A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

Abstract This study describes a simple method for the large‐scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were colle...

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Bibliographic Details
Main Authors: Saeed El‐Ashram, Yu Zhang, Yongsheng Ji, Dina Salama, Kun Mei, Li Zhili, Huang Shujian, Haoji Zhang, Shawky M. Aboelhadid, Reem A. Alajmi, Dina M. Metwally, Manal F. El‐Khadragy, Billy M. Hargis, Guillermo Tellez‐Isaias, Beniamino T. Cenci‐Goga, Musafiri Karama, Munyaradzi C. Marufu, Fathi Abouhajer, Gamal Ali Abdelhafez Hamady, Abeer El Wakil, Ibrahim Al Nasr, Xun Suo
Format: Article
Language:English
Published: Wiley 2021-03-01
Series:Veterinary Medicine and Science
Subjects:
flow cytometry
purification
T. gondii tachyzoites and bradyzoites
trypsin
taurodeoxycholic acid
Online Access:https://doi.org/10.1002/vms3.364

Internet

https://doi.org/10.1002/vms3.364

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