Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces
Wheat leaf rust caused by <i>Puccinia triticina</i> Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (<i>T. turgidum</i> ssp. <i>Dicoccum</i>)<i>,</i> commonly called Khapli wheat in I...
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MDPI AG
2022-07-01
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| author | K. Raghunandan Jatin Tanwar Shivanagouda N. Patil Ajay Kumar Chandra Sandhya Tyagi Priyanka Agarwal Niharika Mallick Niranjana Murukan Jyoti Kumari Tanmaya Kumar Sahu Sherry R. Jacob Atul Kumar Suresh Yadav Sneha Nyamgoud Vinod Amit Kumar Singh Shailendra Kumar Jha |
| author_facet | K. Raghunandan Jatin Tanwar Shivanagouda N. Patil Ajay Kumar Chandra Sandhya Tyagi Priyanka Agarwal Niharika Mallick Niranjana Murukan Jyoti Kumari Tanmaya Kumar Sahu Sherry R. Jacob Atul Kumar Suresh Yadav Sneha Nyamgoud Vinod Amit Kumar Singh Shailendra Kumar Jha |
| author_sort | K. Raghunandan |
| collection | DOAJ |
| description | Wheat leaf rust caused by <i>Puccinia triticina</i> Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (<i>T. turgidum</i> ssp. <i>Dicoccum</i>)<i>,</i> commonly called Khapli wheat in India, is known to have evolved from wild emmer (<i>Triticum turgidum</i> var. <i>dicoccoides</i>), and harbors a good number of leaf rust resistance genes. In the present study, we are reporting on the screening of one hundred and twenty-three dicoccum wheat germplasm accessions against the leaf rust pathotype 77-5. Among these, an average of 45.50% of the germplasms were resistant, 46.74% were susceptible, and 8.53% had mesothetic reactions. Further, selected germplasm lines with accession numbers IC138898, IC47022, IC535116, IC535133, IC535139, IC551396, and IC534144 showed high level of resistance against the eighteen prevalent pathotypes. The infection type varied from “;”, “;N”, “;N1” to “;NC”. PCR-based analysis of the resistant dicoccum lines with SSR marker <i>gwm508</i> linked to the <i>Lr53</i> gene, a leaf rust resistance gene effective against all the prevalent pathotypes of leaf rust in India and identified from a <i>T. turgidum</i> var. <i>dicoccoides</i> germplasm, indicated that <i>Lr53</i> is not present in the selected accessions. Moreover, we have also generated 35K SNP genotyping data of seven lines and the susceptible control, Mandsaur Local, to study their relationships. The GDIRT tool based on homozygous genotypic differences revealed that the seven genotypes are unique to each other and may carry different resistance genes for leaf rust. |
| first_indexed | 2024-03-09T05:05:57Z |
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| language | English |
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| publishDate | 2022-07-01 |
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| spelling | doaj.art-5c491522138c4e4890f3d003b371ea552023-12-03T12:55:06ZengMDPI AGPlants2223-77472022-07-011115196510.3390/plants11151965Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat LandracesK. Raghunandan0Jatin Tanwar1Shivanagouda N. Patil2Ajay Kumar Chandra3Sandhya Tyagi4Priyanka Agarwal5Niharika Mallick6Niranjana Murukan7Jyoti Kumari8Tanmaya Kumar Sahu9Sherry R. Jacob10Atul Kumar11Suresh Yadav12Sneha Nyamgoud13Vinod14Amit Kumar Singh15Shailendra Kumar Jha16Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, IndiaICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, IndiaICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, IndiaDivision of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110008, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaWheat leaf rust caused by <i>Puccinia triticina</i> Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat (<i>T. turgidum</i> ssp. <i>Dicoccum</i>)<i>,</i> commonly called Khapli wheat in India, is known to have evolved from wild emmer (<i>Triticum turgidum</i> var. <i>dicoccoides</i>), and harbors a good number of leaf rust resistance genes. In the present study, we are reporting on the screening of one hundred and twenty-three dicoccum wheat germplasm accessions against the leaf rust pathotype 77-5. Among these, an average of 45.50% of the germplasms were resistant, 46.74% were susceptible, and 8.53% had mesothetic reactions. Further, selected germplasm lines with accession numbers IC138898, IC47022, IC535116, IC535133, IC535139, IC551396, and IC534144 showed high level of resistance against the eighteen prevalent pathotypes. The infection type varied from “;”, “;N”, “;N1” to “;NC”. PCR-based analysis of the resistant dicoccum lines with SSR marker <i>gwm508</i> linked to the <i>Lr53</i> gene, a leaf rust resistance gene effective against all the prevalent pathotypes of leaf rust in India and identified from a <i>T. turgidum</i> var. <i>dicoccoides</i> germplasm, indicated that <i>Lr53</i> is not present in the selected accessions. Moreover, we have also generated 35K SNP genotyping data of seven lines and the susceptible control, Mandsaur Local, to study their relationships. The GDIRT tool based on homozygous genotypic differences revealed that the seven genotypes are unique to each other and may carry different resistance genes for leaf rust.https://www.mdpi.com/2223-7747/11/15/1965leaf rustresistance sourcekhapli landracewheatSNP genotyping |
| spellingShingle | K. Raghunandan Jatin Tanwar Shivanagouda N. Patil Ajay Kumar Chandra Sandhya Tyagi Priyanka Agarwal Niharika Mallick Niranjana Murukan Jyoti Kumari Tanmaya Kumar Sahu Sherry R. Jacob Atul Kumar Suresh Yadav Sneha Nyamgoud Vinod Amit Kumar Singh Shailendra Kumar Jha Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces Plants leaf rust resistance source khapli landrace wheat SNP genotyping |
| title | Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces |
| title_full | Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces |
| title_fullStr | Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces |
| title_full_unstemmed | Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces |
| title_short | Identification of Novel Broad-Spectrum Leaf Rust Resistance Sources from Khapli Wheat Landraces |
| title_sort | identification of novel broad spectrum leaf rust resistance sources from khapli wheat landraces |
| topic | leaf rust resistance source khapli landrace wheat SNP genotyping |
| url | https://www.mdpi.com/2223-7747/11/15/1965 |
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