Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex
Abstract Ethanol is a principal ingredient of alcoholic beverages with potential neurotoxicity and genotoxicity, and the ethanol‐associated oxidative DNA damage in the central nervous system is well documented. Natural product may offer new options to protect the brain against ethanol‐induced neurot...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.2882 |
| _version_ | 1798040606577000448 |
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| author | Yanxia Ding Yantong Wu Juan Chen Zhaoli Zhou Bing Zhao Rihong Zhao Yuzi Cui Qin Li Yue Cong |
| author_facet | Yanxia Ding Yantong Wu Juan Chen Zhaoli Zhou Bing Zhao Rihong Zhao Yuzi Cui Qin Li Yue Cong |
| author_sort | Yanxia Ding |
| collection | DOAJ |
| description | Abstract Ethanol is a principal ingredient of alcoholic beverages with potential neurotoxicity and genotoxicity, and the ethanol‐associated oxidative DNA damage in the central nervous system is well documented. Natural product may offer new options to protect the brain against ethanol‐induced neurotoxicity. The male flower of Eucommia ulmoides (EUF) Oliver has been extensively utilized as the tea, the healthy hot drink on the market. In this study, 19 constituents in the effective fraction of EUF were identified by ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). In the single‐cell gel electrophoresis assay, EUF was observed to ameliorate DNA damage in mouse cerebellum and cerebral cortex caused by acute ethanol administration, which was further confirmed by the morphological observation. The protective effects of EUF were associated with increasing total superoxide dismutase (T‐SOD) and glutathione peroxidase (GSH‐PX) activities, and a decrease in nitric oxide (NO), malondialdehyde (MDA), 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), and kelch‐like ECH‐associated protein‐1 (Keap1) levels. Molecular docking results demonstrated that compounds 4, 7, 9, and 16 from EUF have a strong affinity to the Keap1 Kelch domain to hinder the interaction of nuclear factor‐erythroid 2‐related factor 2 (Nrf2) with Keap1. These findings suggest that EUF is a potent inhibitor of ethanol‐induced brain injury possibly via the inhibition of oxidative stress. |
| first_indexed | 2024-04-11T22:09:59Z |
| format | Article |
| id | doaj.art-5c74258717d94de386c49e25140fd457 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-11T22:09:59Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-5c74258717d94de386c49e25140fd4572022-12-22T04:00:35ZengWileyFood Science & Nutrition2048-71772022-08-011082794280310.1002/fsn3.2882Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortexYanxia Ding0Yantong Wu1Juan Chen2Zhaoli Zhou3Bing Zhao4Rihong Zhao5Yuzi Cui6Qin Li7Yue Cong8Institute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaState Key Laboratory of Crop Stress Adaptation and Improvement Henan Joint International Laboratory for Crop Multi‐Omics Research School of Life Sciences Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaInstitute of Pharmacy Engineering Center of Henan Province Eucommia ulmoides Cultivation and Utilization School of Pharmacy Henan University Kaifeng ChinaAbstract Ethanol is a principal ingredient of alcoholic beverages with potential neurotoxicity and genotoxicity, and the ethanol‐associated oxidative DNA damage in the central nervous system is well documented. Natural product may offer new options to protect the brain against ethanol‐induced neurotoxicity. The male flower of Eucommia ulmoides (EUF) Oliver has been extensively utilized as the tea, the healthy hot drink on the market. In this study, 19 constituents in the effective fraction of EUF were identified by ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). In the single‐cell gel electrophoresis assay, EUF was observed to ameliorate DNA damage in mouse cerebellum and cerebral cortex caused by acute ethanol administration, which was further confirmed by the morphological observation. The protective effects of EUF were associated with increasing total superoxide dismutase (T‐SOD) and glutathione peroxidase (GSH‐PX) activities, and a decrease in nitric oxide (NO), malondialdehyde (MDA), 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), and kelch‐like ECH‐associated protein‐1 (Keap1) levels. Molecular docking results demonstrated that compounds 4, 7, 9, and 16 from EUF have a strong affinity to the Keap1 Kelch domain to hinder the interaction of nuclear factor‐erythroid 2‐related factor 2 (Nrf2) with Keap1. These findings suggest that EUF is a potent inhibitor of ethanol‐induced brain injury possibly via the inhibition of oxidative stress.https://doi.org/10.1002/fsn3.2882cerebellumcerebral cortexDNA damageethanolEucommia ulmoides Oliver male floweroxidative stress |
| spellingShingle | Yanxia Ding Yantong Wu Juan Chen Zhaoli Zhou Bing Zhao Rihong Zhao Yuzi Cui Qin Li Yue Cong Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex Food Science & Nutrition cerebellum cerebral cortex DNA damage ethanol Eucommia ulmoides Oliver male flower oxidative stress |
| title | Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex |
| title_full | Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex |
| title_fullStr | Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex |
| title_full_unstemmed | Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex |
| title_short | Protective effect of Eucommia ulmoides Oliver male flowers on ethanol‐induced DNA damage in mouse cerebellum and cerebral cortex |
| title_sort | protective effect of eucommia ulmoides oliver male flowers on ethanol induced dna damage in mouse cerebellum and cerebral cortex |
| topic | cerebellum cerebral cortex DNA damage ethanol Eucommia ulmoides Oliver male flower oxidative stress |
| url | https://doi.org/10.1002/fsn3.2882 |
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