| Summary: | The CYSTM (cysteine-rich transmembrane module) protein family comprises small molecular cysteine-rich tail-anchored membrane proteins found in many eukaryotes. The <i>Saccharomyces cerevisiae</i> strains carrying the CYSTM genes <i>YDRO34W-B</i> and <i>YBR056W-A</i> (<i>MNC1</i>) fused with <i>GFP</i> were used to test the expression of these genes under different stresses. The <i>YBR056W-A</i> (<i>MNC1</i>) and <i>YDR034W-B</i> genes are expressed under stress conditions caused by the toxic concentrations of heavy metal ions, such as manganese, cobalt, nickel, zinc, cuprum, and 2.4-dinitrophenol uncoupler. The expression level of <i>YDR034W-B</i> was higher than that of <i>YBR056W-A</i> under alkali and cadmium stresses. The Ydr034w-b-GFP and Ybr056w-a-GFP proteins differ in the cellular localization: Ydr034w-b-GFP was mainly observed in the plasma membrane and vacuolar membrane, while Ybr056w-a-GFP was observed in the cytoplasm, probably in intracellular membranes. The null-mutants in both genes demonstrated decreased cell concentration and lytic phenotype when cultivated in the presence of excess manganese. This allows for speculations about the involvement of Mnc1 and Ydr034w-b proteins in manganese stress overcoming.
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