Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames.
BACKGROUND: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could b...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2918503?pdf=render |
| _version_ | 1818134015424593920 |
|---|---|
| author | Joern W Kuhbier Christina Allmeling Kerstin Reimers Anja Hillmer Cornelia Kasper Bjoern Menger Gudrun Brandes Merlin Guggenheim Peter M Vogt |
| author_facet | Joern W Kuhbier Christina Allmeling Kerstin Reimers Anja Hillmer Cornelia Kasper Bjoern Menger Gudrun Brandes Merlin Guggenheim Peter M Vogt |
| author_sort | Joern W Kuhbier |
| collection | DOAJ |
| description | BACKGROUND: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature. METHODOLOGY/PRINCIPAL FINDINGS: Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen- and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p<0.01). CONCLUSION/SIGNIFICANCE: Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties. We could show adhesion mechanics on intracellular level. Additionally, proliferation kinetics were higher than in enzymatically digested controls, indicating that spider silk does not require modification. Recent findings concerning reduction of cell proliferation after exposure could not be met. As biotechnological production of the hierarchical composition of native spider silk fibres is still a challenge, our study has a pioneer role in researching cellular mechanics on native spider silk fibres. |
| first_indexed | 2024-12-11T09:01:54Z |
| format | Article |
| id | doaj.art-5c6bf50092124a39a7839239685d252e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:01:54Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5c6bf50092124a39a7839239685d252e2022-12-22T01:13:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0158e1203210.1371/journal.pone.0012032Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames.Joern W KuhbierChristina AllmelingKerstin ReimersAnja HillmerCornelia KasperBjoern MengerGudrun BrandesMerlin GuggenheimPeter M VogtBACKGROUND: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature. METHODOLOGY/PRINCIPAL FINDINGS: Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen- and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p<0.01). CONCLUSION/SIGNIFICANCE: Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties. We could show adhesion mechanics on intracellular level. Additionally, proliferation kinetics were higher than in enzymatically digested controls, indicating that spider silk does not require modification. Recent findings concerning reduction of cell proliferation after exposure could not be met. As biotechnological production of the hierarchical composition of native spider silk fibres is still a challenge, our study has a pioneer role in researching cellular mechanics on native spider silk fibres.http://europepmc.org/articles/PMC2918503?pdf=render |
| spellingShingle | Joern W Kuhbier Christina Allmeling Kerstin Reimers Anja Hillmer Cornelia Kasper Bjoern Menger Gudrun Brandes Merlin Guggenheim Peter M Vogt Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. PLoS ONE |
| title | Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. |
| title_full | Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. |
| title_fullStr | Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. |
| title_full_unstemmed | Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. |
| title_short | Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames. |
| title_sort | interactions between spider silk and cells nih 3t3 fibroblasts seeded on miniature weaving frames |
| url | http://europepmc.org/articles/PMC2918503?pdf=render |
| work_keys_str_mv | AT joernwkuhbier interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT christinaallmeling interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT kerstinreimers interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT anjahillmer interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT corneliakasper interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT bjoernmenger interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT gudrunbrandes interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT merlinguggenheim interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes AT petermvogt interactionsbetweenspidersilkandcellsnih3t3fibroblastsseededonminiatureweavingframes |