False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors
The role of phosphatidylethanol (PEth) as an alcohol consumption marker is increasing in clinical and forensic medicine. During the COVID-19 pandemic, the role of hygiene increased, and it became common practice to use disinfectants almost everywhere. This paper highlights a possible source of false...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2297-8739/9/9/250 |
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| author | Anton Bashilov Sergey Osipenko Karolina Ikonnikova Oxana Kovaleva Boris Izotov Evgeny Nikolaev Yury Kostyukevich |
| author_facet | Anton Bashilov Sergey Osipenko Karolina Ikonnikova Oxana Kovaleva Boris Izotov Evgeny Nikolaev Yury Kostyukevich |
| author_sort | Anton Bashilov |
| collection | DOAJ |
| description | The role of phosphatidylethanol (PEth) as an alcohol consumption marker is increasing in clinical and forensic medicine. During the COVID-19 pandemic, the role of hygiene increased, and it became common practice to use disinfectants almost everywhere. This paper highlights a possible source of false positive results (by the vapors of alcohols during the blood spot drying process) in dried blood spots (DBS) by LC-MS/MS quantitation of PEth. To achieve this, the PEth quantitation method was validated according to FDA guidelines. Additionally, the synthesis of phosphatidyl derivatives by phospholipase D (PLD) in the presence of methanol and 2-propanol vapors during the DBS process was determined. Each PEth-negative sample from a healthy male patient incubated in the presence of ethanol vapor becomes PEth-positive. After 4 h of DBS drying, teetotalers become “moderate drinkers”. It is necessary to avoid using alcohol-containing disinfectants in treatment rooms, where DBS is sampled. |
| first_indexed | 2024-03-09T22:31:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T22:31:49Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-5c496366cec645b5807787a1e679be6b2023-11-23T18:54:46ZengMDPI AGSeparations2297-87392022-09-019925010.3390/separations9090250False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol VaporsAnton Bashilov0Sergey Osipenko1Karolina Ikonnikova2Oxana Kovaleva3Boris Izotov4Evgeny Nikolaev5Yury Kostyukevich6Skolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, RussiaSkolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, RussiaN.V. Sklifosovskiy Institute of Clinical Medicine, First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaSkolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, RussiaInstitute for Translational Medicine and Biotechnology, First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaSkolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, RussiaSkolkovo Institute of Science and Technology, Nobel Str., 3, 121205 Moscow, RussiaThe role of phosphatidylethanol (PEth) as an alcohol consumption marker is increasing in clinical and forensic medicine. During the COVID-19 pandemic, the role of hygiene increased, and it became common practice to use disinfectants almost everywhere. This paper highlights a possible source of false positive results (by the vapors of alcohols during the blood spot drying process) in dried blood spots (DBS) by LC-MS/MS quantitation of PEth. To achieve this, the PEth quantitation method was validated according to FDA guidelines. Additionally, the synthesis of phosphatidyl derivatives by phospholipase D (PLD) in the presence of methanol and 2-propanol vapors during the DBS process was determined. Each PEth-negative sample from a healthy male patient incubated in the presence of ethanol vapor becomes PEth-positive. After 4 h of DBS drying, teetotalers become “moderate drinkers”. It is necessary to avoid using alcohol-containing disinfectants in treatment rooms, where DBS is sampled.https://www.mdpi.com/2297-8739/9/9/250phosphatidylethanolalcohol biomarkerforensicsclinical medicineLC-MS/MS |
| spellingShingle | Anton Bashilov Sergey Osipenko Karolina Ikonnikova Oxana Kovaleva Boris Izotov Evgeny Nikolaev Yury Kostyukevich False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors Separations phosphatidylethanol alcohol biomarker forensics clinical medicine LC-MS/MS |
| title | False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors |
| title_full | False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors |
| title_fullStr | False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors |
| title_full_unstemmed | False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors |
| title_short | False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors |
| title_sort | false positive results of phosphatidylethanol peth quantitation in dried blood spots dbs the influence of alcohol vapors |
| topic | phosphatidylethanol alcohol biomarker forensics clinical medicine LC-MS/MS |
| url | https://www.mdpi.com/2297-8739/9/9/250 |
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