Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production
Abstract Plant-sourced aromatic amino acid (AAA) derivatives are a vast group of compounds with broad applications. Here, we present the development of a yeast consortium for efficient production of (S)-norcoclaurine, the key precursor for benzylisoquinoline alkaloid biosynthesis. A xylose transport...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43049-w |
| _version_ | 1797414721922531328 |
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| author | Meirong Gao Yuxin Zhao Zhanyi Yao Qianhe Su Payton Van Beek Zengyi Shao |
| author_facet | Meirong Gao Yuxin Zhao Zhanyi Yao Qianhe Su Payton Van Beek Zengyi Shao |
| author_sort | Meirong Gao |
| collection | DOAJ |
| description | Abstract Plant-sourced aromatic amino acid (AAA) derivatives are a vast group of compounds with broad applications. Here, we present the development of a yeast consortium for efficient production of (S)-norcoclaurine, the key precursor for benzylisoquinoline alkaloid biosynthesis. A xylose transporter enables the concurrent mixed-sugar utilization in Scheffersomyces stipitis, which plays a crucial role in enhancing the flux entering the highly regulated shikimate pathway located upstream of AAA biosynthesis. Two quinate permeases isolated from Aspergillus niger facilitates shikimate translocation to the co-cultured Saccharomyces cerevisiae that converts shikimate to (S)-norcoclaurine, resulting in the maximal titer (11.5 mg/L), nearly 110-fold higher than the titer reported for an S. cerevisiae monoculture. Our findings magnify the potential of microbial consortium platforms for the economical de novo synthesis of complex compounds, where pathway modularization and compartmentalization in distinct specialty strains enable effective fine-tuning of long biosynthetic pathways and diminish intermediate buildup, thereby leading to increases in production. |
| first_indexed | 2024-03-09T05:37:41Z |
| format | Article |
| id | doaj.art-5c632afb5e39424da3430f63a655bdcf |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-09T05:37:41Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5c632afb5e39424da3430f63a655bdcf2023-12-03T12:27:19ZengNature PortfolioNature Communications2041-17232023-11-0114111310.1038/s41467-023-43049-wXylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid productionMeirong Gao0Yuxin Zhao1Zhanyi Yao2Qianhe Su3Payton Van Beek4Zengyi Shao5Department of Chemical and Biological Engineering, Iowa State UniversityDepartment of Chemical and Biological Engineering, Iowa State UniversityDepartment of Chemical and Biological Engineering, Iowa State UniversityDepartment of Chemical and Biological Engineering, Iowa State UniversityDepartment of Chemical and Biological Engineering, Iowa State UniversityDepartment of Chemical and Biological Engineering, Iowa State UniversityAbstract Plant-sourced aromatic amino acid (AAA) derivatives are a vast group of compounds with broad applications. Here, we present the development of a yeast consortium for efficient production of (S)-norcoclaurine, the key precursor for benzylisoquinoline alkaloid biosynthesis. A xylose transporter enables the concurrent mixed-sugar utilization in Scheffersomyces stipitis, which plays a crucial role in enhancing the flux entering the highly regulated shikimate pathway located upstream of AAA biosynthesis. Two quinate permeases isolated from Aspergillus niger facilitates shikimate translocation to the co-cultured Saccharomyces cerevisiae that converts shikimate to (S)-norcoclaurine, resulting in the maximal titer (11.5 mg/L), nearly 110-fold higher than the titer reported for an S. cerevisiae monoculture. Our findings magnify the potential of microbial consortium platforms for the economical de novo synthesis of complex compounds, where pathway modularization and compartmentalization in distinct specialty strains enable effective fine-tuning of long biosynthetic pathways and diminish intermediate buildup, thereby leading to increases in production.https://doi.org/10.1038/s41467-023-43049-w |
| spellingShingle | Meirong Gao Yuxin Zhao Zhanyi Yao Qianhe Su Payton Van Beek Zengyi Shao Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production Nature Communications |
| title | Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| title_full | Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| title_fullStr | Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| title_full_unstemmed | Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| title_short | Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| title_sort | xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production |
| url | https://doi.org/10.1038/s41467-023-43049-w |
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