First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel
IntroductionMicroorganisms colonize a wide range of natural and artificial environments. Even though most of them are unculturable in laboratory conditions, some ecosystems are ideal niches for bioprospecting extremophiles with unique properties. Up today, there are few reports concerning microbial...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.982422/full |
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| author | Sara Baldanta Raquel Arnal Amaya Blanco-Rivero Govinda Guevara Juana María Navarro Llorens |
| author_facet | Sara Baldanta Raquel Arnal Amaya Blanco-Rivero Govinda Guevara Juana María Navarro Llorens |
| author_sort | Sara Baldanta |
| collection | DOAJ |
| description | IntroductionMicroorganisms colonize a wide range of natural and artificial environments. Even though most of them are unculturable in laboratory conditions, some ecosystems are ideal niches for bioprospecting extremophiles with unique properties. Up today, there are few reports concerning microbial communities found on solar panels, a widespread, artificial, extreme habitat. Microorganisms found in this habitat belong to drought-, heat- and radiation-adapted genera, including fungi, bacteria, and cyanobacteria.MethodsHere we isolated and identified several cyanobacteria from a solar panel. Then, some strains isolated were characterizated for their resistance to desiccation, UV-C exposition, and their growth on a range of temperature, pH, NaCl concentration or diverse carbon and nitrogen sources. Finally, gene transfer to these isolates was evaluated using several SEVA plasmids with different replicons to assess their potential in biotechnological applications.Results and discussionThis study presents the first identification and characterization of cultivable extremophile cyanobacteria from a solar panel in Valencia, Spain. The isolates are members of the genera Chroococcidiopsis, Leptolyngbya, Myxacorys, and Oculatella all genera with species commonly isolated from deserts and arid regions. Four of the isolates were selected, all of them Chroococcidiopsis, and characterized. Our results showed that all Chroococcidiopsis isolates chosen were resistant up to a year of desiccation, viable after exposition to high doses of UV-C, and capable of being transformed. Our findings revealed that a solar panel is a useful ecological niche in searching for extremophilic cyanobacteria to further study the desiccation and UV-tolerance mechanisms. We conclude that these cyanobacteria can be modified and exploited as candidates for biotechnological purposes, including astrobiology applications. |
| first_indexed | 2024-04-10T09:40:46Z |
| format | Article |
| id | doaj.art-5c42ea6492044b938b0383eafe1e173e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T09:40:46Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5c42ea6492044b938b0383eafe1e173e2023-02-17T09:04:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.982422982422First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panelSara BaldantaRaquel ArnalAmaya Blanco-RiveroGovinda GuevaraJuana María Navarro LlorensIntroductionMicroorganisms colonize a wide range of natural and artificial environments. Even though most of them are unculturable in laboratory conditions, some ecosystems are ideal niches for bioprospecting extremophiles with unique properties. Up today, there are few reports concerning microbial communities found on solar panels, a widespread, artificial, extreme habitat. Microorganisms found in this habitat belong to drought-, heat- and radiation-adapted genera, including fungi, bacteria, and cyanobacteria.MethodsHere we isolated and identified several cyanobacteria from a solar panel. Then, some strains isolated were characterizated for their resistance to desiccation, UV-C exposition, and their growth on a range of temperature, pH, NaCl concentration or diverse carbon and nitrogen sources. Finally, gene transfer to these isolates was evaluated using several SEVA plasmids with different replicons to assess their potential in biotechnological applications.Results and discussionThis study presents the first identification and characterization of cultivable extremophile cyanobacteria from a solar panel in Valencia, Spain. The isolates are members of the genera Chroococcidiopsis, Leptolyngbya, Myxacorys, and Oculatella all genera with species commonly isolated from deserts and arid regions. Four of the isolates were selected, all of them Chroococcidiopsis, and characterized. Our results showed that all Chroococcidiopsis isolates chosen were resistant up to a year of desiccation, viable after exposition to high doses of UV-C, and capable of being transformed. Our findings revealed that a solar panel is a useful ecological niche in searching for extremophilic cyanobacteria to further study the desiccation and UV-tolerance mechanisms. We conclude that these cyanobacteria can be modified and exploited as candidates for biotechnological purposes, including astrobiology applications.https://www.frontiersin.org/articles/10.3389/fmicb.2023.982422/fullextremophile cyanobacteriasolar paneldesiccationUV-CChroococcidiopsisSEVA vectors |
| spellingShingle | Sara Baldanta Raquel Arnal Amaya Blanco-Rivero Govinda Guevara Juana María Navarro Llorens First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel Frontiers in Microbiology extremophile cyanobacteria solar panel desiccation UV-C Chroococcidiopsis SEVA vectors |
| title | First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel |
| title_full | First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel |
| title_fullStr | First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel |
| title_full_unstemmed | First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel |
| title_short | First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel |
| title_sort | first characterization of cultivable extremophile chroococcidiopsis isolates from a solar panel |
| topic | extremophile cyanobacteria solar panel desiccation UV-C Chroococcidiopsis SEVA vectors |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.982422/full |
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