Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals
<i>L. interrogans</i> belongs to highly invasive spirochaetes causing leptospirosis in mammals, including humans. During infection, this pathogen is exposed to various stressors, and therefore, it must reprogram its gene expression to survive in the host and establish infection in a shor...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2076-0817/12/4/512 |
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| author | Sabina Kędzierska-Mieszkowska Zbigniew Arent |
| author_facet | Sabina Kędzierska-Mieszkowska Zbigniew Arent |
| author_sort | Sabina Kędzierska-Mieszkowska |
| collection | DOAJ |
| description | <i>L. interrogans</i> belongs to highly invasive spirochaetes causing leptospirosis in mammals, including humans. During infection, this pathogen is exposed to various stressors, and therefore, it must reprogram its gene expression to survive in the host and establish infection in a short duration of time. Host adaptation is possible thanks to molecular responses where appropriate regulators and signal transduction systems participate. Among the bacterial regulators, there are σ factors, including ECF (extracytoplasmic function) σ factors. The <i>L. interrogans</i> genome encodes 11 putative ECF σ<sup>E</sup>-type factors. Currently, none of them has been characterized biochemically, and their functions are still unknown. One of them, LIC_10559, is the most likely to be active during infection because it is only found in the highly pathogenic <i>Leptospira</i>. The aim of this study was to achieve LIC_10559 overexpression to answer the question whether it may be a target of the humoral immune response during leptospiral infections. The immunoreactivity of the recombinant LIC_10559 was evaluated by SDS-PAGE, ECL Western blotting and ELISA assay using sera collected from <i>Leptospira</i>-infected animals and uninfected healthy controls. We found that LIC_10559 was recognized by IgG antibodies from the sera of infected animals and is, therefore, able to induce the host’s immune response to pathogenic <i>Leptospira</i>. This result suggests the involvement of LIC_10559 in the pathogenesis of leptospirosis. |
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| language | English |
| last_indexed | 2024-03-11T04:39:17Z |
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| series | Pathogens |
| spelling | doaj.art-5c763a7ad23842c69f0995323fdea9e22023-11-17T20:47:47ZengMDPI AGPathogens2076-08172023-03-0112451210.3390/pathogens12040512Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected AnimalsSabina Kędzierska-Mieszkowska0Zbigniew Arent1Department of General and Medical Biochemistry, Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, PolandUniversity Centre of Veterinary Medicine UAK, University of Agriculture in Krakow, 30-059 Krakow, Poland<i>L. interrogans</i> belongs to highly invasive spirochaetes causing leptospirosis in mammals, including humans. During infection, this pathogen is exposed to various stressors, and therefore, it must reprogram its gene expression to survive in the host and establish infection in a short duration of time. Host adaptation is possible thanks to molecular responses where appropriate regulators and signal transduction systems participate. Among the bacterial regulators, there are σ factors, including ECF (extracytoplasmic function) σ factors. The <i>L. interrogans</i> genome encodes 11 putative ECF σ<sup>E</sup>-type factors. Currently, none of them has been characterized biochemically, and their functions are still unknown. One of them, LIC_10559, is the most likely to be active during infection because it is only found in the highly pathogenic <i>Leptospira</i>. The aim of this study was to achieve LIC_10559 overexpression to answer the question whether it may be a target of the humoral immune response during leptospiral infections. The immunoreactivity of the recombinant LIC_10559 was evaluated by SDS-PAGE, ECL Western blotting and ELISA assay using sera collected from <i>Leptospira</i>-infected animals and uninfected healthy controls. We found that LIC_10559 was recognized by IgG antibodies from the sera of infected animals and is, therefore, able to induce the host’s immune response to pathogenic <i>Leptospira</i>. This result suggests the involvement of LIC_10559 in the pathogenesis of leptospirosis.https://www.mdpi.com/2076-0817/12/4/512immunoreactivityinfection<i>Leptospira</i>leptospirosispathogensigma factor |
| spellingShingle | Sabina Kędzierska-Mieszkowska Zbigniew Arent Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals Pathogens immunoreactivity infection <i>Leptospira</i> leptospirosis pathogen sigma factor |
| title | Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals |
| title_full | Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals |
| title_fullStr | Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals |
| title_full_unstemmed | Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals |
| title_short | Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from <i>Leptospira interrogans</i> with Sera from <i>Leptospira</i>-Infected Animals |
| title_sort | immunoreactivity of a putative ecf σ factor lic 10559 from i leptospira interrogans i with sera from i leptospira i infected animals |
| topic | immunoreactivity infection <i>Leptospira</i> leptospirosis pathogen sigma factor |
| url | https://www.mdpi.com/2076-0817/12/4/512 |
| work_keys_str_mv | AT sabinakedzierskamieszkowska immunoreactivityofaputativeecfsfactorlic10559fromileptospirainterrogansiwithserafromileptospiraiinfectedanimals AT zbigniewarent immunoreactivityofaputativeecfsfactorlic10559fromileptospirainterrogansiwithserafromileptospiraiinfectedanimals |