The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle
Background: Salmonella enteritidis causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate S. enteritidis from cattle feces samples and also to evaluate the frequency of genes as...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2020-05-01
|
| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/14671 |
| _version_ | 1819241195174363136 |
|---|---|
| author | Reza RANJBAR Farhad SAFARPOOR DEHKORDI Mohammad HEIAT |
| author_facet | Reza RANJBAR Farhad SAFARPOOR DEHKORDI Mohammad HEIAT |
| author_sort | Reza RANJBAR |
| collection | DOAJ |
| description | Background: Salmonella enteritidis causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate S. enteritidis from cattle feces samples and also to evaluate the frequency of genes associated with multi-drug resistance (MDR).
Methods: One hundred ten fecal samples of cattle were collected from Jul to Dec, 2017 in Khuzestan Province, southern Iran. Bacterial culture and molecular methods were used to isolate and identify S. enteritidis. Disk diffusion method was used to assess antibiotic susceptibility. Then Polymerase chain reaction (PCR), assay was used for definitive diagnosis of S. enteritidis and resistance genes.
Results: Overall, 101 (91.81%) samples were detected to be contaminated with Salmonella genus and 86 samples (85.14%) were identified as S. enteritidis. The highest and lowest antibiotic resistance were belonged to gentamicin (n=70, 81.39%), and tetracycline (n=6, 6.97%). Besides, 64 samples (74.42%) had 2-10 drugs resistance patterns. Moreover, the highest and the least resistance were related to blaIMP-1 (n=73, 84.88%) and tet(B) (n=49, 56.97%) genes respectively.
Conclusion: The drug-induced genes in S. enteritidis have a high frequency. Therefore, antibiotic resistance and high MDR to antibiotics can be due to the incorrect use of antibiotics and the lack of health monitoring in Cattle farms. |
| first_indexed | 2024-12-23T14:20:03Z |
| format | Article |
| id | doaj.art-5c635802d56444409a05e92b14430736 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-23T14:20:03Z |
| publishDate | 2020-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-5c635802d56444409a05e92b144307362022-12-21T17:43:49ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932020-05-01495The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from CattleReza RANJBAR0Farhad SAFARPOOR DEHKORDI1Mohammad HEIAT2Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, IranHalal Research Center of IRI, FDA, Tehran, IranBaqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: Salmonella enteritidis causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate S. enteritidis from cattle feces samples and also to evaluate the frequency of genes associated with multi-drug resistance (MDR). Methods: One hundred ten fecal samples of cattle were collected from Jul to Dec, 2017 in Khuzestan Province, southern Iran. Bacterial culture and molecular methods were used to isolate and identify S. enteritidis. Disk diffusion method was used to assess antibiotic susceptibility. Then Polymerase chain reaction (PCR), assay was used for definitive diagnosis of S. enteritidis and resistance genes. Results: Overall, 101 (91.81%) samples were detected to be contaminated with Salmonella genus and 86 samples (85.14%) were identified as S. enteritidis. The highest and lowest antibiotic resistance were belonged to gentamicin (n=70, 81.39%), and tetracycline (n=6, 6.97%). Besides, 64 samples (74.42%) had 2-10 drugs resistance patterns. Moreover, the highest and the least resistance were related to blaIMP-1 (n=73, 84.88%) and tet(B) (n=49, 56.97%) genes respectively. Conclusion: The drug-induced genes in S. enteritidis have a high frequency. Therefore, antibiotic resistance and high MDR to antibiotics can be due to the incorrect use of antibiotics and the lack of health monitoring in Cattle farms.https://ijph.tums.ac.ir/index.php/ijph/article/view/14671Salmonella enteritidisResistance gene;Multi-drug resistance;Cattle |
| spellingShingle | Reza RANJBAR Farhad SAFARPOOR DEHKORDI Mohammad HEIAT The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle Iranian Journal of Public Health Salmonella enteritidis Resistance gene; Multi-drug resistance; Cattle |
| title | The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle |
| title_full | The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle |
| title_fullStr | The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle |
| title_full_unstemmed | The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle |
| title_short | The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle |
| title_sort | frequency of resistance genes in salmonella enteritidis strains isolated from cattle |
| topic | Salmonella enteritidis Resistance gene; Multi-drug resistance; Cattle |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/14671 |
| work_keys_str_mv | AT rezaranjbar thefrequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle AT farhadsafarpoordehkordi thefrequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle AT mohammadheiat thefrequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle AT rezaranjbar frequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle AT farhadsafarpoordehkordi frequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle AT mohammadheiat frequencyofresistancegenesinsalmonellaenteritidisstrainsisolatedfromcattle |