The increasing of enamel calcium level after casein phosphopeptideamorphous calcium phosphate covering
<p><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: norm...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2012-06-01
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| Series: | Dental Journal |
| Subjects: | |
| Online Access: | http://e-journal.unair.ac.id/index.php/MKG/article/view/830 |
| Summary: | <p><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Caries process is characterized by the presence of demineralization. Demineralization is caused by organic acids as a </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>result of carbohydrate substrate fermentation. Remineralization is a natural repair process for non-cavitated lesions. Remineralization </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>occurs if there are Ca</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2+ </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and PO</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>43- </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ions in sufficient quantities. Casein-amorphous calcium phosphate phosphopeptide (CPP-ACP) is a </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>paste material containing milk protein (casein), that actually contains minerals, such as calcium and phosphate. The casein ability to </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>stabilize calcium phosphate and enhance mineral solubility and bioavailability confers upon CPP potential to be biological delivery </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>vehicles for calcium and phosphate. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine the calcium levels in tooth enamel after being </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>covered with CPP-ACP 2 times a day for 3, 14 and 28 days. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sample were bovine incisors of 3 year old cows divided into </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>4 groups, namely group I as control group, group II, III and IV as treatment groups covered with CPP-ACP 2 times a day. All of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>those teeth were then immersed in artificial saliva. Group II was immersed for 3 days, while group III was immersed for 14 days, and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>group IV was immersed for 28 days. One drop of CPP-ACP was used to cover the entire labial surface of teeth. The measurement of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the calcium levels was then conducted by using titration method. All data were analyzed by One- Way ANOVA test with 5% degree of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>confidence. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The results showed significant difference of the calcium levels in tooth enamel of those groups after covered with </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>CPP-ACP 2 times a day for 3, 14 and 28 days (p = 0.001). There is also significant difference of the calcium levels in tooth enamel of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>those treatment groups and the control group (p = 0.001). </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The calcium levels of tooth enamel are increased after covered </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with CPP-ACP 2 times a day for 3, 14 and 28 days.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Proses terjadinya karies gigi ditandai oleh adanya demineralisasi. Demineralisasi terjadi oleh asam organik </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sebagai hasil fermentasi substrat karbohidrat oleh bakteri. Remineralisasi adalah proses perbaikan alami untuk lesi non cavitated. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Remineralisasi terjadi jika terdapat ion Ca</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2+ </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dan PO</em><span style="font-family: TribuneItalic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>43- </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dalam jumlah cukup. Casein phosphopeptide-amorphous calcium phosphate </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(CPP-ACP) adalah bahan berbentuk pasta berisi suatu protein susu (kasein). Pada kasein terkandung mineral kalsium dan fosfat. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Kemampuan kasein untuk menstabilkan kalsium fosfat dan meningkatkan kelarutan dan bioavailabilitas sehingga CPP memiliki </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>potensi menghantarkan kalsium dan fosfat. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Tujuan penelitian ini untuk mengetahui kadar kalsium enamel setelah pengulasan </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>CPP-ACP pada permukaan enamel 2 kali sehari selama 3,14 dan 28 hari. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sampel adalah gigi insisif sapi, usia 3 tahun. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Pada penelitian ini sampel (n = 24) dibagi menjadi 4 kelompok yaitu kelompok I sebagai kelompok kontrol, dan kelompok II, III, IV </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sebagai kelompok perlakuan dengan pengulasan CPP-ACP 2 kali sehari. Semua kelompok direndam dalam saliva buatan. Kelompok </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>II direndam 3 hari, kelompok III direndam 14 hari, kelompok IV direndam 28 hari. Pengulasan CPP-ACP sejumlah 1 tetes diratakan </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>pada seluruh permukaan labial gigi. Pengukuran kadar kalsium dilakukan dengan metode titrasi. Semua data dianalisa dengan uji </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>One-Way ANOVA dengan taraf kemaknaan 5%. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Hasil penelitian menunjukkan perbedaan yang signifikan kadar kalsium enamel </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>gigi di antara kelompok (p = 0,001). Hasil penelitian menunjukkan perbedaan yang signifikan kadar kalsium gigi pada kelompok</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> <span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kontrol dan perlakuan antara lama waktu pengulasan 3, 14, dan 28 hari (p = 0,001). </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Kesimpulan: </strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Pengulasan CPP-ACP selama 3, </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>14 dan 28 hari mampu meningkatkan kadar kalsium pada enamel.</em></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p> |
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| ISSN: | 1978-3728 2442-9740 |