Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets
Abstract Obstructive sleep apnea syndrome (OSAS) and obesity go hand in hand in the majority of patients and both are associated with a systemic inflammation, immune disturbance and comorbidities such as cardiovascular disease. However, the unambiguous impact of OSAS and obesity on the individual in...
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| Format: | Article |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49921-5 |
| _version_ | 1797363366836043776 |
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| author | Ralph Pries Friederike Katharina Kosyna Reinhard Depping Kirstin Plötze-Martin Christian Lange Svenja Meyhöfer Sebastian M. Meyhöfer Jens U. Marquardt Karl-Ludwig Bruchhage Armin Steffen |
| author_facet | Ralph Pries Friederike Katharina Kosyna Reinhard Depping Kirstin Plötze-Martin Christian Lange Svenja Meyhöfer Sebastian M. Meyhöfer Jens U. Marquardt Karl-Ludwig Bruchhage Armin Steffen |
| author_sort | Ralph Pries |
| collection | DOAJ |
| description | Abstract Obstructive sleep apnea syndrome (OSAS) and obesity go hand in hand in the majority of patients and both are associated with a systemic inflammation, immune disturbance and comorbidities such as cardiovascular disease. However, the unambiguous impact of OSAS and obesity on the individual inflammatory microenvironment and the immunological consequences of human monocytes has not been distinguished yet. Therefore, aim of this study was to investigate the impact of OSAS and obesity related factors on the inflammatory microenvironment by performing flow cytometric whole blood measurements of CD14/CD16 monocyte subsets in normal weight OSAS patients, patients with obesity but without OSAS, and patients with OSAS and obesity, compared to healthy donors. Moreover, explicitly OSAS and obesity related plasma levels of inflammatory mediators adiponectin, leptin, lipocalin and metalloproteinase-9 were determined and the influence of different OSAS and obesity related factors on cytokine secretion and expression of different adhesion molecules by THP-1 monocytes was analysed. Our data revealed a significant redistribution of circulating classical and intermediate monocytes in all three patient cohorts, but differential effects in terms of monocytic adhesion molecules CD11a, CD11b, CD11c, CX3CR1, CD29, CD49d, and plasma cytokine levels. These data were reflected by differential effects of OSAS and obesity related factors leptin, TNFα and hypoxia on THP-1 cytokine secretion patterns and expression of adhesion molecules CD11b and CD49d. In summary, our data revealed differential effects of OSAS and obesity, which underlines the need for a customized therapeutic regimen with respect to the individual weighting of these overlapping diseases. |
| first_indexed | 2024-03-08T16:20:22Z |
| format | Article |
| id | doaj.art-5c75873caf3443b7b43d7ce4361ba42b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:20:22Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5c75873caf3443b7b43d7ce4361ba42b2024-01-07T12:22:54ZengNature PortfolioScientific Reports2045-23222024-01-0114111310.1038/s41598-023-49921-5Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsetsRalph Pries0Friederike Katharina Kosyna1Reinhard Depping2Kirstin Plötze-Martin3Christian Lange4Svenja Meyhöfer5Sebastian M. Meyhöfer6Jens U. Marquardt7Karl-Ludwig Bruchhage8Armin Steffen9Department of Otorhinolaryngology, University Hospital of Schleswig-HolsteinInstitute of Physiology, Working Group Hypoxia, Center for Structural and Cell Biology in Medicine, University of LübeckInstitute of Physiology, Working Group Hypoxia, Center for Structural and Cell Biology in Medicine, University of LübeckDepartment of Otorhinolaryngology, University Hospital of Schleswig-HolsteinDepartment of Otorhinolaryngology, University Hospital of Schleswig-HolsteinDepartment of Medicine 1, University Hospital of Schleswig-HolsteinInstitute for Endocrinology & Diabetes, University Hospital of Schleswig-HolsteinDepartment of Medicine 1, University Hospital of Schleswig-HolsteinDepartment of Otorhinolaryngology, University Hospital of Schleswig-HolsteinDepartment of Otorhinolaryngology, University Hospital of Schleswig-HolsteinAbstract Obstructive sleep apnea syndrome (OSAS) and obesity go hand in hand in the majority of patients and both are associated with a systemic inflammation, immune disturbance and comorbidities such as cardiovascular disease. However, the unambiguous impact of OSAS and obesity on the individual inflammatory microenvironment and the immunological consequences of human monocytes has not been distinguished yet. Therefore, aim of this study was to investigate the impact of OSAS and obesity related factors on the inflammatory microenvironment by performing flow cytometric whole blood measurements of CD14/CD16 monocyte subsets in normal weight OSAS patients, patients with obesity but without OSAS, and patients with OSAS and obesity, compared to healthy donors. Moreover, explicitly OSAS and obesity related plasma levels of inflammatory mediators adiponectin, leptin, lipocalin and metalloproteinase-9 were determined and the influence of different OSAS and obesity related factors on cytokine secretion and expression of different adhesion molecules by THP-1 monocytes was analysed. Our data revealed a significant redistribution of circulating classical and intermediate monocytes in all three patient cohorts, but differential effects in terms of monocytic adhesion molecules CD11a, CD11b, CD11c, CX3CR1, CD29, CD49d, and plasma cytokine levels. These data were reflected by differential effects of OSAS and obesity related factors leptin, TNFα and hypoxia on THP-1 cytokine secretion patterns and expression of adhesion molecules CD11b and CD49d. In summary, our data revealed differential effects of OSAS and obesity, which underlines the need for a customized therapeutic regimen with respect to the individual weighting of these overlapping diseases.https://doi.org/10.1038/s41598-023-49921-5 |
| spellingShingle | Ralph Pries Friederike Katharina Kosyna Reinhard Depping Kirstin Plötze-Martin Christian Lange Svenja Meyhöfer Sebastian M. Meyhöfer Jens U. Marquardt Karl-Ludwig Bruchhage Armin Steffen Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets Scientific Reports |
| title | Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets |
| title_full | Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets |
| title_fullStr | Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets |
| title_full_unstemmed | Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets |
| title_short | Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets |
| title_sort | distinguishing the impact of distinct obstructive sleep apnea syndrome osas and obesity related factors on human monocyte subsets |
| url | https://doi.org/10.1038/s41598-023-49921-5 |
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