3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects
In patients with diabetic wounds, wound healing is impaired due to the presence of persistent oxidative stress, an altered inflammatory response, and impaired angiogenesis and epithelization. Salvianolic acid B (SAB), which is derived from the Chinese medicinal plant Salvia miltiorrhiza, has been fo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332224000490 |
| _version_ | 1797324333251559424 |
|---|---|
| author | Qin Lihao Liu Tingting Zhang Jiawei Bai Yifei Tang Zheyu Li Jingyan Xue Tongqing Jia Zhongzhi |
| author_facet | Qin Lihao Liu Tingting Zhang Jiawei Bai Yifei Tang Zheyu Li Jingyan Xue Tongqing Jia Zhongzhi |
| author_sort | Qin Lihao |
| collection | DOAJ |
| description | In patients with diabetic wounds, wound healing is impaired due to the presence of persistent oxidative stress, an altered inflammatory response, and impaired angiogenesis and epithelization. Salvianolic acid B (SAB), which is derived from the Chinese medicinal plant Salvia miltiorrhiza, has been found to exhibit antioxidant, anti-inflammatory, and proangiogenic effects. Previous studies have used 3D bioprinting technology incorporating sodium alginate (SA) and gelatin (Gel) as basic biomaterials to successfully produce artificial skin. In the current study, 3D bioprinting technology was used to incorporate SAB into SA-Gel to form a novel SAB-SA-Gel composite porous scaffold. The morphological characteristics, physicochemical characteristics, biocompatibility, and SAB release profile of the SAB-SA-Gel scaffolds were evaluated in vitro. In addition, the antioxidant, anti-inflammatory, and proangiogenic abilities of the SAB-SA-Gel scaffolds were evaluated in cells and in a rat model. Analysis demonstrated that 1.0 wt% (the percentage of SAB in the total weight of the solution containing SA and Gel) SAB-SA-Gel scaffolds had strong antioxidant, anti-inflammatory, and proangiogenic properties both in cells and in the rat model. The 1.0% SAB-SA-Gel scaffold reduced the expression of tumor necrosis factor-α, interleukin-6, and interluekin-1β and increased the expression of transforming growth factor-β. In addition, this scaffold removed excessive reactive oxygen species by increasing the expression of superoxide dismutase, thereby protecting fibroblasts from injury. The scaffold increased the expression of vascular endothelial growth factor and platelet/endothelial cell adhesion molecule-1, accelerated granulation tissue regeneration and collagen deposition, and promoted wound healing. These findings suggest that this innovative scaffold may have promise as a simple and efficient approach to managing diabetic wound repair. |
| first_indexed | 2024-03-08T05:54:37Z |
| format | Article |
| id | doaj.art-5c45514ac66246219a7b9798bb9f95c7 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-08T05:54:37Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-5c45514ac66246219a7b9798bb9f95c72024-02-05T04:31:21ZengElsevierBiomedicine & Pharmacotherapy0753-33222024-02-011711161683D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effectsQin Lihao0Liu Tingting1Zhang Jiawei2Bai Yifei3Tang Zheyu4Li Jingyan5Xue Tongqing6Jia Zhongzhi7Department of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, ChinaGraduate College, Dalian Medical University, Dalian 116044, ChinaDepartment of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, ChinaDepartment of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, ChinaDepartment of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, ChinaDepartment of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, China; Corresponding authors.Department of Interventional Radiology, Huaian Hospital of Huai'an City (Huaian Cancer Hospital), Huai'an 223200, China; Corresponding authors.Department of Interventional and Vascular Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University, Changzhou 213003, China; Corresponding authors.In patients with diabetic wounds, wound healing is impaired due to the presence of persistent oxidative stress, an altered inflammatory response, and impaired angiogenesis and epithelization. Salvianolic acid B (SAB), which is derived from the Chinese medicinal plant Salvia miltiorrhiza, has been found to exhibit antioxidant, anti-inflammatory, and proangiogenic effects. Previous studies have used 3D bioprinting technology incorporating sodium alginate (SA) and gelatin (Gel) as basic biomaterials to successfully produce artificial skin. In the current study, 3D bioprinting technology was used to incorporate SAB into SA-Gel to form a novel SAB-SA-Gel composite porous scaffold. The morphological characteristics, physicochemical characteristics, biocompatibility, and SAB release profile of the SAB-SA-Gel scaffolds were evaluated in vitro. In addition, the antioxidant, anti-inflammatory, and proangiogenic abilities of the SAB-SA-Gel scaffolds were evaluated in cells and in a rat model. Analysis demonstrated that 1.0 wt% (the percentage of SAB in the total weight of the solution containing SA and Gel) SAB-SA-Gel scaffolds had strong antioxidant, anti-inflammatory, and proangiogenic properties both in cells and in the rat model. The 1.0% SAB-SA-Gel scaffold reduced the expression of tumor necrosis factor-α, interleukin-6, and interluekin-1β and increased the expression of transforming growth factor-β. In addition, this scaffold removed excessive reactive oxygen species by increasing the expression of superoxide dismutase, thereby protecting fibroblasts from injury. The scaffold increased the expression of vascular endothelial growth factor and platelet/endothelial cell adhesion molecule-1, accelerated granulation tissue regeneration and collagen deposition, and promoted wound healing. These findings suggest that this innovative scaffold may have promise as a simple and efficient approach to managing diabetic wound repair.http://www.sciencedirect.com/science/article/pii/S0753332224000490DiabetesWound3D bioprintingSkin scaffoldSalvianolic acid B |
| spellingShingle | Qin Lihao Liu Tingting Zhang Jiawei Bai Yifei Tang Zheyu Li Jingyan Xue Tongqing Jia Zhongzhi 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects Biomedicine & Pharmacotherapy Diabetes Wound 3D bioprinting Skin scaffold Salvianolic acid B |
| title | 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects |
| title_full | 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects |
| title_fullStr | 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects |
| title_full_unstemmed | 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects |
| title_short | 3D bioprinting of Salvianolic acid B-sodium alginate-gelatin skin scaffolds promotes diabetic wound repair via antioxidant, anti-inflammatory, and proangiogenic effects |
| title_sort | 3d bioprinting of salvianolic acid b sodium alginate gelatin skin scaffolds promotes diabetic wound repair via antioxidant anti inflammatory and proangiogenic effects |
| topic | Diabetes Wound 3D bioprinting Skin scaffold Salvianolic acid B |
| url | http://www.sciencedirect.com/science/article/pii/S0753332224000490 |
| work_keys_str_mv | AT qinlihao 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT liutingting 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT zhangjiawei 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT baiyifei 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT tangzheyu 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT lijingyan 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT xuetongqing 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects AT jiazhongzhi 3dbioprintingofsalvianolicacidbsodiumalginategelatinskinscaffoldspromotesdiabeticwoundrepairviaantioxidantantiinflammatoryandproangiogeniceffects |