Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell
The present study preliminarily evaluated neurotoxicity injuries induced by chlorpyrifos in PC12 cell, which were used as a model for nervous cell system. In cultured PC12 cell, application of soy isoflavones (genistein, daidzein, monomer and mixture) significantly reduced chlorpyrifos induced tox...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-03-01
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| Series: | Oil Crop Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2096242820300087 |
| _version_ | 1818352339670532096 |
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| author | Yu Gao Jiajia Xu Menglei Xu Sunsen Shi Jinfeng Xiong |
| author_facet | Yu Gao Jiajia Xu Menglei Xu Sunsen Shi Jinfeng Xiong |
| author_sort | Yu Gao |
| collection | DOAJ |
| description | The present study preliminarily evaluated neurotoxicity injuries induced by chlorpyrifos in PC12 cell, which were used as a model for nervous cell system. In cultured PC12 cell, application of soy isoflavones (genistein, daidzein, monomer and mixture) significantly reduced chlorpyrifos induced toxicity, a widely used pesticide, and resulted in a better cell survival rate. Treatments with isoflavones reduced malondialdehyde content, reactive oxygengeneration and acetylcholine level in medium, and maintained mitochondrial membrane potential integrity. Daidzein enhanced endogenous antioxidant system in PC12 cell with an increasing in superoxide dismutase per-unit activity. Genistein reduced acetylcholine content in the medium. Daidzein and genistein showed different effects, and their combined effect were greater than individual. In conclusion, soy isoflavones as an antioxidant and neuroprotectant, enhanced choline metabolism, which effectively mitigated disadvantageous influence of PC12 cell caused by chlorpyrifos. |
| first_indexed | 2024-12-13T18:52:04Z |
| format | Article |
| id | doaj.art-5c49280c5dd841ebb7e0d72e8b7118d6 |
| institution | Directory Open Access Journal |
| issn | 2096-2428 |
| language | English |
| last_indexed | 2024-12-13T18:52:04Z |
| publishDate | 2020-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Oil Crop Science |
| spelling | doaj.art-5c49280c5dd841ebb7e0d72e8b7118d62022-12-21T23:34:55ZengKeAi Communications Co., Ltd.Oil Crop Science2096-24282020-03-015115Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cellYu Gao0Jiajia Xu1Menglei Xu2Sunsen Shi3Jinfeng Xiong4College of Plant Protection, Jilin Agricultural University, Changchun, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun, ChinaCollege of Food Science and Engineering/State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China; Corresponding author.College of Plant Protection, Jilin Agricultural University, Changchun, ChinaChangchun Institute of Biological Products, Changchun, ChinaThe present study preliminarily evaluated neurotoxicity injuries induced by chlorpyrifos in PC12 cell, which were used as a model for nervous cell system. In cultured PC12 cell, application of soy isoflavones (genistein, daidzein, monomer and mixture) significantly reduced chlorpyrifos induced toxicity, a widely used pesticide, and resulted in a better cell survival rate. Treatments with isoflavones reduced malondialdehyde content, reactive oxygengeneration and acetylcholine level in medium, and maintained mitochondrial membrane potential integrity. Daidzein enhanced endogenous antioxidant system in PC12 cell with an increasing in superoxide dismutase per-unit activity. Genistein reduced acetylcholine content in the medium. Daidzein and genistein showed different effects, and their combined effect were greater than individual. In conclusion, soy isoflavones as an antioxidant and neuroprotectant, enhanced choline metabolism, which effectively mitigated disadvantageous influence of PC12 cell caused by chlorpyrifos.http://www.sciencedirect.com/science/article/pii/S2096242820300087ChlorpyrifosSoy isoflavoneGenisteinDaidzeinPC12 cellCytotoxicity |
| spellingShingle | Yu Gao Jiajia Xu Menglei Xu Sunsen Shi Jinfeng Xiong Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell Oil Crop Science Chlorpyrifos Soy isoflavone Genistein Daidzein PC12 cell Cytotoxicity |
| title | Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell |
| title_full | Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell |
| title_fullStr | Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell |
| title_full_unstemmed | Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell |
| title_short | Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell |
| title_sort | genistein and daidzein reduced chlorpyrifos induced damage in pc12 cell |
| topic | Chlorpyrifos Soy isoflavone Genistein Daidzein PC12 cell Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2096242820300087 |
| work_keys_str_mv | AT yugao genisteinanddaidzeinreducedchlorpyrifosinduceddamageinpc12cell AT jiajiaxu genisteinanddaidzeinreducedchlorpyrifosinduceddamageinpc12cell AT mengleixu genisteinanddaidzeinreducedchlorpyrifosinduceddamageinpc12cell AT sunsenshi genisteinanddaidzeinreducedchlorpyrifosinduceddamageinpc12cell AT jinfengxiong genisteinanddaidzeinreducedchlorpyrifosinduceddamageinpc12cell |