CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway
Summary: CD9 is a member of the tetraspanin protein family, which has been widely studied in inflammation and cancer, but not in pathological cardiac hypertrophy. In this study, we found that the expression of CD9 was increased in transaortic constriction (TAC) myocardial tissue. Knockdown of CD9 al...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223021478 |
| _version_ | 1827794239623266304 |
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| author | Yue Li Siyuan Fan Lingyao Kong Zhenxuan Hao Yanjun Zhou Jiahong Shangguan Lu Gao Mingdan Wang Yue Kang Xiangrao Li Kun Huang Chao Zhang Zhibo Liu |
| author_facet | Yue Li Siyuan Fan Lingyao Kong Zhenxuan Hao Yanjun Zhou Jiahong Shangguan Lu Gao Mingdan Wang Yue Kang Xiangrao Li Kun Huang Chao Zhang Zhibo Liu |
| author_sort | Yue Li |
| collection | DOAJ |
| description | Summary: CD9 is a member of the tetraspanin protein family, which has been widely studied in inflammation and cancer, but not in pathological cardiac hypertrophy. In this study, we found that the expression of CD9 was increased in transaortic constriction (TAC) myocardial tissue. Knockdown of CD9 alleviated damage to cardiac function in the TAC model and reduced heart weight, cardiomyocyte size, and degree of fibrosis, and vice versa. Mechanistically, co-immunoprecipitation results showed that CD9 and GP130 can bind to each other in cardiomyocytes, and knockdown of CD9 can reduce the protein level of GP130 and the phosphorylation of STAT3 in vivo and in vitro, and vice versa. GP130 knockdown reversed the aggravating effects of CD9 on pathological cardiac hypertrophy. Therefore, we conclude that CD9 exacerbates pathological cardiac hypertrophy by regulating the GP130/STAT3 signaling pathway and may serve as a therapeutic target for pathological cardiac hypertrophy. |
| first_indexed | 2024-03-11T18:29:55Z |
| format | Article |
| id | doaj.art-5c77285570994ad0a1742ebab8f00fb1 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T18:29:55Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-5c77285570994ad0a1742ebab8f00fb12023-10-13T11:05:38ZengElsevieriScience2589-00422023-11-012611108070CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathwayYue Li0Siyuan Fan1Lingyao Kong2Zhenxuan Hao3Yanjun Zhou4Jiahong Shangguan5Lu Gao6Mingdan Wang7Yue Kang8Xiangrao Li9Kun Huang10Chao Zhang11Zhibo Liu12Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Gene Hospital of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaCardiovascular Center, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaPulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, ChinaDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China; Corresponding authorInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Corresponding authorGene Hospital of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Corresponding authorSummary: CD9 is a member of the tetraspanin protein family, which has been widely studied in inflammation and cancer, but not in pathological cardiac hypertrophy. In this study, we found that the expression of CD9 was increased in transaortic constriction (TAC) myocardial tissue. Knockdown of CD9 alleviated damage to cardiac function in the TAC model and reduced heart weight, cardiomyocyte size, and degree of fibrosis, and vice versa. Mechanistically, co-immunoprecipitation results showed that CD9 and GP130 can bind to each other in cardiomyocytes, and knockdown of CD9 can reduce the protein level of GP130 and the phosphorylation of STAT3 in vivo and in vitro, and vice versa. GP130 knockdown reversed the aggravating effects of CD9 on pathological cardiac hypertrophy. Therefore, we conclude that CD9 exacerbates pathological cardiac hypertrophy by regulating the GP130/STAT3 signaling pathway and may serve as a therapeutic target for pathological cardiac hypertrophy.http://www.sciencedirect.com/science/article/pii/S2589004223021478Cardiovascular medicinePathophysiologyMolecular physiology |
| spellingShingle | Yue Li Siyuan Fan Lingyao Kong Zhenxuan Hao Yanjun Zhou Jiahong Shangguan Lu Gao Mingdan Wang Yue Kang Xiangrao Li Kun Huang Chao Zhang Zhibo Liu CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway iScience Cardiovascular medicine Pathophysiology Molecular physiology |
| title | CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway |
| title_full | CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway |
| title_fullStr | CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway |
| title_full_unstemmed | CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway |
| title_short | CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway |
| title_sort | cd9 exacerbates pathological cardiac hypertrophy through regulating gp130 stat3 signaling pathway |
| topic | Cardiovascular medicine Pathophysiology Molecular physiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223021478 |
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