Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions
Fermentable oligo-, di-, monosaccharides and polyols (FODMAPs) have emerged as key contributors to digestive discomfort and intolerance to certain vegetables, fruits, and plant-based foods. Although strategies exist to minimize FODMAP consumption and exposure, exogenous enzyme supplementation target...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1129329/full |
| _version_ | 1797819678626676736 |
|---|---|
| author | Justin L. Guice Morgan D. Hollins James G. Farmar Kelly M. Tinker Sean M. Garvey |
| author_facet | Justin L. Guice Morgan D. Hollins James G. Farmar Kelly M. Tinker Sean M. Garvey |
| author_sort | Justin L. Guice |
| collection | DOAJ |
| description | Fermentable oligo-, di-, monosaccharides and polyols (FODMAPs) have emerged as key contributors to digestive discomfort and intolerance to certain vegetables, fruits, and plant-based foods. Although strategies exist to minimize FODMAP consumption and exposure, exogenous enzyme supplementation targeting the fructan-type FODMAPs has been underexploited. The objective of this study was to test the hydrolytic efficacy of a food-grade, non-genetically engineered microbial inulinase preparation toward inulin-type fructans in the INFOGEST in vitro static simulation of gastrointestinal (GI) digestion. Purified inulin was shown to undergo acid-mediated hydrolysis at high gastric acidity as well as predominantly inulinase-mediated hydrolysis at lower gastric acidity. Inulinase dose-response simulations of inulin, garlic, and high-fructan meal digestion in the gastric phase suggest that as little as 50 inulinase units (INU) and up to 800 INU per serving promote fructan hydrolysis better than the control simulations without inulinase. Liquid chromatography-mass spectrometry (LC-MS) profiling of fructo-oligosaccharides (FOS) in the gastric digestas following inulinase treatment confirms the fructolytic activity of inulinase under simulated digestive conditions. Altogether, these in vitro digestion data support the use of microbial inulinase as an exogenous enzyme supplement for reducing dietary fructan-type FODMAP exposure. |
| first_indexed | 2024-03-13T09:26:08Z |
| format | Article |
| id | doaj.art-5c6e7a853dc647e5af195e5cc9dcdaeb |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-13T09:26:08Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-5c6e7a853dc647e5af195e5cc9dcdaeb2023-05-26T09:33:58ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-05-011010.3389/fnut.2023.11293291129329Microbial inulinase promotes fructan hydrolysis under simulated gastric conditionsJustin L. GuiceMorgan D. HollinsJames G. FarmarKelly M. TinkerSean M. GarveyFermentable oligo-, di-, monosaccharides and polyols (FODMAPs) have emerged as key contributors to digestive discomfort and intolerance to certain vegetables, fruits, and plant-based foods. Although strategies exist to minimize FODMAP consumption and exposure, exogenous enzyme supplementation targeting the fructan-type FODMAPs has been underexploited. The objective of this study was to test the hydrolytic efficacy of a food-grade, non-genetically engineered microbial inulinase preparation toward inulin-type fructans in the INFOGEST in vitro static simulation of gastrointestinal (GI) digestion. Purified inulin was shown to undergo acid-mediated hydrolysis at high gastric acidity as well as predominantly inulinase-mediated hydrolysis at lower gastric acidity. Inulinase dose-response simulations of inulin, garlic, and high-fructan meal digestion in the gastric phase suggest that as little as 50 inulinase units (INU) and up to 800 INU per serving promote fructan hydrolysis better than the control simulations without inulinase. Liquid chromatography-mass spectrometry (LC-MS) profiling of fructo-oligosaccharides (FOS) in the gastric digestas following inulinase treatment confirms the fructolytic activity of inulinase under simulated digestive conditions. Altogether, these in vitro digestion data support the use of microbial inulinase as an exogenous enzyme supplement for reducing dietary fructan-type FODMAP exposure.https://www.frontiersin.org/articles/10.3389/fnut.2023.1129329/fulldigestionenzymeFODMAPfructanINFOGESTinulin |
| spellingShingle | Justin L. Guice Morgan D. Hollins James G. Farmar Kelly M. Tinker Sean M. Garvey Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions Frontiers in Nutrition digestion enzyme FODMAP fructan INFOGEST inulin |
| title | Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| title_full | Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| title_fullStr | Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| title_full_unstemmed | Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| title_short | Microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| title_sort | microbial inulinase promotes fructan hydrolysis under simulated gastric conditions |
| topic | digestion enzyme FODMAP fructan INFOGEST inulin |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1129329/full |
| work_keys_str_mv | AT justinlguice microbialinulinasepromotesfructanhydrolysisundersimulatedgastricconditions AT morgandhollins microbialinulinasepromotesfructanhydrolysisundersimulatedgastricconditions AT jamesgfarmar microbialinulinasepromotesfructanhydrolysisundersimulatedgastricconditions AT kellymtinker microbialinulinasepromotesfructanhydrolysisundersimulatedgastricconditions AT seanmgarvey microbialinulinasepromotesfructanhydrolysisundersimulatedgastricconditions |