic seizures and alleviated epileptic activity in a kainic acid–induced model and pentylenetetrazole-induced kindling model of epilepsy, whereas the downregulation of STX7 showed opposite effects. Whole-cell patch-clamp recordings showed that STX7 does not affect the intrinsic excitability of neurons, but rather the excitation/inhibition ratio mediated by affecting the release of presynaptic γ-aminobutyric acid neurotransmitters. Transmission electron microscopy results showed that STX7 did not affect the density of inhibitory synapses but could affect the density of inhibitory vesicles. Taken together, these results reveal a previously unknown function of STX7 in epilepsy and suggest that STX7 may serve as a novel target for epilepsy therapy.http://www.sciencedirect.com/science/article/pii/S0969996123001328Syntaxin 7EpilepsySynaptic transmissionSynaptic vesicleGABA |
| spellingShingle | Junhong Wu Hui Zhang Liu Yang Yuanyuan Chen Jiyuan Li Min Yang Xiaogang Zhang Changlong He Xuefeng Wang Xin Xu Syntaxin 7 modulates seizure activity in epilepsy Neurobiology of Disease Syntaxin 7 Epilepsy Synaptic transmission Synaptic vesicle GABA |
| title | Syntaxin 7 modulates seizure activity in epilepsy |
| title_full | Syntaxin 7 modulates seizure activity in epilepsy |
| title_fullStr | Syntaxin 7 modulates seizure activity in epilepsy |
| title_full_unstemmed | Syntaxin 7 modulates seizure activity in epilepsy |
| title_short | Syntaxin 7 modulates seizure activity in epilepsy |
| title_sort | syntaxin 7 modulates seizure activity in epilepsy |
| topic | Syntaxin 7 Epilepsy Synaptic transmission Synaptic vesicle GABA |
| url | http://www.sciencedirect.com/science/article/pii/S0969996123001328 |
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