The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence
The retinoblastoma Rb protein is an important factor controlling the cell cycle. Yet, mammalian cells carrying Rb deletions are still able to arrest under growth-limiting conditions. The Rb-related proteins p107 and p130, which are components of the DREAM complex, had been suggested to be responsibl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/26876 |
| _version_ | 1811181034033643520 |
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| author | Christina FS Mages Axel Wintsche Stephan H Bernhart Gerd A Müller |
| author_facet | Christina FS Mages Axel Wintsche Stephan H Bernhart Gerd A Müller |
| author_sort | Christina FS Mages |
| collection | DOAJ |
| description | The retinoblastoma Rb protein is an important factor controlling the cell cycle. Yet, mammalian cells carrying Rb deletions are still able to arrest under growth-limiting conditions. The Rb-related proteins p107 and p130, which are components of the DREAM complex, had been suggested to be responsible for a continued ability to arrest by inhibiting E2f activity and by recruiting chromatin-modifying enzymes. Here, we show that p130 and p107 are not sufficient for DREAM-dependent repression. We identify the MuvB protein Lin37 as an essential factor for DREAM function. Cells not expressing Lin37 proliferate normally, but DREAM completely loses its ability to repress genes in G0/G1 while all remaining subunits, including p130/p107, still bind to target gene promoters. Furthermore, cells lacking both Rb and Lin37 are incapable of exiting the cell cycle. Thus, Lin37 is an essential component of DREAM that cooperates with Rb to induce quiescence. |
| first_indexed | 2024-04-11T09:12:34Z |
| format | Article |
| id | doaj.art-5c65d5f39df14de381dfeb5ed319065b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:34Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5c65d5f39df14de381dfeb5ed319065b2022-12-22T04:32:28ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.26876The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescenceChristina FS Mages0https://orcid.org/0000-0002-2643-7265Axel Wintsche1Stephan H Bernhart2Gerd A Müller3https://orcid.org/0000-0002-4967-2487Molecular Oncology, Medical School, University of Leipzig, Leipzig, GermanyMolecular Oncology, Medical School, University of Leipzig, Leipzig, Germany; Computational EvoDevo Group, Department of Computer Science and Interdisciplinary Center for Bioinformatics, University of Leipzig, Leipzig, GermanyTranscriptome Bioinformatics Group, Department of Computer Science, University of Leipzig, Leipzig, Germany; Interdisciplinary Center for Bioinformatics, University of Leipzig, Leipzig, GermanyMolecular Oncology, Medical School, University of Leipzig, Leipzig, GermanyThe retinoblastoma Rb protein is an important factor controlling the cell cycle. Yet, mammalian cells carrying Rb deletions are still able to arrest under growth-limiting conditions. The Rb-related proteins p107 and p130, which are components of the DREAM complex, had been suggested to be responsible for a continued ability to arrest by inhibiting E2f activity and by recruiting chromatin-modifying enzymes. Here, we show that p130 and p107 are not sufficient for DREAM-dependent repression. We identify the MuvB protein Lin37 as an essential factor for DREAM function. Cells not expressing Lin37 proliferate normally, but DREAM completely loses its ability to repress genes in G0/G1 while all remaining subunits, including p130/p107, still bind to target gene promoters. Furthermore, cells lacking both Rb and Lin37 are incapable of exiting the cell cycle. Thus, Lin37 is an essential component of DREAM that cooperates with Rb to induce quiescence.https://elifesciences.org/articles/26876cell cycle regulationtranscription factorsgene expressioncell cycle exitcell proliferationcancer |
| spellingShingle | Christina FS Mages Axel Wintsche Stephan H Bernhart Gerd A Müller The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence eLife cell cycle regulation transcription factors gene expression cell cycle exit cell proliferation cancer |
| title | The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence |
| title_full | The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence |
| title_fullStr | The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence |
| title_full_unstemmed | The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence |
| title_short | The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence |
| title_sort | dream complex through its subunit lin37 cooperates with rb to initiate quiescence |
| topic | cell cycle regulation transcription factors gene expression cell cycle exit cell proliferation cancer |
| url | https://elifesciences.org/articles/26876 |
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