Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars
Abstract In 2013, PED emerged for the first time in the United States (US). The porcine epidemic diarrhea virus (PEDV) spread quickly throughout North America. Infection with PEDV causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-InDel strains circula...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-018-0505-2 |
| _version_ | 1811342121383231488 |
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| author | Sarah Gallien Angélique Moro Gérald Lediguerher Virginie Catinot Frédéric Paboeuf Lionel Bigault Mustapha Berri Phillip C. Gauger Nathalie Pozzi Edith Authié Nicolas Rose Béatrice Grasland |
| author_facet | Sarah Gallien Angélique Moro Gérald Lediguerher Virginie Catinot Frédéric Paboeuf Lionel Bigault Mustapha Berri Phillip C. Gauger Nathalie Pozzi Edith Authié Nicolas Rose Béatrice Grasland |
| author_sort | Sarah Gallien |
| collection | DOAJ |
| description | Abstract In 2013, PED emerged for the first time in the United States (US). The porcine epidemic diarrhea virus (PEDV) spread quickly throughout North America. Infection with PEDV causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-InDel strains circulating in the US. PEDV is mainly transmitted by the fecal–oral route. Transmission via the venereal route has been suspected but not previously investigated. The aim of the study was to determine if PEDV could be detected in semen from infected specific pathogen-free (SPF) boars inoculated with a PEDV US non-InDel strain suggesting venereal transmission may occur. Two boars orally inoculated with PEDV showed clinical signs and virus shedding in feces. Transient presence of the PEDV genome was detected by RT-qPCR in the seminal (5.06 × 102 to 2.44 × 103 genomic copies/mL) and sperm-rich fraction of semen (5.64 × 102 to 3.40 × 104 genomic copies/mL) and a longer duration of viral shedding was observed in the sperm-rich fraction. The evidence of PEDV shedding in semen raises new questions in term of disease spread within the pig population with the use of potentially contaminated semen. |
| first_indexed | 2024-04-13T19:05:48Z |
| format | Article |
| id | doaj.art-5c48620719b14d119d71aa45b0f28afe |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-04-13T19:05:48Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-5c48620719b14d119d71aa45b0f28afe2022-12-22T02:33:59ZengBMCVeterinary Research1297-97162018-01-014911910.1186/s13567-018-0505-2Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boarsSarah Gallien0Angélique Moro1Gérald Lediguerher2Virginie Catinot3Frédéric Paboeuf4Lionel Bigault5Mustapha Berri6Phillip C. Gauger7Nathalie Pozzi8Edith Authié9Nicolas Rose10Béatrice Grasland11Anses, Laboratory of Ploufragan/PlouzanéAnses, Laboratory of Ploufragan/PlouzanéAnses, Laboratory of Ploufragan/PlouzanéLaboratoire National de Contrôle des Reproducteurs (LNCR)Anses, Laboratory of Ploufragan/PlouzanéAnses, Laboratory of Ploufragan/PlouzanéInstitut National de Recherche Agronomique (INRA), UMR 1282, Université François RabelaisVeterinary Diagnostic & Production Animal Medicine, Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State UniversityLaboratoire National de Contrôle des Reproducteurs (LNCR)Laboratoire National de Contrôle des Reproducteurs (LNCR)Anses, Laboratory of Ploufragan/PlouzanéAnses, Laboratory of Ploufragan/PlouzanéAbstract In 2013, PED emerged for the first time in the United States (US). The porcine epidemic diarrhea virus (PEDV) spread quickly throughout North America. Infection with PEDV causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-InDel strains circulating in the US. PEDV is mainly transmitted by the fecal–oral route. Transmission via the venereal route has been suspected but not previously investigated. The aim of the study was to determine if PEDV could be detected in semen from infected specific pathogen-free (SPF) boars inoculated with a PEDV US non-InDel strain suggesting venereal transmission may occur. Two boars orally inoculated with PEDV showed clinical signs and virus shedding in feces. Transient presence of the PEDV genome was detected by RT-qPCR in the seminal (5.06 × 102 to 2.44 × 103 genomic copies/mL) and sperm-rich fraction of semen (5.64 × 102 to 3.40 × 104 genomic copies/mL) and a longer duration of viral shedding was observed in the sperm-rich fraction. The evidence of PEDV shedding in semen raises new questions in term of disease spread within the pig population with the use of potentially contaminated semen.http://link.springer.com/article/10.1186/s13567-018-0505-2 |
| spellingShingle | Sarah Gallien Angélique Moro Gérald Lediguerher Virginie Catinot Frédéric Paboeuf Lionel Bigault Mustapha Berri Phillip C. Gauger Nathalie Pozzi Edith Authié Nicolas Rose Béatrice Grasland Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars Veterinary Research |
| title | Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars |
| title_full | Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars |
| title_fullStr | Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars |
| title_full_unstemmed | Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars |
| title_short | Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars |
| title_sort | evidence of porcine epidemic diarrhea virus pedv shedding in semen from infected specific pathogen free boars |
| url | http://link.springer.com/article/10.1186/s13567-018-0505-2 |
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