Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence
Tea is one of the most well-known, healthy beverages in the world. Tea plants produce caffeine as a secondary metabolite. Colletotrichum camelliae is one of the most important microbes frequently isolated from tea fields, and it causes anthracnose disease in tea plant. In the present work, we perfor...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1038806/full |
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| author | Shengnan He Xiaoyan Qiao Shuhan Zhang Jinglin Xia Lei Wang Shouan Liu Shouan Liu |
| author_facet | Shengnan He Xiaoyan Qiao Shuhan Zhang Jinglin Xia Lei Wang Shouan Liu Shouan Liu |
| author_sort | Shengnan He |
| collection | DOAJ |
| description | Tea is one of the most well-known, healthy beverages in the world. Tea plants produce caffeine as a secondary metabolite. Colletotrichum camelliae is one of the most important microbes frequently isolated from tea fields, and it causes anthracnose disease in tea plant. In the present work, we performed molecular microbiology and transcriptomic analyses of the C. camelliae - tea plant interaction to investigate the mechanism of fungal virulence and plant defense. Upon infection of tea plant with C. camelliae, we observed alterations in the expression of fungal transcripts, including those of many genes associated with caffeine metabolism, such as those encoding various transporters, xanthine dehydrogenase, and urate oxidase (UOX). In particular, the deletion of C. camelliae urate oxidase (CcUOX), which is involved in the caffeine metabolism pathway, reduced fungal tolerance to caffeine, and impaired fungal virulence. CcUOX is involved in caffeine metabolism by the degradation of uric acid contents. C. camelliaeΔCcUOX mutants impaired uric acid degradation in vivo. The CcUOX gene was cloned from C. camelliae, overexpressed in Escherichia coli, and the recombinant CcUOX protein displayed maximum activity at 30°C and a pH of 4.0. The recombinant CcUOX efficiently reduced uric acid in vitro suggesting a promising application in caffeine-contaminated environment management and in producing food with low purine contents to prevent uric acid related human diseases, such as hyperuricemia and gout. |
| first_indexed | 2024-04-11T00:58:52Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T00:58:52Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-5c7999659f19486dbe724f27d607be242023-01-04T19:44:14ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-01-01910.3389/fnut.2022.10388061038806Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulenceShengnan He0Xiaoyan Qiao1Shuhan Zhang2Jinglin Xia3Lei Wang4Shouan Liu5Shouan Liu6Laboratory of Tea and Medicinal Plant Biology, College of Plant Sciences, Jilin University, Changchun, ChinaGuangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization/Guangdong Academy of Agricultural Sciences Tea Research Institute, Guangzhou, ChinaLaboratory of Tea and Medicinal Plant Biology, College of Plant Sciences, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Biology, College of Plant Sciences, Jilin University, Changchun, ChinaInstitute of Chemical and Industrial Bioengineering, Jilin Engineering Normal University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Biology, College of Plant Sciences, Jilin University, Changchun, ChinaLaboratory of Molecular Plant Pathology, College of Plant Sciences, Jilin University, Changchun, ChinaTea is one of the most well-known, healthy beverages in the world. Tea plants produce caffeine as a secondary metabolite. Colletotrichum camelliae is one of the most important microbes frequently isolated from tea fields, and it causes anthracnose disease in tea plant. In the present work, we performed molecular microbiology and transcriptomic analyses of the C. camelliae - tea plant interaction to investigate the mechanism of fungal virulence and plant defense. Upon infection of tea plant with C. camelliae, we observed alterations in the expression of fungal transcripts, including those of many genes associated with caffeine metabolism, such as those encoding various transporters, xanthine dehydrogenase, and urate oxidase (UOX). In particular, the deletion of C. camelliae urate oxidase (CcUOX), which is involved in the caffeine metabolism pathway, reduced fungal tolerance to caffeine, and impaired fungal virulence. CcUOX is involved in caffeine metabolism by the degradation of uric acid contents. C. camelliaeΔCcUOX mutants impaired uric acid degradation in vivo. The CcUOX gene was cloned from C. camelliae, overexpressed in Escherichia coli, and the recombinant CcUOX protein displayed maximum activity at 30°C and a pH of 4.0. The recombinant CcUOX efficiently reduced uric acid in vitro suggesting a promising application in caffeine-contaminated environment management and in producing food with low purine contents to prevent uric acid related human diseases, such as hyperuricemia and gout.https://www.frontiersin.org/articles/10.3389/fnut.2022.1038806/fullColletotrichum camelliaetea plantcaffeine metabolismurate oxidasepurine alkaloids |
| spellingShingle | Shengnan He Xiaoyan Qiao Shuhan Zhang Jinglin Xia Lei Wang Shouan Liu Shouan Liu Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence Frontiers in Nutrition Colletotrichum camelliae tea plant caffeine metabolism urate oxidase purine alkaloids |
| title | Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| title_full | Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| title_fullStr | Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| title_full_unstemmed | Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| title_short | Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| title_sort | urate oxidase from tea microbe colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence |
| topic | Colletotrichum camelliae tea plant caffeine metabolism urate oxidase purine alkaloids |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1038806/full |
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