Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus
DUF579 (domain unknown function 579) genes have been reported to play diverse roles in cell wall biosynthesis, such as in glucuronoxylan (GX) synthesis. As GX is a major type of hemicelluloses in hard wood species, how DUF579 genes function in wood formation remains to be demonstrated in planta. Thi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00493/full |
| _version_ | 1818056991504859136 |
|---|---|
| author | Dongliang eSong Jinshan eGui Chenchen eLiu Jiayan eSun Laigeng eLi |
| author_facet | Dongliang eSong Jinshan eGui Chenchen eLiu Jiayan eSun Laigeng eLi |
| author_sort | Dongliang eSong |
| collection | DOAJ |
| description | DUF579 (domain unknown function 579) genes have been reported to play diverse roles in cell wall biosynthesis, such as in glucuronoxylan (GX) synthesis. As GX is a major type of hemicelluloses in hard wood species, how DUF579 genes function in wood formation remains to be demonstrated in planta. This study reports a Populus DUF579 gene, PtrDUF579-3, which is characterized for its function in wood cell wall formation. PtrDUF579-3 is localized in Golgi apparatus and catalyzes methylation of the glucuronic acid (GlcA) in GX biosynthesis. Suppression of PtrDUF579-3 expression in Populus caused a reduction in both the GlcA side chain number and GlcA side chain methylation on the GX backbone. The modified GX polymer through PtrDUF579-3 suppression was more susceptible to acid treatment and the PtrDUF579-3 suppressed plants displayed enhanced cellulose digestibility. These results suggest that PtrDUF579-3 is involved in GX biosynthesis and GX structure can be modified through PtrDUF579-3 suppression in Populus. |
| first_indexed | 2024-12-10T12:37:38Z |
| format | Article |
| id | doaj.art-5c50c958d4a541eba3f493dfc445d0ea |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T12:37:38Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5c50c958d4a541eba3f493dfc445d0ea2022-12-22T01:48:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-04-01710.3389/fpls.2016.00493183928Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in PopulusDongliang eSong0Jinshan eGui1Chenchen eLiu2Jiayan eSun3Laigeng eLi4National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesDUF579 (domain unknown function 579) genes have been reported to play diverse roles in cell wall biosynthesis, such as in glucuronoxylan (GX) synthesis. As GX is a major type of hemicelluloses in hard wood species, how DUF579 genes function in wood formation remains to be demonstrated in planta. This study reports a Populus DUF579 gene, PtrDUF579-3, which is characterized for its function in wood cell wall formation. PtrDUF579-3 is localized in Golgi apparatus and catalyzes methylation of the glucuronic acid (GlcA) in GX biosynthesis. Suppression of PtrDUF579-3 expression in Populus caused a reduction in both the GlcA side chain number and GlcA side chain methylation on the GX backbone. The modified GX polymer through PtrDUF579-3 suppression was more susceptible to acid treatment and the PtrDUF579-3 suppressed plants displayed enhanced cellulose digestibility. These results suggest that PtrDUF579-3 is involved in GX biosynthesis and GX structure can be modified through PtrDUF579-3 suppression in Populus.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00493/fullPopulusWood engineeringGlucuronoxylanDUF579glucuronic acid methyltransferases |
| spellingShingle | Dongliang eSong Jinshan eGui Chenchen eLiu Jiayan eSun Laigeng eLi Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus Frontiers in Plant Science Populus Wood engineering Glucuronoxylan DUF579 glucuronic acid methyltransferases |
| title | Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus |
| title_full | Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus |
| title_fullStr | Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus |
| title_full_unstemmed | Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus |
| title_short | Suppression of PtrDUF579-3 expression causes structural changes of the glucuronoxylan in Populus |
| title_sort | suppression of ptrduf579 3 expression causes structural changes of the glucuronoxylan in populus |
| topic | Populus Wood engineering Glucuronoxylan DUF579 glucuronic acid methyltransferases |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00493/full |
| work_keys_str_mv | AT dongliangesong suppressionofptrduf5793expressioncausesstructuralchangesoftheglucuronoxylaninpopulus AT jinshanegui suppressionofptrduf5793expressioncausesstructuralchangesoftheglucuronoxylaninpopulus AT chencheneliu suppressionofptrduf5793expressioncausesstructuralchangesoftheglucuronoxylaninpopulus AT jiayanesun suppressionofptrduf5793expressioncausesstructuralchangesoftheglucuronoxylaninpopulus AT laigengeli suppressionofptrduf5793expressioncausesstructuralchangesoftheglucuronoxylaninpopulus |